Rapid Enrichment and Detection of Silk Residues from Tombs by Double-Antibody Sandwich ELISA Based on Immunomagnetic Beads

Abstract

The extraction and identification of silk residues in tombs is of great significance for studying the distribution and spread of early silk. However, the complex organic matter in the tomb hinders the accurate identification of silk. In this study, a doubleantibody sandwich enzyme-linked immunosorbent assay (ELISA) based on immunomagnetic beads (IMBs) was developed for the rapid enrichment and detection of silk residues. The doubleantibody sandwich ELISA method established by pairing the IMBs prepared by the silk fibroin monoclonal antibody SF-3 and the silk fibroin monoclonal-labeled antibody bio-SF-1 had the highest detection sensitivity, with a linear detection range of 10 to 10 4 ng mL −1 and a detection limit of 5.12 ng mL −1 . This method was excellent in the extraction and analysis of silk residues from archaeological imprints and soil samples and successfully identified silk residues in samples at the final stage of silk degradation (physical invisible silk). The proteomics analysis results demonstrated the feasibility and practicability of this method.

Comparison and Research Progress of Protein Detection Technology for Cultural Relic Materials

“…Through animal immunology, specific antibodies to various silks were developed, and distinct species' silk proteins were successfully identified. In 2021, Zheng et al [86] created a technique for quick enrichment and detection of silk residues based on immune-labeled magnetic beads and ELISA. The detection threshold was at 5.12 ng/mL.…”