Rapid diagnosis of liver fibrosis using multimodal multiphoton nonlinear optical microspectroscopy imaging

Abstract

Abstract. A multimodal multiphoton nonlinear optical (NLO) microspectroscopy imaging system was developed using a femtosecond laser and a photonic crystal fiber. Coherent anti-Stokes Raman scattering (CARS) microspectroscopy was combined with two-photon excitation fluorescence and second-harmonic generation microscopy in one platform and the system was applied to diagnose liver fibrosis. Normal and liver fibrosis tissues were clearly distinguished with the great difference from CARS spectra as well as multimodal multiphoton NLO images. We expect the system to be a rapid diagnosis tool for liver fibrosis at tissue level with label-free imaging of significant biochemical components. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Automated classification of hepatocellular carcinoma differentiation using multiphoton microscopy and deep learning

“…Two of the most well‐used nonlinear optical effects are as follows: (a) second‐harmonic generation (SHG), which can visualize collagen without the need for any exogenous dyes and (b) two‐photon excitation fluorescence (TPEF), which can reveal the morphology of hepatocytes. Because these imaging modalities (TPEF and SHG) can provide in tandem complementary information about tissue morphology and organization, the combination of TPEF and SHG may be used to monitor liver disease .…”

Determination of degree of unsaturation in edible oils using coherent anti‐Stokes Raman scattering spectroscopy

“…In this paper, we performed femtosecond CARS spectroscopy in six commercial oils and five FAs and showed that the IV can be obtained from the CARS intensity ratio (I C–H /I C–H ) of specific two C–H stretch bands (C–H, –C–H). An ultra‐short CARS setup employing a femtosecond laser and a photonics crystal fiber was used to minimize the nonresonant signal . From the experiment, we observed the relation between the CARS intensity ratio and mass unsaturation of the oils and found the calibration curve for estimating IV of edible oils.…”

Label-free imaging and quantitative chemical analysis of Alzheimer’s disease brain samples with multimodal multiphoton nonlinear optical microspectroscopy

“…Our laser system is described in greater detail in our previous paper. 25 The laser beam passed through an optical isolator (IO-5-NIR-LP, Thorlabs) and was divided into two beams (3:1) by a beam splitter (BP133, Thorlabs). The reflected beam was transmitted via a series of optical components for spatial filtering, and a "pump beam" in a narrow band for the CARS process, with a pulse of 0.8 nm and center wavelength of 800 nm, was generated by combining two filters (LL01-808-12.5, Semrock and custom-made filter from Andover Corporation).…”

“…This multimodal multiphoton microendoscope imaging modality was modified and developed from a previously described system. [23][24][25] The laser system used a homemade Ti:sapphire femtosecond oscillator pumped by a green laser source (Verdi V5, Coherent). The output beam from the oscillator generates a 120 fs, 56.1 MHz pulse train, and a 460 mW average output power with a center wavelength of 800 nm.…”