Rapid discrimination of F1 hybrid seeds from their parental lines and selection of protein-rich corn lines for silage corn breeding using FT-IR spectroscopy combined by multivariate analysis

Nahm, Seok Hyeon; Yang, Seung Gyun; Kim, Suk Weon; Min, Byung Whan

doi:10.1007/s12892-015-0108-7

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Among these methods, SRAP (sequence-related amplified polymorphism) is recognized to be a useful and inexpensive molecular marker technique for hybrid identification and genetic relationship studies [18][19][20] as compared to other approaches based on next generation sequencing (NGS) techniques [5]. Furthermore, Fourier-transform infrared (FT-IR) spectroscopy is also an easy, low-cost, and rapid method for analyzing the molecular content of complex samples based on the vibrations of both functional groups and highly polar bonds of sample components even on a large scale [21,22]. It can easily be applied also for hundreds of progenies, since the only chemical substance used is KBr, which can be purchased at accessible price.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy

Hârța

Borsai

Muntean

et al. 2020

Plants

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The genetic relationship among three Streptocarpus parents and twelve F1 hybrids was assessed using sequence-related amplified polymorphism (SRAP) molecular markers and Fourier-transform infrared (FT-IR) spectroscopy. Both methods were able to discriminate F1 hybrids and parents as revealed by cluster analysis. For hybrid identification, the type III SRAP marker was the most effective due to the presence of male-specific bands in the hybrids. Different behaviors in the biochemical variability of DNA samples have been observed by FT-IR spectral analysis, which might be attributed to the inherent nature of the genomic DNA from parents and their F1 progenies. Mantel test was also carried out to compare morphological, SRAP, and FT-IR results based on genetic distances. The highest correlation coefficient was found between morphological and SRAP marker distances (R = 0.607; p ≤ 0.022). A lower correlation was observed between the morphological and FT-IR distance matrix (R = 0.231; p ≤0.008). Moreover, a positive correlation was found between the distances generated with SRAP and FT-IR analyses (R = 0.026) but was not statistically significant. These findings show that both SRAP and FT-IR techniques combined with morphological descriptions can be used effectively for nonconventional breeding programs for Streptocarpus to obtain new and valuable varieties.

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Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy

Hârța

Borsai

Muntean

et al. 2020

Plants

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Rapid Screening of Maize Inbred Lines Based on NIR-MIR Spectral Characteristics and Small-molecule Metabolites

Yang

Liu

et al. 2018

J. Crop Sci. Biotechnol.

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Identification of True and False Aksu Apple Based on NIRS and PLS-DA

Yang

Liu

et al. 2019

IOP Conf. Ser.: Earth Environ. Sci.

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A method for the identification of true and false Aksu Red Fuji apple varieties based on near-infrared spectroscopy (NIRS) and partial least squares discrimination (PLS-DA) was proposed and established. Authentic Xinjiang Aksu Red Fuji and ordinary Red Fuji (Shandong and Shanxi Red Fuji) were purchased from supermarkets. There were 42 samples in each variety, with totaling 126 samples. Near infrared diffuse reflectance spectra of all samples were collected in the range of 4000-12000 cm−1. On the basis of studying the characteristics of NIR spectra, the principal component analysis (PCA) was carried out. It is pointed out that PCA method cannot fully realize the identifcation of three kinds Red Fuji Apples. Finally, the partial least squares discriminant analysis model of three kinds Red Fuji Apples was established, and the correct discrimination rates of the three kinds Red Fuji Apples were 100% for samples in calibration set and prediction set.

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Rapid discrimination of F1 hybrid seeds from their parental lines and selection of protein-rich corn lines for silage corn breeding using FT-IR spectroscopy combined by multivariate analysis

Cited by 3 publications

References 25 publications

Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy

Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy

Rapid Screening of Maize Inbred Lines Based on NIR-MIR Spectral Characteristics and Small-molecule Metabolites

Identification of True and False Aksu Apple Based on NIRS and PLS-DA

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