Rapid determination of polycyclic aromatic hydrocarbons (PAHs) in zebrafish eleutheroembryos as a model for the evaluation of PAH bioconcentration

El-Amrani, S.; Sanz‐Landaluze, Jon; Guinea, J.; Cámara, Carmen

doi:10.1016/j.talanta.2012.11.025

Cited by 20 publications

(12 citation statements)

References 39 publications

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“…Although each of the studied substances bioconcentrated in embryos, biotransformation and elimination capacities varied among chemicals. Similarly, uptake studies in slightly older eleutheroembryo and larval stages are not much more abundant and are largely focused on the same or similar chemical classes, including pesticides (organophosphates, organochlorines, herbicides [43] and pyrethroids [44]), PAHs [45], and the antibacterial agent triclosan [46]. Despite occasional compounds failing to reach steady state [43], a number of these eleutheroembryo and larval studies estimated BCF values similar to those obtained with adult fish.…”

Section: Resultsmentioning

confidence: 99%

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Kristofco

Haddad

Chambliss³

et al. 2018

Enviro Toxic and Chemistry

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The zebrafish fish embryo toxicity (FET) test is increasingly employed for alternative toxicity studies, yet our previous research identified increased sensitivity of zebrafish slightly older than embryos employed in FET methods (0-4 d postfertilization [dpf]). We identified rapid steady-state accumulation of diphenhydramine across zebrafish embryo and larval stages. However, significantly (p < 0.05) lower accumulation was observed at 48 h compared to 96 h in chorionated and dechorionated embryos (0-4 dpf), but not in zebrafish at 7 to 11 and 14 to 18 dpf. Increased uptake and toxicity of diphenhydramine was further observed in zebrafish at 7 to 11 and 14 to 18 dpf compared with 0-4 dpf embryos with chorion or dechorionated, which indicates that differential zebrafish sensitivity with age is associated with accumulation resulting from gill and other toxicokinetic and toxicodynamic changes during development. Environ Toxicol Chem 2018;37:1175-1181. © 2017 SETAC.

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Section: Resultsmentioning

confidence: 99%

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Kristofco

Haddad

Chambliss³

et al. 2018

Enviro Toxic and Chemistry

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“…7 Internal concentrations of organic compounds internalized by ZFE were successfully correlated to toxicological effects. [8][9][10] However, for metal toxicity, not only the internalized but also the attached fraction may affect organisms. But little knowledge on the uptake of metals by ZFE and the underlying kinetic processes exists.…”

Section: Introductionmentioning

confidence: 99%

Metal uptake and distribution in the zebrafish (Danio rerio) embryo: differences between nanoparticles and metal ions

Böhme

Baccaro

Schmidt

et al. 2017

Environ. Sci.: Nano

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“…For the analysis of exposure media, volumes of 500 μl were mixed immediately with 1 ml of acetonitrile, shaken vigorously, filtered through 0.22 μm PTFE syringe filters and finally measured with the HPLC‐fluorescence detector, using the conditions summarized in the section “Polycyclic aromatic hydrocarbon analysis.” Samples were previously spiked with 40 μl of a 500 ng ml −1 solution of fluorene or anthracene as internal standard. The analytical methodology used for PAHs determination in zebrafish embryos was adapted from a previously developed methodology (El‐Amrani et al ., ). Embryos, in groups of 20, with an overall wet weight of approximately 13.3 mg, were treated with 500 μl of acetonitrile and sonicated during 40 s (using the ultrasound probe at 130 W and 20 kHz with an amplitude of 40%).…”

Section: Methodsmentioning

confidence: 97%

Detection of exposure effects of mixtures of heavy polycyclic aromatic hydrocarbons in zebrafish embryos

et al. 2016

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In this study we evaluated the exposure effects of mixtures of five polycyclic aromatic hydrocarbons (PAHs); namely, benzo[a]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene and chrysene on zebrafish embryos. Supplementation of the exposure media with 0.45% dimethyl sulfoxide and 50 ppm of Tween 20 could guarantee the solubilization and stabilization of the PAHs up to 24 h without affecting the embryos development. The exposure effects were tested by detecting the differential expression of a number of genes related to the aryl hydrocarbon receptor gene battery. Effects were detectable already after 6 h of exposure. After 24 h of exposure, all PAHs, except for benzo[a]anthracene, acted as potent inducers of the gene cyp1a1. Benzo[k]fluoranthene was the major inducer; the effect caused by the mixture at the lower concentration tested (1 ng ml ) was dominated by its presence. However, in the mixture at the highest concentration tested (10 ng ml ) it caused less induction and was not dominant. No significant bioaccumulation values were detected on embryos exposed to the PAHs tested in this study; however, the results obtained, indicated that PAHs undergo a very rapid metabolization inside the embryos, and that those biotransformation products yield changes on the expression of genes involved in the aryl hydrocarbon receptor pathway. Future work should focus on identification of the PAH metabolization products and on the effect of these metabolites on toxicity. Copyright © 2016 John Wiley & Sons, Ltd.

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Rapid determination of polycyclic aromatic hydrocarbons (PAHs) in zebrafish eleutheroembryos as a model for the evaluation of PAH bioconcentration

Cited by 20 publications

References 39 publications

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Metal uptake and distribution in the zebrafish (Danio rerio) embryo: differences between nanoparticles and metal ions

Detection of exposure effects of mixtures of heavy polycyclic aromatic hydrocarbons in zebrafish embryos

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