Rapid discovery of absorbed constituents and metabolites in rat plasma after the oral administration of <i>Zi Shen Wan</i> using high‐throughput UHPLC–MS with a multivariate analysis approach

Li, Xianna; Zhang, Aihua; Sun, Hui; Song, Yanhua; Zou, Di; Wang, Xijun

doi:10.1002/jssc.201600812

Cited by 35 publications

(19 citation statements)

References 27 publications

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“…Due to lack of in‐depth component analysis, its clinical application is limited to a large extent. Li et al () adopted the UPLC‐MS platform to demonstrate the chemical composition of Zishen Wan; ninety‐two compounds were characterized, among them, 59 constituents came from Phellodendri Amurensis Cortex, 25 from Anemarrhenae Rhizoma, and 8 from Cinnamomi Cortex.…”

Section: Ms In Herbal Ingredinetsmentioning

confidence: 99%

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Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Wang

Ren

Zhang

et al. 2019

Mass Spectrometry Reviews

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102

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Current evidence shows that herbal medicines could be beneficial for the treatment of various diseases. However, the complexities present in chemical compositions of herbal medicines are currently an obstacle for the progression of herbal medicines, which involve unclear bioactive compounds, mechanisms of action, undetermined targets for therapy, non‐specific features for drug metabolism, etc. To overcome those issues, metabolomics can be a great to improve and understand herbal medicines from the small‐molecule metabolism level. Metabolomics could solve scientific difficulties with herbal medicines from a metabolic perspective, and promote drug discovery and development. In recent years, mass spectrometry‐based metabolomics was widely applied for the analysis of herbal constituents in vivo and in vitro. In this review, we highlight the value of mass spectrometry‐based metabolomics and metabolism to address the complexity of herbal medicines in systems pharmacology, and to enhance their biomedical value in biomedicine, to shed light on the aid that mass spectrometry‐based metabolomics can offer to the investigation of its active ingredients, especially, to link phytochemical analysis with the assessment of pharmacological effect and therapeutic potential. © 2019 Wiley Periodicals, Inc. Mass Spec Rev

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Section: Ms In Herbal Ingredinetsmentioning

confidence: 99%

“…Due to lack of in-depth component analysis, its clinical application is limited to a large extent. Li et al (2016b) , and Salviae Miltiorrhizae Radix Rhizoma, commonly used in the recovery and delay of chronic kidney disease. However, the chemical composition of Bupi Yishen Formula has not been fully elucidated.…”

Section: A Qualitative Analysismentioning

confidence: 99%

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Wang

Ren

Zhang

et al. 2019

Mass Spectrometry Reviews

Self Cite

102

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“…However, most studies of network pharmacology have gathered compounds from databases to explore action mechanisms. Previous studies have proved that the main active constituents are probably the components and metabolites absorbed or metabolized into the blood after administration (Li, Zhang, Sun, et al, ). Therefore, the absorbed components in vivo were selected to perform network pharmacology analysis to overcome this problem.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive investigation of mechanism and effective ingredients of Fangji Huangqi Tang by serum pharmacochemistry and network pharmacology

Yang

Chen

et al. 2020

Biomedical Chromatography

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Fangji Huangqi Tang (FHT), has been reported to show effects on nephrotic syndrome, but its mechanism of action and bioactive components have not yet been determined. In this study, a method using UPLC–HRMS/MS was established for the detection and identification of the chemical constituents and metabolites absorbed into the blood. Absorbed components in serum were then used for the network pharmacology analysis to deduce the mechanism and effective components. A total of 86 compounds were identified or tentatively characterized. Based on the same instrumental conditions, 85 compounds were found in rat serum after oral administration of FHT, including 22 prototypes and 63 metabolites. Network pharmacology analysis showed that absorbed components, such as (3R)‐2′,3′,4′,7‐tetrahydroxyisoflavan, astrapterocarpan, cycloastragenol, 7,2′‐dihydroxy‐3′,4′‐dimethoxyisoflavan, astragaloside IV, astrapterocarpan glucoside and glycyrrhetinic acid, could be responsible for the pharmacological activity of nephrotic syndrome by regulating the VEGF signaling pathway, focal adhesion and MAPK signaling pathway. Furthermore, the pathway‐target network showed that the MAPK1, AKT2 and CDC42 were involved in the signal pathways above. This study provides a scientific basis for the mechanism and effective ingredients of FHT.

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“…Chemical analysis is the basis to appreciate those multiple phytochemicals and metabolites of TCM formulas. Thus, a rapid and reliable method for comprehensive identification of the chemical constituents and metabolites is needed for the modernization of TCM formula .…”

Section: Introductionmentioning

confidence: 99%

Rapid characterization of chemical constituents and metabolites of Qi‐Jing‐Sheng‐Bai granule by using UHPLC–Q‐TOF‐MS

Lin

Tian

et al. 2018

J of Separation Science

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Qi-Jing-Sheng-Bai granule is an effective traditional Chinese medicine formula that has been widely used for the treatment of leukopenia post radiotherapy or chemotherapy. However, its chemical constituents were still unclear, which hindered interpreting bioactive constituents and studying integrative mechanisms. In this study, we developed a three-step strategy to characterize the chemical constituents and metabolites of Qi-Jing-Sheng-Bai by using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. As a result, a total of 143 compounds, including 56 flavonoids, 51 saponins, and 36 other compounds, of which contained six pairs of isomers, were tentatively identified and characterized via reference standards and by comparing mass spectrometry data with literature. After oral administration of 15 g/kg Qi-Jing-Sheng-Bai, a number of 42 compounds including 24 prototype compounds and 18 metabolites have been detected in the serum of rats. This work serves as the first reference for Qi-Jing-Sheng-Bai chemical components and metabolites. Moreover, it provided a rapid and valid analytical strategy for characterization of the chemical compounds and metabolites of traditional Chinese medicine formula.

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Rapid discovery of absorbed constituents and metabolites in rat plasma after the oral administration of Zi Shen Wan using high‐throughput UHPLC–MS with a multivariate analysis approach

Cited by 35 publications

References 27 publications

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Comprehensive investigation of mechanism and effective ingredients of Fangji Huangqi Tang by serum pharmacochemistry and network pharmacology

Rapid characterization of chemical constituents and metabolites of Qi‐Jing‐Sheng‐Bai granule by using UHPLC–Q‐TOF‐MS

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