Rapid diagnosis of Ditylenchus destructor by loop-mediated isothermal amplification assay based on 28S rRNA sequences

Diagnosis of potato rot nematode Ditylenchus destructor using PCR-RFLP

“…The amplification of ITS1-5.8S-ITS2 of the ribosomal DNA produced a fragment of almost 1000 bp. Nowadays, the molecular diagnostic of D. destructor is primarily based on the specific sequences of some conserved regions by the conventional PCR, and the most commonly used regions are small subunits and internal transcribed spacer (ITS) of ribosomal RNA [2].…”

“…ITS1 differed in length from 315 to 473 bp, 5.8S measured ca 154bp and ITS2 was 207 bp. The identified distinction in length in the ITS1 was affected by the insertion of 57 to 188 bp in the entire length in some D. destructor [2]. A mentioned primer set can be used for specific identification of D. destructor.…”

“…Nematodes are an important group of organisms having evident biological progress, characterized by the high amount of density growth, the broad spectrum of spreading, and having numerous intraspecific variable symptoms. They are listed among the quarantine pests in some European countries [1][2][3] and they were also evaluated as a serious pest in the globe [4]. Ditylenchus destructor could be a significant parasite in different host plants.…”

“…Without taxonomic expertise, the distinguishing of D. destructor from other species is difficult. There are some methods using PCR with specific primers designed based on nucleotides combination in the ITS rRNA gene which were also developed for the detection of D. destructor [2,5,[9][10][11]. Molecular identification of D. destructor is based on the specific sequences of rDNA region by the conventional PCR methods [8,12] and the most customarily used regions are the large subunits [13], small subunits and the internal transcribed spacer (ITS) of ribosomal RNA [14,15].…”

Chemical constituents from the aerial parts of Rhododendron thymifolium and its bioactivities against Tribolium castaneum, Lasioderma serricorne, and Ditylenchus destructor

“…[2] Ditylenchus destructor is an important plant pathogenic nematode in agricultural production and is listed as a quarantine pest by many countries and regions. [3] Currently, plant-parasitic nematodes are major pests responsible for global agricultural losses amounting to an estimated $157 billion annually. [4] The widespread using of synthetic pesticides has resulted in the development of insecticide-resistant populations and harmful effects on human health and the environment.…”