Rapid Diagnosis of Tick-Borne Illnesses by Use of One-Step Isothermal Nucleic Acid Amplification and Bio-Optical Sensor Detection

Kim, Ji Yeun; Koo, Bon San; Jin, Choong Eun; Kim, Min Chul; Chong, Yong Pil; Lee, Sang‐Oh; Choi, Sang Ho; Kim, Yang Soo; Woo, Jun Hee; Shin, Yong Moon; Kim, Sung-Han

doi:10.1373/clinchem.2017.280230

Cited by 16 publications

(22 citation statements)

References 34 publications

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“…In this work, we present a highly sensitive silicon microring resonator (SMR) bio-optical sensor based on isothermal nucleic acid amplification for the label-free detection of infectious agents using blood plasma specimens. Their operation is based on the change of the refractive index to the measurable spectral shift of the optical transmission, and they enable a real-time, label-free detection by monitoring changes in resonant wavelengths generated by biomolecules such as pathogens, proteins, and nucleic acids coupled with sensor ligands present on the sensor surface [20,21,22,23,24,25]. Photothermal spectroscopy, which indirectly measures the optical absorption of a material, allows measurements that are sensitive to changes in external conditions due to absorption only, unlike conventional methods of measuring the scattering and return loss [26].…”

Section: Introductionmentioning

confidence: 99%

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

et al. 2019

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Blood plasma from patients is a powerful resource for diagnosing infectious disease due to it having many genetic materials as well as being relatively easy to obtain. Thus, various biosensors have been investigated for diagnosing diseases in blood plasma. However, there are no optimized and validated sensors for clinical use due to the low sensitivity, complexity, and difficulties of removing the inhibitors from plasma samples. In this study, we described a silicon microring resonator sensor used to detect Coxiella burnetii from the blood plasma of Q-fever patients in a label-free, real-time manner. Q-fever is an infectious disease caused by Coxiella burnetii via direct contact or inhalation aerosols. We validated this biosensor in the blood plasma of 35 clinical samples (including 16 Q fever samples infected with Coxiella burnetii and 19 samples infected with other febrile diseases. The biosensors are capable of rapid (10 min), highly sensitive (87.5%), and specific (89.5%) detection in plasma samples compared to the use of the conventional method.

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Section: Introductionmentioning

confidence: 99%

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

et al. 2019

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“…1 shows the design of the arch-shaped multiple-target sensing platform for rapid diagnosis of emerging infectious pathogens, such as MERS-CoV, HCoV, ZIKV, and EBOV, using a SMR sensor. We established a series of primers for target amplification and simultaneous detection of specific multiple-targets rather than asymmetric amplification on a SMR sensor lacking multiple target detection (Kim et al, 2018;Koo et al, 2017). For multiple-target sensing, the SMR surface was first amine-modified with APTES and linked to the NH 2 -modified 5′-end of both primers.…”

Section: Resultsmentioning

confidence: 99%

“…To use the arch-shaped multiple-target sensing platform as a multiple detection system, we structured the SMR sensor as previously described (Kim et al, 2018;Koo et al, 2017). For arch-shaped amplification and detection with specific primers immobilized on the sensor, a three-step primer modification was required on the surface of the sensing chip.…”

Section: Development and Operation Of The Arch-shaped Multiple-targetmentioning

confidence: 99%

“…In this study, rather than applying the amplified target and probe hybridization assay, we established long (> 50 base pair, bp) oligonucleotide primers at a high concentration (5 μM) to create an arch shape on the sensor surface for solid-phase amplification, thus overcoming primer dimerization. The SMR sensor allows for sensitive, label-free, and real-time sensing (Kim et al, 2018;Koo et al, 2017), and the long primers at a high concentration greatly increased the rate and detection sensitivity of this method. For nucleic acid amplification, we used a recombinase polymerase amplification (RPA) enzyme, which requires a small instrument for isothermal amplification (Piepenburg et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Arch-shaped multiple-target sensing for rapid diagnosis and identification of emerging infectious pathogens

Koo

Hong

Jin

et al. 2018

Biosensors and Bioelectronics

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Rapid identification of emerging infectious pathogens is crucial for preventing public health threats. Various pathogen detection techniques have been introduced; however, most techniques are time-consuming and lack multiple-target detection specificity. Although multiple-target detection techniques can distinguish emerging infectious pathogens from related pathogens, direct amplification methods have not been widely examined. Here, we present a novel arch-shaped multiple-target sensor capable of rapid pathogen identification using direct amplification in clinical samples. In this study, an arch-shaped amplification containing primer sequences was designed to rapidly amplify multiple targets. Further, the sensing platform allowed for sensitive and specific detection of human coronavirus, Middle East respiratory syndrome, Zika virus, and Ebola virus down to several copies. This platform also simultaneously distinguished between Middle East respiratory syndrome and human coronavirus in clinical specimens within 20 min. This arch-shaped multiple-target sensing assay can provide rapid, sensitive, and accurate diagnoses of emerging infectious diseases in clinical applications.

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“…Several molecular assays have been developed and are currently used for SFTS diagnosis, such as real-time reverse transcription (RT)-PCR and loop-mediated isothermal amplification combined with reverse transcription. The majority of molecular assays were dewww.annlabmed.org https://doi.org/10.3343/alm.2020.40.4.317 signed to target the L, M, or S segments of the SFTSV genome; of these, the S segment is known to be the most conserved gene [5][6][7][8][9][10].…”

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confidence: 99%

Comparison Between the SFTS-QS Kit and the PowerChek SFTSV Real-time PCR Kit for the Detection of Severe Fever With Thrombocytopenia Syndrome Virus

et al. 2020

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The recent increase in severe fever with thrombocytopenia syndrome (SFTS) cases has led to the development of the SFTS-QS kit (MiCoBioMed, Seongnam, Korea) for detecting the SFTS virus (SFTSV, now renamed Huaiyangshan banyangvirus). SFTS-QS is a qualitative real-time reverse transcription PCR assay based on lab-on-a-chip technology. We evaluated the performance of the SFTS-QS kit and compared it with that of the PowerChek SFTSV Real-time PCR kit (PowerChek; Kogene Biotech, Seoul, Korea). A total of 117 serum samples were simultaneously assayed using the SFTS-QS and PowerChek kits. Sanger sequencing targeting the S and M segments of SFTSV was performed as the reference method. The total turnaround time of the two kits was compared. The SFTS-QS results agreed with those of PowerChek with a kappa value of 0.92. The diagnostic sensitivity and specificity of the SFTS-QS kit were both 100% (14/14 and 103/103, respectively), whereas those of the PowerChek kit were 100% (14/14) and 98.1% (101/103), respectively. The results of SFTS-QS and PowerChek were comparable; however, the SFTS-QS kit required a shorter total turnaround time. The SFTS-QS kit produced accurate and fast results and thus could serve as a useful tool for detecting SFTSV. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Rapid Diagnosis of Tick-Borne Illnesses by Use of One-Step Isothermal Nucleic Acid Amplification and Bio-Optical Sensor Detection

Abstract: iNAD is a valuable, rapid method of detecting SFTS virus and with high clinical sensitivity and specificity.

Cited by 16 publications

References 34 publications

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

Arch-shaped multiple-target sensing for rapid diagnosis and identification of emerging infectious pathogens

Comparison Between the SFTS-QS Kit and the PowerChek SFTSV Real-time PCR Kit for the Detection of Severe Fever With Thrombocytopenia Syndrome Virus

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