Rapid determination of 20-hydroxyecdysteroids in complex mixtures by solid-phase extraction and mass spectrometry

Píš, Jaroslav; Hykl, Jiří; Vaisar, Tomáš; Harmatha, Juraj

doi:10.1016/0021-9673(94)85208-1

Cited by 9 publications

(1 citation statement)

References 9 publications

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“…The main chemical transitions of ecdysteroids are: hydroxylation (usually at position 5,11,25,26), oxidation (position 26), βand γ-lactam ring formation (mainly side chain), alkylation (branched side chain at position 24) and conjugation (between neighboring hydroxyl groups at positions 2 and 3, 20 and 22). Hydroxyl groups could be modified by different chemical moieties, such as polar glycosides, phosphates, sulfates or nonpolar esters, ethers, acetates and benzoates [11,[28][29][30][31]. Table 1 compares values of partition coefficients between polar and non-polar organic solvents for selected polar (20-hydroxyecdysone, α-ecdysone) and less polar (2-deoxydysone and 2,22-dideoxydysone) ecdysteroids of Rhaponticum carthamoides.…”

Section: Chemical Structure Of Ecdysteroids and Its Influence On Retementioning

confidence: 99%

Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides)

Głazowska

Kamiński

2018

J of Separation Science

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The review presents general principles for choosing optimal conditions for ecdysteroid separation, identification, and isolation using HPLC/TLC techniques in RP, NP‐HILIC or NP modes. Analytics of ecdyteroids pose a still insufficiently resolved problem. Plant‐derived ecdysteroids are a point of interest of pharmaceutical industry and sport medicine due to their postulated adaptogenic and anabolic properties. In insects, ecdysteroids regulate larval transformation. Maral root (Rhaponticum carthamoides, Leuzea carthamoides), traditional Siberian folk‐medicine plant used as stimulant to boost overall health and fitness, is a particularly rich source of a wide variety of phytoecdysteroids. The similarity of molecular structures of ecdysteroids present in its extracts together with high content of unrelated compounds of similar chromatographic characteristics makes optimization of separation, identification and isolation of ecdysteroids a difficult analytical task. In that respect, two‐dimensional separations, two‐dimensional separations, 2D HPLC or 2D TLC, could be of use. For identification, the hyphenated techniques are particularly important. Thus, comprehensive overview of MS spectral parameters of ecdysteroids is provided. Described principles could easily be applied for separation of ecdysteroids in extracts from other sources. They are also useful for development of separation procedures for isolation of ecysteroids in preparative‐scale applications.

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Section: Chemical Structure Of Ecdysteroids and Its Influence On Retementioning

confidence: 99%

Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides)

Głazowska

Kamiński

2018

J of Separation Science

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Development of a new high‐performance liquid chromatography‐mass spectrometric method for the analysis of ecdysteroids in biological extracts

Wainwright

Prescott

Lomas

et al. 1997

Arch Insect Biochem Physiol

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Reversed-phase affinity chromatography of ecdysteroids with boronic acid-containing eluents

Gildengorn

1996

Journal of Chromatography A

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Rapid determination of 20-hydroxyecdysteroids in complex mixtures by solid-phase extraction and mass spectrometry

Cited by 9 publications

References 9 publications

Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides)

Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides)

Development of a new high‐performance liquid chromatography‐mass spectrometric method for the analysis of ecdysteroids in biological extracts

Reversed-phase affinity chromatography of ecdysteroids with boronic acid-containing eluents

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