Rapid determination of nucleosides, nucleobases and free amino acids in brown seaweeds using ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

Yan, Chao‐Guo; Duan, Jin‐Ao; Guo, Jianming; Guo, Sheng; Zhao, Jinlong

doi:10.1007/s10811-013-0079-3

Cited by 17 publications

(11 citation statements)

References 33 publications

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“…However, there is a wide variation in the composition of protein in foods [40] and in particular algae [44,45]. Biomass may also contain a proportion of nitrogen that is not associated with proteins, but with compounds such as DNA, pigments, and free amino acids [44,46], and thus the commonly used multiplier of 6.25 causes the protein content to be overestimated [44,47]. Factors for common foods vary between 5.2 and 6.3 [40] and for algae 3.75-6.4 [44,48].…”

Section: Proteinsmentioning

confidence: 99%

Golden Tides: Problem or Golden Opportunity? The Valorisation of Sargassum from Beach Inundations

Milledge

Harvey

2016

JMSE

163

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Abstract:In recent years there have been massive inundations of pelagic Sargassum, known as golden tides, on the beaches of the Caribbean, Gulf of Mexico, and West Africa, causing considerable damage to the local economy and environment. Commercial exploration of this biomass for food, fuel, and pharmaceutical products could fund clean-up and offset the economic impact of these golden tides. This paper reviews the potential uses and obstacles for exploitation of pelagic Sargassum. Although Sargassum has considerable potential as a source of biochemicals, feed, food, fertiliser, and fuel, variable and undefined composition together with the possible presence of marine pollutants may make golden tides unsuitable for food, nutraceuticals, and pharmaceuticals and limit their use in feed and fertilisers. Discontinuous and unreliable supply of Sargassum also presents considerable challenges. Low-cost methods of preservation such as solar drying and ensiling may address the problem of discontinuity. The use of processes that can handle a variety of biological and waste feedstocks in addition to Sargassum is a solution to unreliable supply, and anaerobic digestion for the production of biogas is one such process. More research is needed to characterise golden tides and identify and develop commercial products and processes.

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Section: Proteinsmentioning

confidence: 99%

Golden Tides: Problem or Golden Opportunity? The Valorisation of Sargassum from Beach Inundations

Milledge

Harvey

2016

JMSE

163

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“…As these amino acids and many more have also been reported to exist as free amino acids in brown, red and green seaweeds [74][75][76], the higher amino acid content in relation to the protein content (Table 11) is likely due to free amino acids and non-protein amino acids from the hydrolysis of peptides. The non-protein nitrogen content in L. japonica for example has been reported as 60% of the total nitrogen content [8].…”

Section: Amino Acid Profilingmentioning

confidence: 99%

The nutritional aspects of biorefined Saccharina latissima, Ascophyllum nodosum and Palmaria palmata

Schiener

SuFen

Theodoridou

et al. 2016

Biomass Conv. Bioref.

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The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that: • a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders. Please consult the full DRO policy for further details. Abstract The chemical profile of biorefined Saccharina latissima, Ascophylum nodosum and Palmaria palmata after carbohydrate and polyphenol extraction was analysed with the aim to evaluate the nutritional aspects of biorefined seaweeds as a novel animal feed supplement. Optimised enzymatic saccharification has been used to show that the protein concentration in the residue of Palmaria palmata and Ascophylum nodosum can be increased by more than two fold. Nutritional value of the residue was further enhanced through an increase in total amino acids and fatty acids. As a consequence of removal of inorganic elements such as sodium, potassium and chloride, the total solid and ash content of all three seaweeds was reduced by around 40%. In contrast, divalent metals such as iron and zinc, as well as silicon accumulated in all three residues. Potentially harmful components such as arsenic and iodine were reduced only in brown biorefined seaweeds, whilst in biorefined P. palmata iodine increased by 39% compared to a 24% decline of arsenic. Polyphenol removal in all three seaweeds was >80% and, in combination with enzymatic saccharification, enhanced protein recovery in A. nodosum. This highlights the potential of biorefinery concepts to generate multiple products from seaweed such as extracts enriched in polyphenols and carbohydrates and residue with higher protein and lipid content.

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“…In previous reports, the analysis of FAAs and Ns have been used as indicators to evaluate the quality of some Chinese medicines and foods, such as: Abelmoschus manihot ; Ziziphus plants; Ganoderma lucidum ; Sipunculus nudus ; clam; brown seaweeds; bamboo shoots; and meat (Cao, Duan, Guo, Guo, & Zhao, ; Du et al, ; Elaloui et al, ; Ge et al, ; Szterk, ; Szterk & Roszko, ; Wang et al, ; Xu et al, ; Yang, Zhang, & Li‐Ya, ). However, current methods that test for FAAs and Ns were lacking or non‐existent.…”

Section: Introductionmentioning

confidence: 99%

“…Abbreviations used: FFA, free amino acid; LOD, limits of detection; LOQ, limits of quantification; Ns, nucleobases; PCA, principal components analysis; RSD, relative standard deviation; SSP, Semen sojae praeparatum; UFLC, ultra-fast liquid chromatography and meat (Cao, Duan, Guo, Guo, & Zhao, 2014;Du et al, 2015;Elaloui et al, 2014;Ge et al, 2016;Szterk, 2015;Szterk & Roszko, 2014;Wang et al, 2016;Xu et al, 2016;Yang, Zhang, & Li-Ya, 2011).…”

Section: Introductionmentioning

confidence: 99%

Contents variation analysis of free amino acids, nucleosides and nucleobases in semen sojae praeparatum fermentation using UFLC‐QTRAP MS

Chai

Cui

Shan

et al. 2017

Biomedical Chromatography

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UFLC-QTRAP MS was used to develop a sensitive and rapid method of evaluating content variation during Semen sojae praeparatum (SSP) fermentation. It did this through the simultaneous quantification of 22 free amino acids and 16 nucleosides and nucleobases in the raw materials and processed products of SSP. The method was shown to be reproducible and accurate. The limits of detection and quantity values were 0.09-168.75 and 0.31-562.50 ng/mL for the 38 analytes, respectively. The data were examined through principal components analysis to compare the content variations. The quantitative results showed that the ingredients were properly determined in most of the samples and were converted regularly throughout the SSP fermentation process. These results correspond to the morphologic changes and principal components analysis results.

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Rapid determination of nucleosides, nucleobases and free amino acids in brown seaweeds using ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

Cited by 17 publications

References 33 publications

Golden Tides: Problem or Golden Opportunity? The Valorisation of Sargassum from Beach Inundations

Golden Tides: Problem or Golden Opportunity? The Valorisation of Sargassum from Beach Inundations

The nutritional aspects of biorefined Saccharina latissima, Ascophyllum nodosum and Palmaria palmata

Contents variation analysis of free amino acids, nucleosides and nucleobases in semen sojae praeparatum fermentation using UFLC‐QTRAP MS

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