Rapid Diagnostic Platform for Colorimetric Differential Detection of Dengue and Chikungunya Viral Infections

Wang, Rui-Sheng; Ongagna-Yhombi, Serge Y.; Lu, Zhengda; Centeno‐Tablante, Elizabeth; Colt, Susannah; Cao, Xiangkun Elvis; Ren, Yue; Cárdenas, Washington B.; Mehta, Saurabh; Erickson, David

doi:10.1021/acs.analchem.9b00704

Cited by 35 publications

(32 citation statements)

References 37 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sample types included whole blood, plasma, and serum. Twelve studies examined the performance of antibody-based RDTs [11][12][13][14][15][16][17][9,18][19][9] while five the antigen-based RDTs [20][21][22][5][23].…”

Section: Resultsmentioning

confidence: 99%

Mapping The Global Landscape Of Chikungunya Rapid Diagnostic Tests: A Scoping Review

Moreira

Brasil

Dittrich

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Chikungunya virus (CHIKV) is a re-emerging arboviral disease and represents a global health threat because of the unprecedented magnitude of its spread. Diagnostics strategies rely heavily on reverse transcriptase-polymerase chain reaction (RT-PCR) and antibody detection by Enzyme-linked Immunosorbent assay (ELISA). Rapid Diagnostic Tests (RDTs) are available and promise to decentralize testing and increase availability at lower healthcare system levels. Objectives We aim to identify the extent of research on CHIKV RDTs, map the global availability of CHIKV RDTs, and evaluate the accuracy of CHIKV RDTs for the diagnosis of CHIKV. Eligibility criteria We included studies reporting symptomatic individuals suspected of CHIKV, tested with CHIKV RDTs, against the comparator being a validated laboratory-based RT-PCR or ELISA assay. The primary outcome was the accuracy of the CHIKV RDT when compared with reference assays. Sources of evidence Medline, EMBASE, and Scopus were searched from inception to October 13th , 2021. National regulatory agencies (European Medicines Agency, U.S. Food and Drug Administration, and the Brazilian National Health Surveillance Agency) were also searched for registered CHIKV RDTs. Results Eighteen studies were included and corresponded to 3722 samples tested with RDTs between 2005-2019. The most development stage of CHIKV RDTs studies was phase I (7/18 studies) and II (7/18 studies). No studies were in phase IV. The countries that manufacturer the most CHIKV RDTs were Brazil (n=17), followed by the USA (n=7), and India (n=6). Neither at EMA nor FDA, registered products were found. Conversely, the ANVISA has approved 23 CHIKV RDTs. Antibody RDTs (n=43) predominated and demonstrated sensitivity between 20% and 100%. The sensitivity of the antigen RDTs ranged from 33.3 to 100%. Conclusions The landscape of CHIKV RDTs is fragmented and needs coordinated efforts to ensure that patients in CHIKV-endemic areas have access to appropriate RDTs. Further research is crucial to determine the impact of such tests on integrated fever case management and prescription practices for acute febrile patients.

show abstract

Section: Resultsmentioning

confidence: 99%

Mapping The Global Landscape Of Chikungunya Rapid Diagnostic Tests: A Scoping Review

Moreira

Brasil

Dittrich

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Co-infections of different malaria species, though uncommon, need to be further examined to distinguish from the single infection of P. falciparum . For the practical use in peripheral settings, the image analysis algorithm can be implemented in the image reader [21, 22]. It is envisioned that the two-colour LFA can be further extended to a three-colour system by incorporating red, blue and green conjugations together, and this offers the possibility to detect 6 different analytes with two test lines on the strip.…”

Section: Resultsmentioning

confidence: 99%

A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line

Kim

Cao

Finkelstein

et al. 2019

Malar J

Self Cite

View full text Add to dashboard Cite

Background Malaria continues to impose a tremendous burden in terms of global morbidity and mortality, yet even today, a large number of diagnoses are presumptive resulting in lack of or inappropriate treatment. Methods In this work, a two-colour lateral flow immunoassay (LFA) system was developed to identify infections by Plasmodium spp. and differentiate Plasmodium falciparum infection from the other three human malaria species (Plasmodium vivax, Plasmodium ovale, Plasmodium malariae). To achieve this goal, red and blue colours were encoded to two markers on a single test line of strips, for simultaneous detection of PfHRP2 (red), a marker specific for P. falciparum infection, and pLDH (blue), a pan-specific marker for infections by all species of Plasmodium. The assay performance was first optimized and evaluated with recombinant malarial proteins spiked in washing buffer at various concentrations from 0 to 1000 ng mL−1. The colour profiles developed on the single test line were discriminated and quantified: colour types corresponded to malaria protein species; colour intensities represented protein concentration levels. Results The limit of detection (the lowest concentrations of malaria antigens that can be distinguished from blank samples) and the limit of colour discrimination (the limit to differentiate pLDH from PfHRP2) were defined for the two-colour assay from the spiked buffer test, and the two limits were 31.2 ng mL−1 and 7.8 ng mL−1, respectively. To further validate the efficacy of the assay, 25 human whole blood frozen samples were tested and successfully validated against ELISA and microscopy results: 15 samples showed malaria negative; 5 samples showed P. falciparum positive; 5 samples showed P. falciparum negative, but contained other malaria species. Conclusions The assay provides a simple method to quickly identify and differentiate infection by different malarial parasites at the point-of-need and overcome the physical limitations of traditional LFAs, improving the multiplexing potential for simultaneous detection of various biomarkers.

show abstract

“…With the use of an optical reader, the colorimetric tests can provide quantitative measurements based on a strong correlation between concentration of analyte and corresponding color intensity (refer to Figure 1 ). 82 In this case, the optical reader eliminates the subjective interpretation of the test results and offers a semiquantitative or even quantitative readout. The development of hybrid substrates (eg, agarose) can offer appropriate control over fluid flow for the optimal interaction between biomolecules and gold nanoparticle-modified test strips.…”

Section: Point-of-care Diagnostics Of Major Infectious Diseasesmentioning

confidence: 99%

Aspects of Point-of-Care Diagnostics for Personalized Health Wellness

Kumar

Nehra

Khurana

et al. 2021

IJN

View full text Add to dashboard Cite

Rapid Diagnostic Platform for Colorimetric Differential Detection of Dengue and Chikungunya Viral Infections

Cited by 35 publications

References 37 publications

Mapping The Global Landscape Of Chikungunya Rapid Diagnostic Tests: A Scoping Review

Mapping The Global Landscape Of Chikungunya Rapid Diagnostic Tests: A Scoping Review

A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line

Aspects of Point-of-Care Diagnostics for Personalized Health Wellness

Contact Info

Product

Resources

About