Rapid development of proteomics in China: from the perspective of the Human Liver Proteome Project and technology development

Li, Ning; Xu, Zhongwei; Zhai, Linhui; Li, YanChang; Fan, Fengxu; Zheng, Junjie; Xu, Ping; He, Fuchu

doi:10.1007/s11427-014-4714-2

Cited by 7 publications

(5 citation statements)

References 91 publications

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“…UC is a type of inflammatory bowel disease (IBD) characterized by chronic recurrent, and the specific aetiology and pathogenesis of UC are still not clear. Proteomics is a discipline that systematically quantifies all the proteins in cells or organisms and elucidates their biological functions, it has enormous potential for early diagnosis, prevention, and prognosis prediction of diseases[ 17 , 18 ]. In recent years, proteomics has gradually been applied to the study of UC, providing strong support for the research of pathogenesis, clinical diagnosis, and treatment of UC.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics

Qi¹,

Zhong²,

Liu³

et al. 2022

WJG

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BACKGROUND Ulcerative colitis (UC) is a chronic, nonspecific intestinal inflammatory disease. Acupuncture and moxibustion is proved effective in treating UC, but the mechanism has not been clarified. Proteomic technology has revealed a variety of biological markers related to immunity and inflammation in UC, which provide new insights and directions for the study of mechanism of acupuncture and moxibustion treatment of UC. AIM To investigate the mechanism of electroacupuncture (EA) and herb-partitioned moxibustion (HM) on UC rats by using proteomics technology. METHODS Male Sprague-Dawley rats were randomly divided into the normal (N) group, the dextran sulfate sodium (DSS)-induced UC model (M) group, the HM group, and the EA group. UC rat model was prepared with 3% DSS, and HM and EA interventions at the bilateral Tianshu and Qihai acupoints were performed in HM or EA group. Haematoxylin and eosin staining was used for morphological evaluation of colon tissues. Isotope-labeled relative and absolute quantification (iTRAQ) and liquid chromatography-tandem mass spectrometry were performed for proteome analysis of the colon tissues, followed by bioinformatics analysis and protein-protein interaction networks establishment of differentially expressed proteins (DEPs) between groups. Then western blot was used for verification of selected DEPs. RESULTS The macroscopic colon injury scores and histopathology scores in the HM and EA groups were significantly decreased compared to the rats in the M group ( P < 0.01). Compared with the N group, a total of 202 DEPs were identified in the M group, including 111 up-regulated proteins and 91 down-regulated proteins, of which 25 and 15 proteins were reversed after HM and EA interventions, respectively. The DEPs were involved in various biological processes such as biological regulation, immune system progression and in multiple pathways including natural killer cell mediated cytotoxicity, intestinal immune network for immunoglobulin A (IgA) production, and FcγR-mediated phagocytosis. The Kyoto Encyclopedia of Genes and Genomes pathways of DEPs between HM and M groups, EA and M groups both included immune-associated and oxidative phosphorylation. Network analysis revealed that multiple pathways for the DEPs of each group were involved in protein-protein interactions, and the expression of oxidative phosphorylation pathway-related proteins, including ATP synthase subunit g (ATP5L), ATP synthase beta subunit precursor (Atp5f), cytochrome c oxidase subunit 4 isoform 1 (Cox4i1) were down-regulated after HM and EA interventions. Subsequent verification of selected DEPs (Synaptic vesicle glycoprotein 2A; nuclear cap binding protein subunit 1; carbamoyl phosphate synthetase 1; Cox4i1; ATP synthase subunit b, Atp5f1; doublecortin like kinase 3) by western blot confirmed the reliability of the iTRAQ data, HM and EA interventions can significantly down-regulat...

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Section: Discussionmentioning

confidence: 99%

Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics

Qi¹,

Zhong²,

Liu³

et al. 2022

WJG

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“…21 The concept of proteomics was first proposed by Peter James in 1997, and International Human Protein Organization established in 2001 further promotes the development of proteomics. 22 Proteomics is used to qualitatively and quantitatively analyze all proteins in cells, tissues or organisms, and to reveal the protein interaction network and dynamic regulation process in complex biological systems at the protein level. 23 In addition to relying on mass spectrometry, the research of protein also depends on the protein database based on gene sequence.…”

Section: Origin and Development Of Omicsmentioning

confidence: 99%

Focus on the molecular mechanisms of cisplatin resistance based on multi-omics approaches

2023

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“…At the same time, it was the first time that Chinese team led international scientific research project. (CN)HLPP attracted more than 70 laboratories, and China made great progress in the proteome research . As an important component part in HPP, HLPP contributed information about human liver proteins in three aspects: expression and modification profiles; protein interaction and localization maps; sample, antibody, and data repositories.…”

Section: Proteomics Organization: From (Cn)hlpp To (Cn)hppmentioning

confidence: 99%

Proteomics and Precision Medicine

Chen

Qin

et al. 2019

Small Methods

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During the last decade, great progress has been made in mass spectrometry rendering the identification of 6000 proteins in an hour and up to 10 000 proteins in half a day. The proteome has been widely applied in a variety of studies in the life sciences, for dissecting protein profiling, protein–protein interactions, post‐translational‐modification, etc. Since the improvement of the proteome screening efficiency and the decreasing of the cost in sample measurement, the growing requirement of deep application of proteomics has been raised recently in clinical research, foretelling an era in which proteomics will enable personally tailored therapies for patients. This review describes the cutting‐edge progression of next‐generation proteomics and highlights its potential in precision medicine in the future.

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Rapid development of proteomics in China: from the perspective of the Human Liver Proteome Project and technology development

Cited by 7 publications

References 91 publications

Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics

Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics

Focus on the molecular mechanisms of cisplatin resistance based on multi-omics approaches

Proteomics and Precision Medicine

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