Rapid determination of the free and total hydroxytyrosol and tyrosol content in extra virgin olive oil by stable isotope dilution analysis and paper spray tandem mass spectrometry

Bartella, Lucia; Mazzotti, Fabio; Sindona, Giovanni; Donna, Leonardo Di

doi:10.1016/j.fct.2019.111110

Cited by 27 publications

(19 citation statements)

References 17 publications

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“…All of the data are reported in Table 2. These values are comparable with those obtained with other methods using LC-UV or DAD detectors or, in some cases, LC-MS, demonstrating the robustness of the method [22,35].…”

Section: Linear Rangesupporting

confidence: 82%

“…Simplified analytical protocols and confirmatory techniques are invoked to fulfill the lack of official methods for olive oil certification in terms of quality and nutritional properties [34]. Recently, paper spray tandem mass spectrometry was applied to quantify free and total Tyr and Htyr with good results in terms of reliability, rapidity, and sensitivity [35]. Alternatively, EVOO was characterized by the direct determination of the secoiridoids without the hydrolysis of the sample [36].…”

Section: Introductionmentioning

confidence: 99%

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Tyrosol and Hydroxytyrosol Determination in Extra Virgin Olive Oil with Direct Liquid Electron Ionization-Tandem Mass Spectrometry

et al. 2021

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Extra virgin olive oil (EVOO) is one of the main ingredients of the Mediterranean diet. It is claimed as a functional food for its unique content of health-promoting compounds. Tyrosol (Tyr), Hydroxytyrosol (Htyr), and their phenolic derivatives present in EVOO show beneficial properties, and their identification and quantification, both in their free form and after the hydrolysis of more complex precursors, are important to certify its quality. An alternative method for quantifying free and total Tyr and Htyr in EVOO is presented using an LC–MS interface based on electron ionization (EI), called liquid electron ionization (LEI). This method requires neither sample preparation nor chromatography; the sample is diluted and injected. The selectivity and sensitivity were assessed in multiple reaction monitoring mode (MRM), obtaining confirmation and quantification in actual samples ranging from 5 to 11 mg/Kg for the free forms and from 32 to 80 mg/Kg for their total amount after hydrolysis. Two MS/MS transitions were acquired for both compounds using the Q/q ratios as confirmatory parameters. Standard addition calibration curves demonstrated optimal linearity and negligible matrix effects, allowing a correct quantification even without expensive and difficult to find labeled internal standards. After several weeks of operation, the system’s repeatability was excellent, with an intraday RSD (%) spanning from five to nine and an interday RSD (%) spanning from 9 to 11.

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Section: Linear Rangesupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Tyrosol and Hydroxytyrosol Determination in Extra Virgin Olive Oil with Direct Liquid Electron Ionization-Tandem Mass Spectrometry

et al. 2021

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“…However, if manufacturers are claiming the presence of specific concentrations of antioxidants in their products, robust analytical methods are required to assess the validity of such claims. PSI‐MS was recently utilized in the measurement of tyrosol and hydroxytyrosol in olive oil, two antioxidants often reported to offer health benefits to consumers 92 . The authors demonstrated an accurate and sensitive technique for the rapid assessment of antioxidants in oil products, with the aim of upholding European regulations on food product health claims.…”

Section: Applications Of Ambient Ionization Mass Spectrometrymentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2020: An annual review

Rankin‐Turner

Heaney

2021

Analytical Science Advances

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Recent developments in mass spectrometry (MS) analyses have seen a concerted effort to reduce the complexity of analytical workflows through the simplification (or removal) of sample preparation and the shortening of run‐to‐run analysis times. Ambient ionization mass spectrometry (AIMS) is an exemplar MS‐based technology that has swiftly developed into a popular and powerful tool in analytical science. This increase in interest and demonstrable applications is down to its capacity to enable the rapid analysis of a diverse range of samples, typically in their native state or following a minimalistic sample preparation approach. The field of AIMS is constantly improving and expanding, with developments of powerful and novel techniques, improvements to existing instrumentation, and exciting new applications added with each year that passes. This annual review provides an overview of applications of AIMS techniques over the past year (2020), with a particular focus on the application of AIMS in a number of key fields of research including biomedical sciences, forensics and security, food sciences, the environment, and chemical synthesis. Novel ambient ionization techniques are introduced, including picolitre pressure‐probe electrospray ionization and fiber spray ionization, in addition to modifications and improvements to existing techniques such as hand‐held devices for ease of use, and USB‐powered ion sources for on‐site analysis. In all, the information provided in this review supports the view that AIMS has become a leading approach in MS‐based analyses and that improvements to existing methods, alongside the development of novel approaches, will continue across the foreseeable future.

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“…For the olive oil, the oil samples were treated according to the procedure present in the literature [18,19]: 25 µL of a d 2 -hydroxytyrosol solution at 2000 mg/L was mixed to 2 g of sample. The resulting mixture was homogenized by ultra-turrax (IKA Works, Inc., Staufen, Germany) for 3 min, and then 1 g was extracted using a 1 mL solution of 0.1% HCOOH/EtOH 3:7 v/v.…”

Section: Sample Preparation For Mass Spectrometric Analysismentioning

confidence: 99%

Hydroxytyrosol-Fortified Foods Obtained by Supercritical Fluid Extraction of Olive Oil

et al. 2021

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A new original process has been set-up to obtain hydroxytyrosol-enriched food by means of CO2 supercritical fluid extraction. The process is based on the direct adsorption of hydroxytyrosol on selected foodstuff (e.g., flour, whole-wheat flour, and sugar) when placed in contact with olive oil (which is known to contain this phenol) under controlled temperature and pressure conditions of supercritical CO2. The transfer of the nutraceutical to the foodstuff was initially evaluated using a fortified olive oil model and was then applied to commercial olive oil and foodstuff. The yield of the hydroxytyrosol transfer was demonstrated to be quantitative. In order to prove the suitability of the final products, hydroxytyrosol-enriched flour was used to prepare a bread roll, which maintained the nutraceutical characteristics after the cooking. Finally, DPPH based experiments were performed to prove the radical scavenging activity of the functionalized foodstuff.

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Rapid determination of the free and total hydroxytyrosol and tyrosol content in extra virgin olive oil by stable isotope dilution analysis and paper spray tandem mass spectrometry

Cited by 27 publications

References 17 publications

Tyrosol and Hydroxytyrosol Determination in Extra Virgin Olive Oil with Direct Liquid Electron Ionization-Tandem Mass Spectrometry

Tyrosol and Hydroxytyrosol Determination in Extra Virgin Olive Oil with Direct Liquid Electron Ionization-Tandem Mass Spectrometry

Applications of ambient ionization mass spectrometry in 2020: An annual review

Hydroxytyrosol-Fortified Foods Obtained by Supercritical Fluid Extraction of Olive Oil

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