Rapid Determination of Seven Fungicides in Citrus Fruits

Abstract

A rapid and simple determination method of seven fungicides, thiabendazole TBZ , pyrimethanil PYR , o-phenylphenol OPP , fludioxonil FLD , azoxystrobin AZX , imazalil IMZ and diphenyl DP in citrus fruits by LC-MS and HPLC-FL was developed. The seven fungicides were extracted with acetonitrile from citrus fruits and cleaned up with Z-Sep/C18 cartridges. The LC separation was performed on a phenyl-hexyl column with methanol-acetonitrile-10 mmol/L ammonium formate 10 : 35 : 55 as a mobile phase. The recoveries from citrus fruits fortified with the compounds at the MRLs and at 0.1 μ g/g ranged from 85.4 to 106.3 and from 75.8 to 99.7 , respectively. The quantitation limits S/N 10 were 0.03-0.07 μ g/g.

Simultaneous Determination of Seven Kinds of Fungicides in Citrus Fruits by Gas Chromatograghy/Mass Spectrometry

“…Yoshioka et al 3) reported that the sensitivities for DP and OPP were low on LC-MS (ESI) analysis, so they determined these two fungicides by HPLC equipped with a fluorescence detector, while AZO, FLUDI, IMZ, PYRI and TBZ were analysed by LC-MS. However, the sensitivities for AZO and FLUDI also seemed to be insufficient for application in real matrices.…”

“…1. Although Yoshioka et al 3) recently reported determination methods for AZO, FLUDI, IMZ, PYRI and TBZ by LC-MS and for DP and OPP by HPLC, a GC-MS method could monitor all seven fungicides in a single run. Therefore, we developed a GC-MS method for simultaneous identification and quantification of these fungicides and confirmed its suitability for the determination of the fungicides in citrus fruits.…”