Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition

Abstract

Using a highly sensitive single photon counter, a spontaneous chemiluminescence (CL) study on rice (Oryza sativa L.) seeds stored in different years was carried out. We first observed that the degree of ageing in rice seeds was related to the intensity of spontaneous CL during early imbibition (0-30 min). Rice seeds stored for a shorter time had a stronger intensity of CL in early imbibition. The germination rate of rice seeds showed an obvious positive correlation with the intensity of spontaneous CL. Singlet oxygen ((1)O(2)) in rice seeds during early imbibition was investigated by a CL method using a cypridina luciferin analogue, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo [1,2alpha] pyrazin-3-one (MCLA), as a selective CL probe. Additional experimental evidence for the formation of (1)O(2) came from the quenching effect of sodium azide (NaN(3)) on MCLA-mediated rice seeds' CL. Analysis based on the experimental results demonstrated that spontaneous CL in rice seeds during early imbibition was mainly contributed by singlet oxygen ((1)O(2)).

Spontaneous ultra-weak light emissions from wheat seedlings are rhythmic and synchronized with the time profile of the local gravimetric tide

“…As a result, UWPE from seeds has been employed as a rapid means of evaluating seed quality and viability. For cereal seeds, for example, it is possible, after only a few minutes of imbibition, to distinguish those seeds which will produce stronger sprouts upon germination from those which will be weaker (Chen et al 2003). Then, during the subsequent growth period, the whole of each seedling emits light, the source of the luminescence being the radicle, the cotyledon, and the endosperm, the latter tissue, perhaps because of its greater mass and rapid catabolic metabolism, producing the greatest signal (Chao 1998).…”

Chemiluminescence during rice seed imbibition at different temperatures

“…Q 10 = 2) (3) in the range 303-323°K are critical for the generation of excited electronic states which lead to the observed CL. Our previous results (6) showed that rice seeds with a longer storage time had a lower intensity of CL during early imbibition, and the germination rate of rice seeds showed a significant positive correlation with the intensity of spontaneous CL. Our previous report suggested that there was production of active oxygen (6).…”

“…Our previous results (6) showed that rice seeds with a longer storage time had a lower intensity of CL during early imbibition, and the germination rate of rice seeds showed a significant positive correlation with the intensity of spontaneous CL. Our previous report suggested that there was production of active oxygen (6). We believe that the production of active oxygen must be related to enzyme reactions.…”

“…They suggest that physical and not enzymatic processes (Q 10 = 2) are critical for the generation of excited electronic states, which might lead to the observed CL (3). Our previous report (6) showed that rice seeds with a longer storage time have a lower intensity of CL during early imbibition. The germination rate of rice seeds correlates well with the intensity of CL.…”

Determination of spectrum and different temperature of spontaneous chemiluminescence in rice seeds during early imbibition

“…It has been shown that the interaction between water and dry cereal grains, flour and bread products results in much stronger CL [3,4,5]. Our previous report [6] showed that rice seeds with longer storage time had lower intensity of spontaneous CL during early imbibition, the germination rate of rice seeds showed a significant positive correlation with the intensity of spontaneous CL, the experimental results demonstrated that UCL in rice seeds during early imbibition could be attributed mainly to singlet oxygen ( 1 O 2 ). Seed germination can be separated into three distinct phases [7].…”