Rapid determination by reversed-phase high-performance liquid chromatography of Vitamins A and E in infant formulas

Alkaline saponification results in decomposition of tocopherols in milk and ovine blood plasma

“…It is noteworthy that ␥-T and ␤-T have the same chemical formula, differing only in the methyl group substitution pattern, and have similar relative antioxidant activities [4]. In line with previous reports [1,2,11], ␣-T, ␦-T and ␥-T as well as ␣-TAc were used in the study, as the native concentration of ␤-T in foods is usually below the limits of UV or fluorescence detection or significantly lower compared with the concentration of ␣-T and other tocopherols.…”

“…Moreover, RP-HPLC without saponification exhibits applicability for determination of ␣-TAc commonly added to milk or infant formula, as this form of vitamin E is less susceptible to oxidation [1,11]. Obviously, methods of analyzing vitamin E without the saponification of assayed samples permit quantification of ␣-TAc as well as ␦-T, ␥-T and ␣-T in milk and infant formula also using reversed-or normal-phase HPLC with fluorescence or/and UV detection [1,2,11]. Therefore, more research is needed to develop a gentle saponification method for the sensitive and accurate HPLC quantification of ␦-T, ␥-T and ␣-T as well as ␣-TAc in milk, meat and products derived from domestic animals.…”

“…Tocopherols, also known as vitamin E, consist of a group of isoprenoid compounds of plant origin, which were first described as essential micronutrients for normal fertility in rats [1][2][3]. Later studies have established a wide range of physiological functions for this vitamin, e.g.…”

Quantification of selected fat soluble vitamins and carotenoids in infant formula and dietary supplements using fast liquid chromatography coupled with tandem mass spectrometry

“…Different saponification conditions have been tested for the extraction of fat soluble vitamins from infant formulas and the selection of adequate procedure was mainly dependent on the target compounds to be analyzed (Chavez-Servin, Castellote, & Lopez-Sabater, 2006;Escriva et al, 2002;Heudi et al, 2004;Huang et al, 2009;Mendoza et al, 2003). Overnight saponification at room temperature was the preferred extraction procedure for the present method.…”

“…Most methods are based on highperformance liquid chromatography (Escriva, Esteve, Farre, & Frigola, 2002;Gentili et al, 2012;Mendoza, Pons, Bargallo, & Lopez-Sabater, 2003) but only a few were developed for the simultaneous determination of FSVs. In a recent review on methods of FSV analysis, the need for developing analytical methods to identify and quantify several vitamins and related compounds such as carotenoids in a single chromatographic run was emphasized (Blake, 2007).…”