Rapid determination of tetracycline antibiotics in serum by reversed-phase high-performance liquid chromatography with fluorescence detection

Iwaki, Kazuo; Okumura, Norio; Yamazaki, Masakazu

doi:10.1016/0378-4347(93)80124-m

Cited by 27 publications

(13 citation statements)

References 10 publications

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“…Collective results, given in Table 6, indicate the high accuracy and precision of the present work as compared to those previously reported [3,6] depending on more complicated instrumentations or time-consuming pretreatment steps, while the combination of sensitivity, selectivity and simplicity of ionselective electrode potentiometry makes it an excellent and versatile technique.…”

Section: Determination Of Ctcclsupporting

confidence: 48%

“…New CTC-selective PVC membrane electrodes of both coated wire and conventional types based on (CTC) 3 PT or (CTC) 3 PM ion-pair associates are constructed and applied in potentiometric determination of CTC.Cl. The electrodes proved to be successful, providing a rapid, simple and have low cost potentiometric methods for the determination of CTC.Cl in pure solutions and in pharmaceutical preparations.…”

Section: Discussionmentioning

confidence: 99%

“…The optimum membrane compositions (w=w) for (CTC) 3 The solution containing the ion-pair associate, PVC and DBP dissolved in THF as given above was poured into a 7.5-cm Petri dish. The solvent was allowed to evaporate at room temperature whereupon a film of about 1.0 mm thickness was obtained.…”

Section: Membrane Compositionmentioning

confidence: 99%

“…Several methods have been reported for the determination of chlortetracycline hydrochloride, including spectrophotometry [1,2], high performance liquid chromatography [3,4], fluorimetry [5], room temperature phosphorescence [6], flow injection potentiometry [7], capillary electrophoresis [8], flow-injection chemiluminescence [9], and differential pulse polarography [10]. Electrodes selective for chlortetracycline of the conventional membrane type based on CTC-tungstosilicate ion-pair has been reported [11], however, the effective pH of the electrode was 5 and the detection limit was 30-40 mmol dm À3 .…”

Section: Introductionmentioning

confidence: 99%

“…The electrodes are based on incorporation of the chlortetracycline-phosphotungstate [(CTC) 3 PT] or chlortetracycline-phosphomolybdate [(CTC) 3 PM] ion-pair associates in a poly(vinyl chloride) (PVC) membrane plasticized with dibutylphthalate (DBP). The electrodes are used successfully as sensors to determine chlortetracycline hydrochloride in pure solutions and in pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Plastic Membrane Selective Electrodes for Chlortetracyclinium Ion Based on Chlortetracyclinium-Phosphotungstate and Phosphomolybdate Ion-Pair Associates

2001

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Section: Determination Of Ctcclsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Membrane Compositionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Plastic Membrane Selective Electrodes for Chlortetracyclinium Ion Based on Chlortetracyclinium-Phosphotungstate and Phosphomolybdate Ion-Pair Associates

2001

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Determination of tetracyclines in bovine kidney by liquid chromatography/tandem mass spectrometry with on-line extraction and clean-up

Eeckhout

Perez

Claereboudt³

et al. 2000

Rapid Commun. Mass Spectrom.

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A novel, sensitive, high performance liquid chromatography/tandem mass spectrometric (i.e. mass spectrometry/mass spectrometry) method with on-line extraction and clean-up for the screening and confirmation of residues of tetracyclines in kidney has been developed. After liquid extraction of homogenised kidney with McIlvain buffer, an aliquot of the extract is directly injected on the LC/MS/MS system with further extraction and clean-up of the sample on-line. Detection of the analytes was achieved by positive electrospray ionization followed by multiple reaction monitoring. For each tetracycline the collisional decomposition of the protonated molecule to a unique, abundant fragment ion was monitored. The method has been validated for tetracycline, oxytetracycline, chlortetracycline and doxycycline. Calibration curves resulting from spiked blank kidney samples at the 100-1200 microgram/kg level showed good linear correlation. At the level of 600 microgram/kg both within- and between-day precision, as measured by relative standard deviation (RSD), were less than 7%. The limits of detection (LODs) for tetracycline, oxytetracycline, chlortetracycline and doxycyline were 18, 23, 24 and 21 microgram/kg, respectively. The limits of quantification (LOQs) for tetracycline, oxytetracycline, chlortetracycline and doxycyline were 36, 46, 47 and 42 microgram/kg, respectively. The recoveries ranged from 71 to 91%. The procedure provides a rapid, reliable and sensitive method for the determination of residues of tetracyclines in bovine kidney. The advantage of this method over existing methods is its decreased sample preparation and analysis time, which makes the method more suitable for routine analysis.

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Gas and Liquid Chromatography, Column Selection for, in Drug Analysis

Duff

2000

Encyclopedia of Analytical Chemistry

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Within the realm of pharmaceutical drug analyses, two techniques currently dominate the field. High‐performance liquid chromatography (HPLC) is at the forefront, followed somewhat distantly by gas chromatography (GC). HPLC is more prevalent than GC owing mainly to the heat‐labile nature of the majority of drug substances. Still GC continues to play a very active role. In comparison to HPLC and GC, combined, the frequency of use of other techniques is quite small. Perhaps the major reason for this is that chromatography not only involves qualitative and quantitative detection of targeted species, but also provides a medium for isolating these analytes from potential interfering impurities prior to measurement. The proficiency of this combination is unparalleled by any other blending of means to clean up a sample and detect the target compound. These more prominent techniques are often complementary, although each has its own strengths and limitations. General guidelines are given to assist the chromatographer to elect HPLC and/or GC for a particular separation. Considerations for choosing hardware type and sizes, solid support and bonded phase are presented. Particular emphasis is placed on the latest, which is of primary importance for obtaining rugged methods. The reader is directed towards optimum phase selection based on the compound of interest's functional group type, or by its drug activity classification (e.g. barbiturate, cardiovascular, etc.). Assistance in developing new methods frequently arises when starting with an existing published method for a compound with similar structure. This article provides 514 references for more in‐depth separation information. In addition, background knowledge on bonded‐phase chemistry, chromatographic modes of separation, approaches to chromatographic chiral drug discrimination and processes for developing rugged methods is contributed.

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Rapid determination of tetracycline antibiotics in serum by reversed-phase high-performance liquid chromatography with fluorescence detection

Cited by 27 publications

References 10 publications

Plastic Membrane Selective Electrodes for Chlortetracyclinium Ion Based on Chlortetracyclinium-Phosphotungstate and Phosphomolybdate Ion-Pair Associates

Plastic Membrane Selective Electrodes for Chlortetracyclinium Ion Based on Chlortetracyclinium-Phosphotungstate and Phosphomolybdate Ion-Pair Associates

Determination of tetracyclines in bovine kidney by liquid chromatography/tandem mass spectrometry with on-line extraction and clean-up

Gas and Liquid Chromatography, Column Selection for, in Drug Analysis

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