Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer

Heaps, Amy; Sobolewski, Michele D.; Jacobs, Jana L.; Gordon, Kelley C.; Haidar, Ghady; Mellors, John W.; Parikh, Urvi M.

doi:10.1016/j.jviromet.2023.114726

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Pseudovirus Neutralizing Antibody Titers1

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2023

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“…Half-maximal neutralizing antibody titers (NT50) were generated using a lentiviral pseudovirus assay, as previously described 47 . Briefly, PSV was generated in HEK293T cells co-transfected with SARS-CoV-2 spike expression and pNL4-3.luc.R-E-mCherry-luciferase reporter packaging plasmids.…”

Section: Pseudovirus Neutralizing Antibody Titersmentioning

confidence: 99%

Early antiviral CD4 and CD8 T cell responses and antibodies are associated with upper respiratory tract clearance of SARS-CoV-2

Ramirez,

Lopez,

Faraji

et al. 2023

Preprint

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T cells are involved in protective immunity against numerous viral infections. Limited data have been available regarding roles of human T cell responses controlling SARS-CoV-2 viral clearance in primary COVID-19. Here, we examined longitudinal SARS-CoV-2 upper respiratory tract viral RNA levels and early adaptive immune responses from 95 unvaccinated individuals with acute COVID-19. Acute SARS-CoV-2-specific CD4 and CD8 T cell responses were evaluated in addition to antibody responses. Most individuals with acute COVID-19 developed rapid SARS-CoV-2-specific T cell responses during infection, and both early CD4 T cell and CD8 T cell responses correlated with reduced upper respiratory tract SARS-CoV-2 viral RNA, independent of neutralizing antibody titers. Overall, our findings indicate a distinct protective role for SARS-CoV-2-specific T cells during acute COVID-19.

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Section: Pseudovirus Neutralizing Antibody Titersmentioning

confidence: 99%