Rapid differentiation of glomerular and tubular proteinuria by sodium dodecyl sulfate polyacrylamide gel electrophoresis

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis is a method allowing the separation of proteins according to their molecular sizes. For this reason this method is rather useful for the classification of proteinurias as tubular, glomerular, or mixed. In the present study a good correlation was observed between the results of urine electrophoresis in sodium dodecyl sulphate-polyacrylamide gel and both clinical and histopathological data from 32 patients with different forms of renal disease.

Section: Introductionmentioning

confidence: 99%

Analytical Characterization of Urinary Proteins by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis in Renal Disease

Pires¹,

Cunha²,

Virella³

et al. 1975

“…For proteins such as albumin, the error due to other proteins might approximate 10-20%: but for proteins appearing in smaller amounts electrophoretic quantitation may give spurious results. Similar arguments hold for gel media such as polyacrylamide [51]; with this method the superior separation makes this type of error somewhat less likely, but quantitation is more difficult.…”

mentioning

confidence: 91%

“…The considerable overlap between the groups of protein fractions makes fine analysis difficult [52], although it has been attempted. The technique of polyacrylamide gel electrophoresis offers the advantage of resolution better than either gel fil tration or ultra-centrifugation, and more proteins can be resolved [51]. Accurate quantification has not yet been achieved to make it comparable to either of the above methods.…”

mentioning

confidence: 99%

Methods Used for the Analysis of Proteins in the Urine

Pesce¹

1974

Self Cite

Measurement of urinary proteins in proteinuric patients is a complex problem since the concentration, types of protein excreted, and presence of waste products vary considerably. Each method of quantitatíng urinary proteins makes certain assumptions and care must be taken to eliminate artifacts in the determinations. Concentration of urinary proteins without large losses of either all or certain ones must be achieved to quantify specific proteins. Currently, gel filtration and immunochemical methods are most often used for estimation of specific proteins or patterns of all proteins excreted. When combined, these two methods yield fairly rigorous results for the characterization and quantitation of known proteins. This approach has shown that the concept of selectivity of protein excretion does not hold over the protein size spectrum. However, the procedure is time-consuming and therefore is suited only for research studies. A more valid model to sort out the pathophysiologic basis of protein excretion may be a technique for examination of individual proteins or those of specific groups to distinguish between glomerular and tubular proteinuria. A suitable technique for this approach, because of its convenience, is that of protein electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate.

“…Following concentration, the urines were subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, according to a modification [15] of the method previously described [14], The major modification was that the gel was heated at 55 °C for several hours during the destaining procedure in order to decrease the background staining. Although this did not lead to complete elimination of background, this destaining procedure improved the quality of the gels substantially so that it was possible to scan the dried gels with a Helena Quick Scan electrophoresis densito meter to quantitate the relative amounts of albumin and low molecular weight (LMW) proteins present.…”

Section: Polyacrylamide Gel Electrophoresismentioning

confidence: 99%

“…With the use of polyacryl amide gel electrophoresis in the presence of the detergent sodium dodecyl sulfate [14], al bumin and proteins of a molecular size less than albumin can be measured semiquantita-lively [15]. We report the application of this method to the study of urine proteins following transplantation, with specific reference to that occurring in acute tubular necrosis (ATN) and acute rejection.…”

Section: Introductionmentioning

confidence: 99%

Proteinuria following Renal Transplantation

Sethi¹,

First²,

Pesce³

et al. 1977

Proteinuria was studied in ten renal allograft recipients; it was defined as: (a) glomerular – characterized by predominant albumin excretion; (b) tubular – significant excretion of both albumin and low molecular weight (LMW) proteins; and (c) glomerulo-tubular or mixed type, a combination of the two. LMW protein and albumin were quantitated by polyacrylamide gel electrophoresis with sodium dodecyl sulfate. In the immediate posttransplant period, LMW protein and albumin excretion, expressed as a percentage of creatinine clearance, were high, revealing a mixed pattern, and excretion of both protein classes was higher than during both acute tubular necrosis and acute rejection crisis. Tubular proteinuria was observed in acute tubular necrosis; a glomerulo-tubular or mixed pattern of protein excretion in acute rejection crises.