Rapid diagnosis of tuberculosis directly from clinical specimens using a gene chip

Chang, Hao‐Ming; Huang, Ming-Shyan; Yeh, Ching-Sheng; Chen, C.C.; Yang, Mei; Sun, Chongyun; Lee, C.K.; Lin, Shiu‐Ru

doi:10.1111/j.1469-0691.2009.03045.x

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…[10][11][12] A variety of microfluidic devices have been fabricated based on this microfabrication process, such as micropumps, 13 microvalves, 14 microseparators, 15 microconcentrators, 16 and micromixers. 17 Furthermore, these PDMS-based microfluidic devices can even be integrated to perform a specific assay for various applications such as molecular diagnosis, 18 protein analysis, 19 and cell studies. 20 However, there are still potential limitations for PDMS-based microfluidic systems.…”

Section: A Microfluidicsmentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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Microfluidic techniques have been recently developed for cell-based assays. In microfluidic systems, the objective is for these microenvironments to mimic in vivo surroundings. With advantageous characteristics such as optical transparency and the capability for automating protocols, different types of cells can be cultured, screened, and monitored in real time to systematically investigate their morphology and functions under well-controlled microenvironments in response to various stimuli. Recently, the study of stem cells using microfluidic platforms has attracted considerable interest. Even though stem cells have been studied extensively using bench-top systems, an understanding of their behavior in in vivo-like microenvironments which stimulate cell proliferation and differentiation is still lacking. In this paper, recent cell studies using microfluidic systems are first introduced. The various miniature systems for cell culture, sorting and isolation, and stimulation are then systematically reviewed. The main focus of this review is on papers published in recent years studying stem cells by using microfluidic technology. This review aims to provide experts in microfluidics an overview of various microfluidic systems for stem cell research.

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Section: A Microfluidicsmentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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“…The advent of molecular biology techniques that led to significant advances in DNA amplification and hybridization techniques has significantly aided the rectification of current flaws in the diagnosis of cutaneous mycobacterial infections ( Jagielski et al., 2016 ). DNA sequencing, PCR-restriction fragment length polymorphism (RFLP) assays ( Wong et al., 2003 ), gene chip assays ( Chang et al., 2010 ), and commercial kits, such as GenoType and INNO-LiPA, are established nucleic acid-based assays for identification of mycobacterial infections ( Zhu et al., 2010 ). DNA sequencing, especially metagenomic next-generation sequencing (mNGS) ( Kong et al., 2022 ), has greater efficiency, sensitivity, and specificity in detection of pathogenic bacteria, and can even detect rare pathogens.…”

Section: Discussionmentioning

confidence: 99%

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

Wang

Gao

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionThe clinical practicability of DNA microarray chip in detecting the presence of mycobacterial species/isolates directly in the skin tissues has not been evaluated, nor the efficacy of DNA microarray chip as a novel diagnostic tool for the early diagnosis of cutaneous mycobacterial infections is known.MethodsThe present study analyzed the incidence of cutaneous mycobacterial infections in Shanghai and explored the efficacy of a novel DNA microarray chip assay for the clinical diagnosis of the disease from skin tissue specimens compared to traditional detection methods. A total of 60 participants fulfilling the defined diagnostic criteria and confirmed positive for cutaneous mycobacterial infections from 2019 to 2021 were enrolled in the study. Subsequent to recording the participants’ medical history and clinical characteristics, the skin tissue specimens were collected for analyses. The specimens underwent histopathological analyses, skin tissue culture, and DNA microarray chip assay.ResultsIncreased incidence of cutaneous mycobacterial infection was detected from 2019 to 2021. The most common infecting pathogen was M. marinum followed by M. abscessus. The sensitivity, specificity and accuracy of the skin tissue culture method were 70%, 100% and 76.62%, respectively, while that of the DNA microarray chip assay were 91.67%, 100% and 93.51%, respectively. The sensitivity and accuracy of the DNA microarray chip assay were significantly higher than those of the skin tissue culture method. The positive likelihood and diagnostic odds ratio were >10 and >1, respectively for both the methods. The negative likelihood ratio was significantly higher (30% vs 8.33%) and the Youden’s index was significantly lower (70.00% vs 91.67%) in the skin culture method compared to that of the DNA microarray chip assay. There was a significant association of false negative results with a history of antibiotic use in the skin tissue culture method.DiscussionGiven the increasing incidence of cutaneous mycobacterial infections, early diagnosis remains a prime clinical focus. The DNA microarray chip assay provides a simple, rapid, high-throughput, and reliable method for the diagnosis of cutaneous mycobacterial infections with potential for clinical application.

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“…Previously, the instrumentation and microarray components had been independently evaluated for detection of other infectious entities, such as staphylococcal isolates [21]. Recently, the technology of microarray had been implemented in identification of MTB complex [22], [23], which gave rapid and accurate detection of MTB complex. Meanwhile, microarray had been used in drug resistance TB finding in near years by [14], [24].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Biochip System in Determining Isoniazid and Rifampicin Resistances of Mycobacterium Tuberculosis in Sputum Samples

et al. 2012

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ObjectiveTo evaluate a biochip system in determining isoniazid and rifampicin resistances of Mycobacterium tuberculosis in sputum samples in a Chinese population.MethodsWe assembled 907 sputum smeared positive specimens of tuberculosis patients in total. Each sample would be separated into two parts for culture and biochip assay simultaneously. And those cultured positive and having full drug resistance results would be used as reference. The McNemar χ2 test was adopted for evaluating the paired 2×2 table.ResultsCompared with drug sensitivity test, the agreement rates of the two methods in detecting rifampicin and isoniazid resistances were 93.37% and 94.49%, respectively. The sensitivity and specificity of biochip in detecting isoniazid were 74.31% and 96.92%, respectively. Meanwhile, the sensitivity and specificity for rifampicin were 79.76% and 96.53%, respectively. For multi-drug resistance, the sensitivity and specificity were 64.62% and 97.75%, respectively.ConclusionsThe biochip system is a rapid and accurate method for drug resistant tuberculosis diagnosis using sputum samples directly, especially for rifampicin resistance detection.

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Rapid diagnosis of tuberculosis directly from clinical specimens using a gene chip

Cited by 9 publications

References 24 publications

Stem cells in microfluidics

Stem cells in microfluidics

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

Evaluation of Biochip System in Determining Isoniazid and Rifampicin Resistances of Mycobacterium Tuberculosis in Sputum Samples

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