Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea Wall

Influence of Culture Media and Explants on in vitro Regeneration Efficiency of Elite Guava Cultivars

“…MS medium consisted of 1.5 mg/l TDZ had lowest regeneration efficiency for Lucknow-49 (4.85%) and MS medium with 1.5 mg/l TDZ and 0.5 mg/l IAA showed lower regeneration efficiency of 5.37% in Allahabad Safeda (Table 1). Direct shoot proliferation was found to be dependent on synergistic effect of cytokinins along with auxin in the medium which evoked good results for shoot proliferation (Kumar et al, 2013). The maximum shoot proliferation and number of shoots was formed directly from node explants due to the presence of preexisting axillary primordia which proliferated into shoots under adequate amount of cytokinin along with small amount of auxins supplied in nutrient medium which nullified the effect of apical dominance and thus enhanced the proliferation of axillary buds (Kumar et al, 2017).…”

Changes in biochemical constituents, enzyme activities and protein profiles during root-shoot differentiation in callus culture of Dioscorea alata

“…However, NAA (2.5 µM) along with 2, 4-D (2.0 µM), induced good growth of callus as well as root and shoot formation within 28 and 31 days after inoculation, respectively. Regeneration of shoot was obtained on MS medium supplemented with BAP (2.0 µM) and NAA (0.5 µM), while green patches were formed before the appearance of shoot formation (Kumar et al 2013). The visual appearance of shoots was observed after 12 th day of subculture (Table 3).…”

“…Underground tubers (yams) are the source of phytochemicals used to treat health ailments such as inflammation, joint pain, diabetes, infection and dysmenorrhea. Analysis of various cellular metabolites during organ differentiation in callus provides a reasonable and promising approach towards an understanding of the biochemical basis of organogenesis (organ development/ plant regeneration) (Kumar et al 2013). In spite of considerable achievements in genetic transformation, least is known about plant cell differentiation in cultures as well as the underlying molecular basis of in vitro differentiation (Goyal et al 2009).…”