Rapid DNA typing of class II HLA antigens using the polymerase chain reaction and reverse dot blot hybridization

Buyse, Inge M.; Decorte, Ronny; Baens, Mathijs; Cuppens, Harry; Sémana, G.; Emonds, Marie‐Paule; Marynen, Peter; Cassiman, Jean‐Jacques

doi:10.1111/j.1399-0039.1993.tb01970.x

Cited by 181 publications

(88 citation statements)

References 39 publications

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“…Further studies of patients with QFS, Q fever endocarditis and acute Q fever without chronic complications are required to fully establish the concept. 16 Values other than HLA-DR-11 did not reach significance. Variation in immune response genes and chronic Q fever KJ Helbig et al…”

Section: Discussionmentioning

confidence: 77%

Variation in immune response genes and chronic Q fever. Concepts: preliminary test with post-Q fever fatigue syndrome

Helbig¹,

Heatley

Harris³

et al. 2003

Genes Immun

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Acute primary Q fever is followed by various chronic sequelae. These include subacute Q fever endocarditis, granulomatous reactions in various organs or a prolonged debilitating post-infection fatigue syndrome (QFS). The causative organism, Coxiella burnetii, persists after an initial infection. The differing chronic outcomes may reflect variations within cytokine and accessory immune control genes which affect regulation of the level of persistence. As a preliminary test of the concept we have genotyped QFS patients and controls for gene variants spanning 15 genes and also examined HLA-B and DR frequencies. QFS patients exhibited a significantly increased frequency of HLA-DR-11 compared with controls and also significant differences in allelic variant frequencies within the NRAMP, and IFNg genes. These results indicate a possible genetic role in the expression of overt chronic Q fever. Further studies will be undertaken to increase sample sizes, to survey other forms of chronic Q fever and to examine Q fever patients who have recovered without sequelae.

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Section: Discussionmentioning

confidence: 77%

Variation in immune response genes and chronic Q fever. Concepts: preliminary test with post-Q fever fatigue syndrome

Helbig¹,

Heatley

Harris³

et al. 2003

Genes Immun

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“…The strips were analyzed and the DRB1, 3, 4, 5, DQB1, and DPB1 molecules assigned according to the pattern of PCR products hybridization. 10 The HLA restriction of peptide recognition by T cells was determined using allogeneic APC (HLA-matched or -mismatched with the T-cell donor) to present the peptide to T-cell lines or clones.…”

Section: Production Of Core-specific T-cell Clones and Olygoclonal T-mentioning

confidence: 99%

Conserved hepatitis C virus sequences are highly immunogenic for CD4+ T cells: Implications for vaccine development

et al. 1999

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The HLA class II-restricted T-cell response to hepatitis C virus (HCV) antigens is believed to influence the final outcome of hepatitis C, because it is vigorous in patients who recover from acute hepatitis C, but it is weak in those who develop a chronic infection. For this reason, exogenous stimulation of T-cell responses in chronic HCV infection may represent a strategy to cure patients with chronic hepatitis C by approximating the vigor of their T-cell reactivity to that of patients who succeed in recovering from hepatitis. It may also be a preventive approach to avoid spread of the virus by facilitating the development of a vigorous protective response at the very early stages of infection. T-cell-based vaccines composed of immunodominant, promiscuous, and conserved T-cell epitopes may represent a powerful tool to achieve optimal stimulation of the T-cell reactivity. To identify HLA class II-restricted T-cell epitopes useful for this purpose, 22 subjects with acute HCV infection were studied and followed for an average time of 29 months. Eight of them recovered from hepatitis, and 14 developed a chronic infection. Overlapping 20-mer peptides covering the entire core and NS4 antigens and a panel of peptides representing highly conserved regions of core, NS3, NS4, and NS5 were used. By direct peripheral blood T-cell stimulation and by finespecificity analysis of HCV-specific T-cell lines and clones, highly immunogenic T-cell epitopes were identified within core, NS3, and NS4. All these epitopes are immunodominant and highly conserved among the known HCV isolates. Moreover, they are promiscuous, because they can be presented to T cells by different HLA class II molecules. Immunodominance, sequence conservation, and promiscuity make these epitopes ideal components of preventive or therapeutic T-cell-based vaccines against HCV. (HEPATOL-OGY 1999;30:1088-1098.)The hepatitis C virus (HCV) is a RNA virus that can cause either acute self-limited infections or, more frequently, chronic liver cell injury leading to cirrhosis and hepatocellular carcinoma in a high proportion of cases. 1 The mechanisms responsible for persistence or viral clearance are still largely unknown. However, recent studies reported different patterns of proliferative T-cell response and cytokine production by antigen-specific CD4 ϩ T cells in patients with self-limited or chronically evolving HCV infection. [2][3][4] Patients with selflimited acute hepatitis show more vigorous peripheral blood proliferative T-cell responses to HCV proteins, predominantly sustained by Th1-like lymphocytes able to secrete proinflammatory cytokines (interferon gamma [IFN-␥] and interleukin-2 [IL-2]). Patients with chronic evolution of infection display weaker and less efficient proliferative T-cell responses suggesting that the intensity of the T-cell reactivity at the early stages of infection may be critical to limit the spread of the virus within the infected host and to keep viral replication under control. 2,3 In continuation of our previous studies suggestin...

