Rapid Differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades

Stegger, Marc; Liu, Cindy Meng Hsin; Larsen, Jesper; Soldanova, Katerina; Aziz, Maliha; Contente-Cuomo, Tania; Petersen, Andreas; Vandendriessche, Stien; Jiménez, J. Natalia; Mammina, Caterina; Belkum, Alex van; Salmenlinna, Saara; Laurent, François; Skov, Robert; Larsen, Anders A.R.; Andersen, Paal Skytt; Price, Lance L.B.

doi:10.1371/journal.pone.0079645

Cited by 84 publications

(94 citation statements)

References 29 publications

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“…Other STs described in pigs are ST541, ST1965, ST1966, ST1967, and ST1968 [16,35,36]. However, it has been shown that there is a specific subgroup of human ST398 strains, different from LA-MRSA ST398 [37], that can be readily differentiated by SNP detection and the presence or absence of scn and tet(M) [38]. Research in the Netherlands revealed that all the CC398 strains there typically are LA-MRSA [39].…”

Section: Cc398mentioning

confidence: 99%

Livestock-Associated MRSA and Its Current Evolution

Butaye

Argudín

Smith

2016

Curr Clin Micro Rpt

116

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a relatively recent phenomenon in veterinary medicine. Although in the beginning it was restricted to a single clonal complex (CC), CC398, it has expanded into several clonal complexes, and the diversity of subtypes in the clonal complexes is increasing also. The prevalence of each type is determined somewhat geographically; for instance, the most prevalent clonal complex in Europe is CC398, whereas in Asia, it is CC9. Although few data exist regarding North America, the situation appears to be mixed there. The SCCmec cassettes detected in LA-MRSA are limited mainly to SCCmec IVa and SCCmec V, although non-typeable cassettes and SCCmec type XI, containing mecC, also have been found.The source of the SCCmec in LA-MRSA was discovered to be animals. In searching from which bacteria the SCCmec cassettes in LA-MRSA have been transferred, the most obvious species to consider are the methicillin-resistant non-S. aureus staphylococci (MRNaS). However, very few data are available from those species in animals, and the data that do exist are not detailed enough to determine the origin. Nevertheless, similar cassettes were found in MRNaS, indicating a possible origin that needs to be investigated further.

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Section: Cc398mentioning

confidence: 99%

Livestock-Associated MRSA and Its Current Evolution

Butaye

Argudín

Smith

2016

Curr Clin Micro Rpt

116

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“…Jego obecność stwierdza się prawie u wszystkich profagów φ3. Gen chp kodujący białko hamujące chemotaksję (CHIPS, chemotaxis inhibitory protein) występuje u około 50% profagów (47). Ponadto około 75% profagów zawiera gen sak odpowiedzialny za wytwarzanie stafylokinazy oraz u połowy fagów wykrywa się geny odpowiedzialne za wytwarzanie enterotoksyn typu A oraz typu P, o wła-ściwościach superantygenów (25).…”

Section: Struktura Genomu Staphylococcus Aureusunclassified

“…Szczepy uznane za typowo "ludzkie" określono jako CC398-I/II-GOI, natomiast związane ze zwierzętami jako CC398-IIa (47). Obie linie można odróżnić na podstawie wyników analizy polimorfizmu pojedynczego nukleotydu.…”

Section: Struktura Genomu Staphylococcus Aureusunclassified

“…Do tej pory gen ten stwierdzano jedynie u szczepów izolowanych od zwierząt. Natomiast u szczepów linii CC398-I/II-GOI zazwyczaj wykrywa się obecność genu scn oraz czasami gen leukocydyny Panton-Valentine (47). Analiza polimorfizmu pojedynczego nukleotydu jest metodą kosztowną, dlatego do różnicowania gronkowców z kompleksu klonalnego 398 pochodzących od ludzi i zwierząt zaproponowano wykrywanie genów z grupy IEC, obecności profaga φ3 oraz genu tet(M).…”

Section: Struktura Genomu Staphylococcus Aureusunclassified

“…Badając w ten sposób szczepy wyizolowane od nosicieli niestykających się ze zwierzętami wykazano, że wszystkie należały do linii "zwierzęcej" (33). Z uwagi na ciągłe zmiany genotypowe wśród S. aureus nie można wykluczyć procesu readaptacji szczepów LA-MRSA związanych ze zwierzętami do ludzi (47).…”

Section: Struktura Genomu Staphylococcus Aureusunclassified

See 2 more Smart Citations

Livestock-associated strains of methicillin resistant Staphylococcus aureus (LA-MRSA) – the current state of knowledge

Kizerwetter-Świda¹,

Pławińska-Czarnak²

2017

Medycyna Weterynaryjna

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A methicillin-resistant Staphylococcus aureus (MRSA) referred to as livestock-associated (LA-MRSA) has recently emerged in farm animals, particularly in pigs. Strains of this MRSA variant from Europe and North America mostly belong to clonal complex (CC) 398. Generally LA-MRSA cause asymptomatic colonization among pigs, but also in veal calves, broiler chickens, turkeys, and horses. People in contact with livestock animals are at high risk of asymptomatic colonization or infection with these bacteria. In previous years, the impact of LA-MRSA on human health was considered small. However, LA-MRSA has become more prevalent among people without direct livestock contact, especially in areas with a high density of pig production. As a result of horizontal gene transfer S. aureus CC398 strains are constantly evolving. The adaptive power of S. aureus to new hosts and acquisition of resistance to antibiotics may cause the emergence of new, more virulent clones. LA-MRSA has evolved from human-adapted methicillin-susceptible S. aureus CC398, which was proved by comparative genome analysis. The adaptation to livestock was associated with several genetic changes. The most worrying aspect of MRSA CC398 seems to be its ability to spread to humans. For this reason, continuous surveillance of further genetic changes is recommended.

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A Survey of Chinese Pig Farms and Human Healthcare Isolates Reveals Separate Human and Animal Methicillin‐Resistant Staphylococcus aureus Populations

et al. 2021

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There has been increasing concern that the overuse of antibiotics in livestock farming is contributing to the burden of antimicrobial resistance in people. Farmed animals inEurope and North America, particularly pigs, provide a reservoir for livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA ST398 lineage) found in people. This study is designed to investigate the contribution of MRSA from Chinese pig farms to human infection. A collection of 483 MRSA are isolated from 55 farms and 4 hospitals in central China, a high pig farming density area. CC9 MRSA accounts for 97.2% of all farm isolates, but is not present in hospital isolates. ST398 isolates are found on farms and hospitals, but none of them formed part of the "LA-MRSA ST398 lineage" present in Europe and North America. The hospital ST398 MRSA isolate form a clade that is clearly separate from the farm ST398 isolates. Despite the presence of high levels of MRSA found on Chinese pig farms, the authors find no evidence of them spilling over to the human population. Nevertheless, the ST398 MRSA obtained from hospitals appear to be part of a widely distributed lineage in China. The new animal-adapted ST398 lineage that has emerged in China is of concern.

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Rapid Differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades

Cited by 84 publications

References 29 publications

Livestock-Associated MRSA and Its Current Evolution

Livestock-Associated MRSA and Its Current Evolution

Livestock-associated strains of methicillin resistant Staphylococcus aureus (LA-MRSA) – the current state of knowledge

A Survey of Chinese Pig Farms and Human Healthcare Isolates Reveals Separate Human and Animal Methicillin‐Resistant Staphylococcus aureus Populations

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