Rapid determination of drugs in biofluids by capillary electrophoresis Measurement of antipyrine in saliva for pharmacokinetic studies

Perrett, David; Ross, Gordon

doi:10.1016/0021-9673(95)00101-r

Cited by 49 publications

(14 citation statements)

References 12 publications

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“…Interestingly, a calibration curve prepared from aqueous standards gave a significant bias compared to quantitation using standard addition, the latter procedure giving results which accorded well with results from the Greiss reaction or NO chemiluminescence. This matrix effect may be due to the viscosity of the sample affecting hydrodynamic injection, as previously noted in analysis of saliva [102]. Ascorbic and uric acid have been determined in aqueous humor by CZE after 1:20 dilution of the sample with water [155].…”

Section: Aqueous and Vitreous Humormentioning

confidence: 99%

“…Thormann et al used saliva as one of several matrices in investigations of suitable MEKC conditions for the direct injections of biofluids [100,101]. Antipyrene was measured in saliva using MEKC with SDS as micellar additive which minimized the effects of the sample matrix and helped ensure repeatability of the assay [102]. Enantiomers of albendazole sulfoxide were concentrated from saliva by liquid-liquid extraction (LLE) before separation using CE with sulfated-␤-cyclodextrin (CD) as an additive [103].…”

Section: Salivamentioning

confidence: 99%

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Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

Lloyd

2008

Journal of Chromatography B

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Section: Aqueous and Vitreous Humormentioning

confidence: 99%

Section: Salivamentioning

confidence: 99%

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

Lloyd

2008

Journal of Chromatography B

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“…However, also in this case elevated run times are necessary to separate the three phosphate forms due to the high differences in their charge density. In order to reduce the analysis time in CE, short total capillary lengths [16][17][18][19] and/or high voltages [17] are commonly recommended. However, in these approaches high currents may be generated with a loss of efficiency and reproducibility [19].…”

Section: Intracellular Adenosinementioning

confidence: 99%

“…In order to reduce the analysis time in CE, short total capillary lengths [16][17][18][19] and/or high voltages [17] are commonly recommended. However, in these approaches high currents may be generated with a loss of efficiency and reproducibility [19]. The analysis time can also be reduced using a short-end injection procedure where the separation length is decreased by injecting the sample at the capillary end closest to the detector window (outlet).…”

Section: Intracellular Adenosinementioning

confidence: 99%

Intracellular adenosine 5′‐triphosphate, adenosine 5′‐diphosphate, and adenosine 5′‐monophosphate detection by short‐end injection capillary electrophoresis using methylcellulose as the effective electroosmostic flow suppressor

et al. 2008

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We present a new rapid CE method to measure adenine nucleotides adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), and adenosine 5'-monophosphate (AMP) in cells. The short-end injection mode allows a decrease in the analysis time by injecting samples at the outlet end of a silica capillary closest to the detection window, reducing the migration distance. Moreover, the use of methylcellulose (MC) as run buffer additive to suppress EOF permits to further reduce the migration times of analytes. Thus, when a capillary with an effective length of 10.2 cm was used with a 60 mmol/L sodium acetate buffer pH 3.80 in the presence of 0.01% of MC, the migration time of analytes were 1.35 min for ATP, 1.85 min for ADP, and 4.64 min for AMP. These conditions gave a good reproducibility for intra- and interassay (CV <4 and 8%, respectively) and all the procedure demonstrated an excellent analytical recovery (from 98.3 to 99 %). The method suitability was proved both on red blood cells and in spermatozoa. We compared our proposed method to a spectrophotometric assay, by measuring ATP levels in 40 spermatozoa samples. The obtained data were analyzed by the Passing and Bablok regression and Bland-Altman test.

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“…1 It is an official drug in USP 2 and BP 3 pharmacopeia. Several analytical methods were reported for the determination of AN either alone or coformulated with other drugs including spectrophotometric, 4,5 chemometric, 6,7 HPLC, 8,9 TLC, 10,11 GC,12,13 , capillary zone electrophoresis 14 and non aqueous titration 15 methods. Benzocaine (BE, Fig.…”

Section: Introductionmentioning

confidence: 99%

Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

Merey

2016

Bulletin of Faculty of Pharmacy, Cairo University

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Antipyrine and benzocaine are formulated together for the treatment of ear inflammation and to relieve pain. Four spectrophotometric methods were developed for the simultaneous determination of antipyrine (AN) and benzocaine (BE) in their combined dosage form. Method A depends on applying dual wavelength method where antipyrine was determined by measuring the absorbance at 254.1 and 309.1 nm (corresponding to zero difference of benzocaine), while the absorbance difference at 230.1 and 263.5 nm (corresponding to zero difference of antipyrine) was selected for benzocaine determination in the laboratory prepared spectrum. Method B depends on measuring the peak amplitude of first derivative at 305 nm for calculating benzocaine concentration then the total concentration of both drugs was determined using isoabsorptive point at 257.4 nm (antipyrine concentration was then calculated by subtraction). Method C is based on measuring the peak difference of the ratio spectra at Dp (239.1-285 nm) and Dp (301.4-250 nm) for the determination of antipyrine and benzocaine, respectively. Method D depends on measuring peak to peak amplitude of the first derivative of ratio spectra at (234.5 + 244.2 nm) and peak amplitude at 295.5 nm for the determination of antipyrine and benzocaine, respectively. The proposed methods were validated and applied for the analysis of antipyrine and benzocaine in their laboratory prepared mixtures and pharmaceutical formulation. Statistical comparison between the results of the proposed methods and those of the reported methods showed no significant difference. Ó 2016 Production and hosting by Elsevier B.V. on behalf of Faculty of Pharmacy, Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).

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Rapid determination of drugs in biofluids by capillary electrophoresis Measurement of antipyrine in saliva for pharmacokinetic studies

Cited by 49 publications

References 12 publications

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

Intracellular adenosine 5′‐triphosphate, adenosine 5′‐diphosphate, and adenosine 5′‐monophosphate detection by short‐end injection capillary electrophoresis using methylcellulose as the effective electroosmostic flow suppressor

Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

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