Abstract:A simplified method for preparing blood serum and plasma for Zeeman electrothermal atomic absorption spectrometry is described and applied to the measurement of chromium in human serum and plasma. This enzymatic degradation with bacterial protease (EC 3.4.21.3) requires little laboratory apparatus, decreases the work and time of sample preparation, and obviates some potential sources of contamination. We used bovine reference serum (USDA No. 7292) to validate Cr concentration. There was less Cr in serum than i… Show more
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