Rapid Differential Diagnosis of Seven Human Respiratory Coronaviruses Based on Centrifugal Microfluidic Nucleic Acid Assay

Xiong, Huiwen; Ye, Xin; Li, Yang; Wang, Limin; Zhang, Jin; Fang, Xueen; Kong, Jilie

doi:10.1021/acs.analchem.0c03364

Cited by 40 publications

(25 citation statements)

References 25 publications

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“…To facilitate comprehensive applications, integration of the current method with microfluidic system would be necessary because in many cases there is a need for diagnostic technologies to be able to simultaneously test many targets. Xiong et al reported a centrifugal microfluidic system, which could detect 32 targets in a single chip 29 . By combining with the microfluidic system, the capability of our method could be further expanded for the detection of multiple infections, such as detection of HR-HPVs together with other vaginal pathogens in a single assay.…”

Section: Resultsmentioning

confidence: 99%

A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology

Gong

Zhang²,

Wang

et al. 2021

Sci Rep

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Cervical carcinoma is the second most common cancer in women worldwide with greater than 99% of the cases caused by human papillomaviruses (HPVs). Early detection of HPVs especially the high risk types (HR-HPVs) are essential to prevent the disease progression. The existing methods for HPV detection, such as qPCR are of high sensitivity and specificity, but the need for expensive machinery and well-trained personnel slow down the disease detection. The emerging Cas12a-based method presents a new technique for nucleic acid detection. However, it is time-consuming and labor-intensive when used for HPV detection, as several reactions are required in order to identify multiple HPV infections. We herein present a non-genotyping method for 13 types of HR-HPV detection in a single reaction by combining the isothermal recombinase polymerase amplification (RPA) method with CRISPR-Cas12a technology. The result could be achieved in 35 min with high sensitivity (500 copies per reaction). This assay represents great advances for the application of RPA-Cas12a system and holds a great potential to address the key challenges facing the HPV diagnostics.

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Section: Resultsmentioning

confidence: 99%

A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology

Gong

Zhang²,

Wang

et al. 2021

Sci Rep

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“…In another example, The simultaneous detection of seven coronaviruses (SARS‐CoV, MERS‐CoV, SARS‐CoV‐2, and HCoV‐229E, HCoVOC43, HCoV‐NL63, and HCoV‐HKU1) was realized by using a lab‐on‐a‐CD via LAMP within 40 min. [ 184 ] More interestingly, the same group [ 185 ] developed a small‐volume rotating microfluidics for air sampling and detecting aerosol SARS‐CoV‑2, demonstrating LOD of 10 copies µL −1 in 15 min. Moreover, these lab‐independent readout systems enhanced the portability of microfluidic chip as a POCT strategy.…”

Section: Pocts For Diagnosis Of Coronaviruses (Sars Mers and Covid‐19)mentioning

confidence: 99%

Advanced Point‐of‐Care Testing Technologies for Human Acute Respiratory Virus Detection

et al. 2021

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system leading to respiratory tract infections (respiratory viruses, RVs). These diseases have the highest morbidity and fatality rates among acute infectious diseases and have caused a series of pandemics throughout human history. The first recorded RV occurred in 1200 BC, and subsequent major RVs have included the 1918 influenza virus, [1] severe acute respiratory syndrome coronavirus (SARS-CoV-1), [2][3][4][5] 2009 swine flu (H1N1), [6] Middle East respiratory syndrome (MERS) coronavirus, [7,8] and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), [9,10] which together have cost billions of lives (Figure 1). [11,12] The epidemics mentioned above impose a significant burden on healthcare systems and the global economy. [13,14] The recurrence of RV epidemics could result from their ubiquitous transmission routes, including direct transmission by sneezing, airborne/aerosol transmission by droplets from infected persons, and transmission by hand-to-eye/nose/mouth contact spreading RVs potentially from contaminated surfaces (handles, elevators, commonly used shared areas, and shopping bags) to an uninfected person. [15][16][17] The major RV types are influenza virus, coronavirus (CoV), adenovirus, respiratory syncytial virus (RSV), rhinovirus,The ever-growing global threats to human life caused by the human acute respiratory virus (RV) infections have cost billions of lives, created a significant economic burden, and shaped society for centuries. The timely response to emerging RVs could save human lives and reduce the medical care burden. The development of RV detection technologies is essential for potentially preventing RV pandemic and epidemics. However, commonly used detection technologies lack sensitivity, specificity, and speed, thus often failing to provide the rapid turnaround times. To address this problem, new technologies are devised to address the performance inadequacies of the traditional methods. These emerging technologies offer improvements in convenience, speed, flexibility, and portability of point-of-care test (POCT). Herein, recent developments in POCT are comprehensively reviewed for eight typical acute respiratory viruses. This review discusses the challenges and opportunities of various recognition and detection strategies and discusses these according to their detection principles, including nucleic acid amplification, optical POCT, electrochemistry, lateral flow assays, microfluidics, enzyme-linked immunosorbent assays, and microarrays. The importance of limits of detection, throughput, portability, and specificity when testing clinical samples in resource-limited settings is emphasized. Finally, the evaluation of commercial POCT kits for both essential RV diagnosis and clinical-oriented practices is included.

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“…The multi-respiratory virus screening microfluidic chip provides a key strategy for the future development of further respiratory virus detection methods. Recently, a portable microfluidic system that simultaneously diagnoses seven human coronaviruses was developed in response to the current outbreak of SARS-CoV-2 [56]. This chip, which is based on the LAMP method, has the ability to simultaneously detect SARS-CoV, MERS-CoV, SARS-CoV-2 and four other human coronaviruses (HCoV) (i.e., HCoV-OC43, HCoV-229E, HCoV-HKU1 and HCoV-NL63) within 40 min.…”

Section: Microfluidic Device For Respiratory Virus Detectionmentioning

confidence: 99%

Methods of Respiratory Virus Detection: Advances towards Point-of-Care for Early Intervention

Lin

Zhang

et al. 2021

Micromachines

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Respiratory viral infections threaten human life and inflict an enormous healthcare burden worldwide. Frequent monitoring of viral antibodies and viral load can effectively help to control the spread of the virus and make timely interventions. However, current methods for detecting viral load require dedicated personnel and are time-consuming. Additionally, COVID-19 detection is generally relied on an automated PCR analyzer, which is highly instrument-dependent and expensive. As such, emerging technologies in the development of respiratory viral load assays for point-of-care (POC) testing are urgently needed for viral screening. Recent advances in loop-mediated isothermal amplification (LAMP), biosensors, nanotechnology-based paper strips and microfluidics offer new strategies to develop a rapid, low-cost, and user-friendly respiratory viral monitoring platform. In this review, we summarized the traditional methods in respiratory virus detection and present the state-of-art technologies in the monitoring of respiratory virus at POC.

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Rapid Differential Diagnosis of Seven Human Respiratory Coronaviruses Based on Centrifugal Microfluidic Nucleic Acid Assay

Cited by 40 publications

References 25 publications

A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology

A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology

Advanced Point‐of‐Care Testing Technologies for Human Acute Respiratory Virus Detection

Methods of Respiratory Virus Detection: Advances towards Point-of-Care for Early Intervention

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