Rapid Determination of Beryllium by a Direct-Reading Atomic Absorption Spectrophotometer

Bokowski, D. L.

doi:10.1080/00028896809343038

Cited by 16 publications

(5 citation statements)

References 7 publications

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“…Samples for atomic absorption spectrophotometry (AAS) were prepared following wet digestion method using solution of nitric acid and perchloric acid in the ratio of 4 : 1 and brought to a constant volume of each. Determination of beryllium content was performed with AAS (VP 90; Thermo Electron Corporation, Cambridge, UK) at a wavelength of 235 nm [26].…”

Section: Methodsmentioning

confidence: 99%

Pharmacological intervention of tiferron and propolis to alleviate beryllium‐induced hepatorenal toxicity

Nirala

Bhadauria

Shukla

et al. 2008

Fundamemntal Clinical Pharma

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Intervention of chelating agent tiferron (sodium-4,5-dihydroxy-1,3-benzene disulfonate; 300 mg/kg, intraperitoneal) with propolis (honey beehive product; 200 mg/kg, p.o.) was evaluated to encounter the characteristic biochemical and ultra-morphological alterations following subchronic exposure to beryllium. Female albino rats were challenged with beryllium nitrate (1 mg/kg, i.p.) daily for 28 days followed by treatment of the above-mentioned therapeutic agents either individually or in combination for five consecutive days. Exposure to beryllium increased its concentration in the serum, liver and kidney, and caused significant alterations in cytochrome P450 activity, microsomal lipid peroxidation and proteins. Activities of alkaline phosphatase, lactate dehydrogenase, gamma-glutamyl transpeptidase, bilirubin, creatinine and urea in the serum and activity of acid phosphatase, alkaline phosphatase, adenosine triphosphatase, glucose-6-phophatase and succinic dehydrogenase in the liver and kidney were significantly altered after beryllium administration. Beryllium exposure also induced severe hepatorenal alterations at histopathological and ultra-morphological level. Tiferron along with propolis dramatically reversed the alterations in all the variables more towards control rather than their individual treatment. The study concludes that pharmacological intervention of tiferron and propolis is beneficial in attenuating beryllium-induced systemic toxicity.

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Section: Methodsmentioning

confidence: 99%

Pharmacological intervention of tiferron and propolis to alleviate beryllium‐induced hepatorenal toxicity

Nirala

Bhadauria

Shukla

et al. 2008

Fundamemntal Clinical Pharma

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“…Samples for atomic absorption spectrophotometry (AAS) were prepared following wet digestion method using solution of nitric acid and perchloric acid in the ratio of 4:1 and maintained to a constant volume. Beryllium content was determined with AAS (VP 90, Thermo Electron Corporation, U.K.) at wavelength 235 nm according to the method of Bokowski [38].…”

Section: Beryllium Mobilizationmentioning

confidence: 99%

Amelioration of beryllium induced alterations in hepatorenal biochemistry and ultramorphology by co-administration of tiferron and adjuvants

et al. 2007

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Influence of adjuvants i.e., alpha-tocopherol (25 mg/kg, p.o.) and piperine (10 mg/kg, p.o.) on therapeutic potential of chelator tiferron (300 mg/kg, i.p.) was evaluated to encounter toxicogenic events of beryllium exposure. Albino rats were exposed to beryllium nitrate (1 mg/kg, i.p.) daily for 28 days followed by treatment of aforesaid therapeutic agents for 5 consecutive days. Results were considered to be significant at p < or =0.01 and p < or =0.05. Exposure to beryllium increased its concentration in liver, kidney and serum causing significant alterations in the activity of CYP-450 2E1 system, microsomal lipid peroxidation and protein; alkaline phosphtase, lactate dehydrogenase, gamma-glutamyl transpeptidase, bilirubin, creatinine and urea in serum; activity of acid phosphatase, alkaline phosphatase, adenosine triphosphatase, glucose-6-phosphatase and succinic dehydrogenase in liver and kidney. Beryllium exposure also induced severe alterations in histopathology and ultramorphology of liver and kidney proving its toxic consequences at cellular level. Tiferron along with adjuvants dramatically reversed alterations of all variables more towards control rather than individual treatment. Study concluded that tiferron in combination with alpha-tocopherol and piperine respectively was beneficial in diluting beryllium induced systemic toxicity; however, combination of tiferron and piperine presented more pronounced therapeutic potential.

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“…Digested samples were diluted to 10 ml with distilled water and beryllium was extracted by cupferron solution and MIBK (methylisobutyl ketone) and the MIBK layer was read by AAS (Bokowski 1968). …”

Section: Analysis Of Berylliummentioning

confidence: 99%

Protective effect of Tiron (4,5-dihydroxybenzene-1,3-disulfonic acid disodium salt) against beryllium-induced maternal and fetal toxicity in rats

Sharma

Shah

Shukla

2002

Archives of Toxicology

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The efficacy of Tiron (4,5-dihydroxybenzene 1,3-disulfonic acid disodium salt) was examined in the treatment of beryllium-induced maternal and developmental toxicity in rats. Single administration of beryllium nitrate at a dose of 50 mg/kg (i.m.) on day 13 of gestation caused reductions in fetal and placental weights, the number of implantation sites and number of corpora lutea, as well as causing post-implantation loss, stunted growth, increase in the number of resorptions, and also a disturbed sex ratio. Maternal toxicity was demonstrated by reduction in body weight gain. Administration of beryllium also showed significant alteration in the hematological and biochemical indices of the mother as well as the fetus. Marked decreases were recorded in hemoglobin percentage, blood sugar levels, serum protein contents and serum alkaline phosphatase activity. By contrast, significant elevation was found in the activity of transaminases (aspartate aminotransferase and alanine aminotransferase). Tissue protein contents, glycogen contents, activities of alkaline phosphatase, adenosine triphosphatase and succinic dehydrogenase of kidney, lungs and uterus, and maternal and fetal liver all showed significantly decreased values after beryllium exposure, and remarkable elevation was observed in acid phosphatase, glucose-6-phosphatase and hepatic lipid peroxidation. These parameters were restored considerably with administration of 471 mg/kg i.m. Tiron from days 14 to 18 of gestation. Atomic absorption spectrophotometry also revealed a high concentration of beryllium in different organs of pregnant rats. Interestingly, a small amount of metal ion was also detected in the fetus and reduced accumulation of beryllium was noticed after Tiron treatment.

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Rapid Determination of Beryllium by a Direct-Reading Atomic Absorption Spectrophotometer

Cited by 16 publications

References 7 publications

Pharmacological intervention of tiferron and propolis to alleviate beryllium‐induced hepatorenal toxicity

Pharmacological intervention of tiferron and propolis to alleviate beryllium‐induced hepatorenal toxicity

Amelioration of beryllium induced alterations in hepatorenal biochemistry and ultramorphology by co-administration of tiferron and adjuvants

Protective effect of Tiron (4,5-dihydroxybenzene-1,3-disulfonic acid disodium salt) against beryllium-induced maternal and fetal toxicity in rats

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