Rapid determination of tacrolimus and sirolimus in whole human blood by direct coupling of solid-phase microextraction to mass spectrometry via microfluidic open interface

Nazdrajić, Emir; Tascón, Marcos; Rickert, Daniel A.; Gómez‐Ríos, Germán Augusto; Kulasingam, Vathany; Pawliszyn, Janusz

doi:10.1016/j.aca.2020.11.056

Cited by 37 publications

(18 citation statements)

References 47 publications

(76 reference statements)

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“…For applications where SPME is directly coupled to mass spectrometry (SPME-MS) with the aim of achieving rapid analysis, the mass transfer resistance in the extraction phase is a significant issue, as the desorption times used are in the order of seconds (microfluidic open interface and coated blade spray) or lower (probe electrospray ionization). − When using a thick extraction phase, it is simply not enough to obtain the best possible desorption efficiency. Therefore, the extraction phase must be modified in response to the increase in surface area; specifically, this modification largely entails carefully optimizing the percentage of sorbent in the extraction phase and reducing the extraction phase thickness.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Sorbent Particles in a Binder on the Mass Transfer Kinetics in Separation Media: In Silico Study and Experimental Verification

2021

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Selecting the optimal binder and the sorbent affinity for selected compounds can cause the composite to behave either as an efficient extraction coating, as a permeable membrane, or as an impermeable barrier. If the compound partitions onto the sorbent with high preference, it becomes stationary and the composite behaves as an impermeable barrier, while appropriately optimized affinity will result in effective permeation. To understand this phenomenon, we utilize solid-phase microextraction to characterize the mass transfer attributes of different separation composites. Our results indicate that for strong sorbents, the extraction rate is primarily controlled by the diffusion in the extraction phase rather than the sample matrix, even if it is relatively thin. Low analyte diffusion is caused by the retarding force generated by the partitioning of analytes into the sorbent, as migration through the composite is driven by the unbound form of the compound in the binder. One of the main contributions of this work is that an understanding of the extraction composite parameters that control mass transfer during extraction enables better optimization of binder/sorbent extraction phase composition for a given application. Another contribution of this work shows how a heterogeneous coating model can be simplified into a homogeneous coating model. The developed models enable an enhanced understanding of mass transfer kinetics, and they provide insight into how to optimize the extraction phase parameters for a given method involving sorbent particles in polymeric media, including membranes and paints, in addition to extraction coatings.

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Section: Resultsmentioning

confidence: 99%

The Effect of Sorbent Particles in a Binder on the Mass Transfer Kinetics in Separation Media: In Silico Study and Experimental Verification

2021

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“…With regard to instrumental analysis, there are many options for the direct coupling of the SPME probe with analytical instrumentation, particularly mass spectrometers [ 61 ]. A strategy which seems to be specifically suitable for this purpose is the microfluidic open interface, presented lately for the fast monitoring of drugs [ 62 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Phenotyping of Gliomas: What Can We Get with Simplified Protocol for Intact Tissue Analysis?

Goryńska

Chmara

Kupcewicz

et al. 2022

Cancers

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Glioblastoma multiforme is one of the most malignant neoplasms among humans in their third and fourth decades of life, which is evidenced by short patient survival times and rapid tumor-cell proliferation after radiation and chemotherapy. At present, the diagnosis of gliomas and decisions related to therapeutic strategies are based on genetic testing and histological analysis of the tumor, with molecular biomarkers still being sought to complement the diagnostic panel. This work aims to enable the metabolomic characterization of cancer tissue and the discovery of potential biomarkers via high-resolution mass spectrometry coupled to liquid chromatography and a solvent-free sampling protocol that uses a microprobe to extract metabolites directly from intact tumors. The metabolomic analyses were performed independently from genetic and histological testing and at a later time. Despite the small cohort analyzed in this study, the results indicated that the proposed method is able to identify metabolites associated with different malignancy grades of glioma, as well as IDH and 1p19q codeletion mutations. A comparison of the constellation of identified metabolites and the results of standard tests indicated the validity of using the characterization of one comprehensive tumor phenotype as a reflection of all diagnostically meaningful information. Due to its simplicity, the proposed analytical approach was verified as being compatible with a surgical environment and applicable for large-scale studies.

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“…The commercial fibers can be directly bought with good repeatability and satisfactory enrichment capacity, which are usually selected for the extraction of VOCs and metabolites with different physicochemical properties. , Hydrophilic–lipophilic balance (HLB) particles have been explored by the group of Prof. Janusz Pawliszyn as novel SPME coating materials, which can be mixed with polyacrylonitrile dimethylformamid to produce bio-SPME fibers. These prepared bio-SPME fibers have been used for the therapeutic drug monitoring of tranexamic acid (TXA) from plasma and urine of patients with chronic renal dysfunction, as well as the rapid determinations of four relevant immunosuppressive drugs (tacrolimus, sirolimus, everolimus, and cyclosporine A) in whole human blood . The emerging functional materials provide a good chance for the steadily enhanced extraction of target analytes in human urine and serum, including perylene diimide-POSS network, chitosan nanocomposite incorporated with polyoxomolibdate nanocluster/graphene oxide, HKUST-1, polypyrrole, magnetic Fe 3 O 4 @MIL-101(Cr) nanoparticles/polyaniline nanocomposite, and molecularly imprinted polymers. − Moreover, the extraction efficiency of self-made SPME fiber can be further enhanced by designing the adsorbents based on the structure property of the target analytes.…”

Section: Spme In Biological Analysismentioning

confidence: 99%

Latest Improvements and Expanding Applications of Solid-Phase Microextraction

et al. 2023

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Rapid determination of tacrolimus and sirolimus in whole human blood by direct coupling of solid-phase microextraction to mass spectrometry via microfluidic open interface

Cited by 37 publications

References 47 publications

The Effect of Sorbent Particles in a Binder on the Mass Transfer Kinetics in Separation Media: In Silico Study and Experimental Verification

The Effect of Sorbent Particles in a Binder on the Mass Transfer Kinetics in Separation Media: In Silico Study and Experimental Verification

Metabolomic Phenotyping of Gliomas: What Can We Get with Simplified Protocol for Intact Tissue Analysis?

Latest Improvements and Expanding Applications of Solid-Phase Microextraction

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