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“…22 HLA-A, -B, -C, -DRB, -DQA1 and -DQB1 low-or high-resolution genotyping was performed by polymerase chain reaction, with sequence-specific primers (SSP-PCR), 23 using Dynal (Oslo, Norway) commercial reagents, according to the manufacturer's instructions. HLA-DPB1 high-resolution genotyping was performed by PCR and reverse-hybridization with sequence-specific oligonucleotide probes (SSO-PCR), 24 using the INNO-LiPA DPB kit (Innogenetics, Ghent, Belgium), according to the manufacturer's instructions. All PCR reactions were carried out in a GeneAmp PCR System 9600 (Perkin Elmer, Norwalk, CT, USA).…”

Section: Hla Typing Of Patients and Donorsmentioning

confidence: 99%

Unrelated donor cord blood transplantation in adults with chronic myelogenous leukemia: results in nine patients from a single institution

Sanz

Saavedra

Jiménez

et al. 2001

Bone Marrow Transplant

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Summary:The potential role of unrelated donor cord blood transplantation (UD-CBT) in adults is not well established. We report the results of UD-CBT in nine adult patients with chronic myeloid leukemia (CML). The median age was 27 years (range, 19-41 years), and the median weight was 62 kg (range, 45-78 kg). At transplant, six patients were in chronic phase (five in first, and one in second), two in blast crisis, and one in accelerated phase. Eight had received intensive chemotherapy, and three had undergone autologous peripheral blood hematopoietic stem cell transplantation. Four had received interferon with no cytogenetic response, and only three underwent UD-CBT within 1 year of diagnosis. After serological typing for class I antigens, and high-resolution DNA typing for DRB1, the degree of HLA match between patients and cord blood (CB) units was 4/6 in six cases and 5/6 in three cases. The median number of nucleated cells infused was 1.7 × 10 7 /kg (range, 1.2 to 4.9 × 10 7 /kg), and was above 2 × 10 7 /kg in only two cases. All patients received thiotepa, busulfan, cyclophosphamide and anti-thymocyte globulin as conditioning; cyclosporine and prednisone for graft-versushost disease (GVHD) prophylaxis; and G-CSF from day +7 until engraftment. All seven evaluable cases engrafted. The median time to reach an absolute neutrophil count у0.5 × 10 9 /l and у1 × 10 9 /l was 22 days (range, 19-52 days) and 28 days (range, 23-64 days), respectively. In the four patients evaluable for platelet recovery time to levels of у20 × 10 9 platelets/l, у50 × 10 9 platelets/l, and у100 × 10 9 platelets/l, these ranged from 50 to 128 days, 60 to 139 days, and 105 to 167 days, respectively. Three patients developed acute GVHD above grade II, and three of the five patients at risk developed extensive chronic GVHD. Four patients, all transplanted in chronic phase, remain alive in mol-

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Rapid DNA typing of class II HLA antigens using the polymerase chain reaction and reverse dot blot hybridization

Cited by 181 publications

References 39 publications

Variation in immune response genes and chronic Q fever. Concepts: preliminary test with post-Q fever fatigue syndrome

Variation in immune response genes and chronic Q fever. Concepts: preliminary test with post-Q fever fatigue syndrome

Conserved hepatitis C virus sequences are highly immunogenic for CD4+ T cells: Implications for vaccine development

Unrelated donor cord blood transplantation in adults with chronic myelogenous leukemia: results in nine patients from a single institution

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