Rapid determination of Trypanosoma cruzi urinary antigens in human chronic chagas disease by agglutination test

Katzin, A. M.; Marcipar, A.; Freilij, Héctor; Corral, Ricardo S.; Yanovsky, J. F.

doi:10.1016/0014-4894(89)90099-4

Cited by 15 publications

(14 citation statements)

References 24 publications

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“…The presence of DNA in urine is associated with the presence of parasite DNA in blood and heart and with a high level of parasite DNA in blood, but not with the presence of parasites in kidney or kidney injury [189][190][191]. The detection of antigens within the urine of patients suffering from acute [192] or chronic CD [193] has opened up some new innovative approaches for diagnosis. A number of T. cruzi urinary antigens can be identified and classified according to their molecular weight, such as the 80 kDa iron-binding protein or the 150-160 kDa antigen.…”

Section: Urinementioning

confidence: 99%

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Sereno

Akhoundi

Sayehmri

et al. 2020

IJMS

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Unicellular eukaryotes of the Trypanosomatidae family include human and animal pathogens that belong to the Trypanosoma and Leishmania genera. Diagnosis of the diseases they cause requires the sampling of body fluids (e.g., blood, lymph, peritoneal fluid, cerebrospinal fluid) or organ biopsies (e.g., bone marrow, spleen), which are mostly obtained through invasive methods. Body fluids or appendages can be alternatives to these invasive biopsies but appropriateness remains poorly studied. To further address this question, we perform a systematic review on clues evidencing the presence of parasites, genetic material, antibodies, and antigens in body secretions, appendages, or the organs or proximal tissues that produce these materials. Paper selection was based on searches in PubMed, Web of Science, WorldWideScience, SciELO, Embase, and Google. The information of each selected article (n = 333) was classified into different sections and data were extracted from 77 papers. The presence of Trypanosomatidae parasites has been tracked in most of organs or proximal tissues that produce body secretions or appendages, in naturally or experimentally infected hosts. The meta-analysis highlights the paucity of studies on human African trypanosomiasis and an absence on animal trypanosomiasis. Among the collected data high heterogeneity in terms of the I 2 statistic (100%) is recorded. A high positivity is recorded for antibody and genetic material detection in urine of patients and dogs suffering leishmaniasis, and of antigens for leishmaniasis and Chagas disease. Data on conjunctival swabs can be analyzed with molecular methods solely for dogs suffering canine visceral leishmaniasis. Saliva and hair/bristles showed a pretty good positivity that support their potential to be used for leishmaniasis diagnosis. In conclusion, our study pinpoints significant gaps that need to be filled in order to properly address the interest of body secretion and hair or bristles for the diagnosis of infections caused by Leishmania and by other Trypanosomatidae parasites.

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Section: Urinementioning

confidence: 99%

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Sereno

Akhoundi

Sayehmri

et al. 2020

IJMS

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“…Soluble T. cruzi antigens with molecular weights of 150–160 kDa [15], 100 kDa [16], [17], 90–80 kDa [18], 80 kDa [16], [18]–[21], 70–65 kDa [18], 55–50 kDa [22], 55–45 kDa [18], 55 kDa [21], 50 kDa [17], and 40–35 kDa [18] have been reported in urine from animals and patients with Chagas disease. Although T. cruzi antigens in urine were presumed to derive from the systemic circulation [16], [17], amastigote nests have been demonstrated in kidney tissue of humans [23] and animals [24]–[26] and in the bladder of animals [27].…”

Section: Introductionmentioning

confidence: 99%

“…Although T. cruzi antigens in urine were presumed to derive from the systemic circulation [16], [17], amastigote nests have been demonstrated in kidney tissue of humans [23] and animals [24]–[26] and in the bladder of animals [27]. Although apparently rare, renal injury has been reported in human T. cruzi infection [28].…”

Section: Introductionmentioning

confidence: 99%

Detection of Soluble Antigen and DNA of Trypanosoma cruzi in Urine Is Independent of Renal Injury in the Guinea Pig Model

et al. 2013

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The diagnosis of Chagas disease in humans is generally limited to the detection of specific antibodies. Detection of T. cruzi antigens in urine has been reported previously, but is not used in the diagnosis. In this study, soluble T. cruzi antigens and DNA were detected in urine samples and were associated with kidney injury and systemic detection of the parasite. We used 72 guinea pigs infected with T. cruzi Y strain and 18 non-infected guinea pigs. Blood, kidney, heart and urine samples were collected during the acute phase and chronic phase. Urine samples were concentrated by ultrafiltration. Antigens were detected by Western Blot using a polyclonal antibody against trypomastigote excretory-secretory antigen (TESA). T. cruzi DNA was detected by PCR using primers 121/122 and TcZ1/TcZ2. Levels of T. cruzi DNA in blood, heart and kidney were determined by quantitative PCR. T. cruzi antigens (75 kDa, 80 kDa, 120 kDa, 150 kDa) were detected in the acute phase (67.5%) and the chronic phase (45%). Parasite DNA in urine was detected only in the acute phase (45%). Kidney injury was characterized by high levels of proteinuria, kidney injury molecule-1 (KIM-1) and urea, and some histopathological changes such as inflammation, necrosis, fibrosis and scarce parasites. The detection of antigens and DNA in urine was associated with the presence of parasite DNA in blood and heart and with high levels of parasite DNA in blood, but not with the presence of parasite in kidney or kidney injury. These results suggest that the detection of T. cruzi in urine could be improved to be a valuable method for the diagnosis of Chagas disease, particularly in congenital Chagas disease and in immunocompromised patients.

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“…To facilitate sample collection for immunodiagnosis, urine samples have been used to test for schistosomiasis, [8][9][10][11] malaria, 12 leishmaniasis, 13 Chagas disease, 14 and filariasis. [15][16][17] In human filariasis, the usefulness of immunoglobulin (Ig) G4 in the diagnosis of filarial infections has been reported.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples.

Itoh

Weerasooriya²,

Qiu³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. We developed an enzyme-linked immunosorbent assay (ELISA) that detects filaria-specific immunoglobulin G4 antibodies in unconcentrated urine. The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples collected in Thailand, Lao People's Democratic Republic, and Japan, where no human filariasis is known, 295 (99.0%) were negative by ELISA. Various intestinal nematode and fluke infections did not interfere with the ELISA. Urine samples with sodium azide could be kept at 37ЊC for 4 weeks, and the time of urine collection did not influence ELISA results. This ELISA can be used to identify endemic foci of filariasis.

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Rapid determination of Trypanosoma cruzi urinary antigens in human chronic chagas disease by agglutination test

Cited by 15 publications

References 24 publications

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Detection of Soluble Antigen and DNA of Trypanosoma cruzi in Urine Is Independent of Renal Injury in the Guinea Pig Model

Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples.

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