Rapid determination and conversion study of 5-hydroxymethylfurfural and its derivatives in glucose injection

Wang, Cheng; Hou, Yajing; Lin, Yuanyuan; Xie, Yitong; Wu, Di; Zhou, Nan; He, Huaizhen

doi:10.1039/c8nj03019g

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…Compounds II and III were prepared in our previous studies and confirmed by MS, 1 H NMR, and 13 C NMR. Their purities were analyzed by high‐performance liquid chromatography (>95%) (Wang et al, 2018). Dulbecco's modified eagle medium (DMEM) was purchased from Invitrogen (California, USA), fetal bovine serum was purchased from Hyclone (Utah, USA), and glutamine, nonessential amino acids, and sodium pyruvate were purchased from Invitrogen (California, USA).…”

Section: Methodsmentioning

confidence: 99%

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5‐Hydroxymethylfurfural multimers induce pseudo‐allergic reaction through FcεRI at high doses

Hou

et al. 2023

J of Applied Toxicology

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Under acidic and high temperature conditions, 5‐hydroxymethylfurfural (5‐HMF) converted from sugar further produces dimers (Compound II) and trimers (Compound III). The polymers were less reported, and sensitization effect of them was reported in this study. Compounds II and III induced the local and systemic anaphylaxis effect in passive cutaneous anaphylaxis mice model and activated RBL‐2H3 cell inducing [Ca2+] mobilization, resulting in the release of β‐hexosaminidase and histamine in vitro. The gene knockdown assay figured out that Compounds II and III induced degranulation through FcεRI. Further, Compounds II and III had a certain affinity with FcεRI by cell membrane chromatography and may combine on the “proline sandwich” structure indicated by molecular docking. All above suggested Compounds II and III can induce pseudo‐allergic reaction through FcεRI in vivo and in vitro. Our work provides basic research to prove that the newly discovered 5‐HMF transformants, Compounds II and III, induce pseudo‐allergic reaction in vitro and in vivo through FcεRI, which is different pathway from 5‐HMF. In foods with high sugar content, the sensitization of Compounds II and III needs more attention. In high‐sugar foods and medicines, especially traditional Chinese medicine injections, the content of transformants needs to be detected.

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Section: Methodsmentioning

confidence: 99%

“…However, preliminary research by our group found that 5‐HMF present in glucose injection can be further converted into dimer (Compound II) and trimer (Compound III) under high temperature and high pressure, especially under acidic conditions (Wang et al, 2018). The autoclaving step of injection makes the appearance of polymers possible.…”

Section: Introductionmentioning

confidence: 99%

5‐Hydroxymethylfurfural multimers induce pseudo‐allergic reaction through FcεRI at high doses

Hou

et al. 2023

J of Applied Toxicology

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“…A weak absorbance was observed in glucose vortexed in 9 mol L −1 H 2 SO 4 , and glucose vortexed for 15 s in 6 mol L −1 H 2 SO 4 displayed UV spectra similar to those of unhydrolyzed glucose, remaining transparent. The peaks in UV spectra indicate the formation of polymerized HMF and other derivatives, such as 5,5′‐(oxydimethylene)‐di‐2‐furaldehyde (Gandini 1997; Wang et al 2018). This dehydration potentially occurred in some published protocols, such as those involving pretreatment with 12 mol L −1 H 2 SO 4 at room temperature for 2 h, followed by incubation at 100°C for 3 h in 1.2 mol L −1 H 2 SO 4 , as described by Pakulski and Benner (1992).…”

Section: Assessmentmentioning

confidence: 99%

An improved method to quantify bulk carbohydrate in marine planktonic samples

Hu,

Irwin,

Finkel

2024

Limnology & Ocean Methods

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The TPTZ (2,4,6‐tripyridyl‐s‐triazine) method is used to detect monosaccharides from seawater and particulate matter samples because it is sensitive, precise, rapid and easy to perform. Contrary to mechanisms proposed in the literature, we provide evidence that the TPTZ method detects hydroxyl as well as aldehyde groups in monosaccharides when all reducing groups are fully deprotonated in alkaline medium. We use this insight to develop an optimized hydrolysis protocol to increase yields from polysaccharides while minimizing the dehydration of monosaccharides. Compared to the TPTZ method with commonly used hydrolysis protocols and the often‐used phenol–sulfuric acid method, our new optimized method detects a wider range of carbohydrates with a more consistent yield relative to glucose and much lower coefficient of variation. When applied to phytoplankton cultures and marine particulate samples, our new method achieves significantly higher bulk carbohydrate yields.

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HMF causes anaphylactic symptoms by acting as a H1 receptor agonist

Hou

et al. 2020

Biochemical Pharmacology

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Rapid determination and conversion study of 5-hydroxymethylfurfural and its derivatives in glucose injection

Abstract: 5-HMF and two identified additional derivatives (II, III) in glucose injection were rapidly separated in less than 7 min for the first time by our normal phase high performance liquid chromatography. The results were more precise than the method adopted by pharmacopeias.

Cited by 8 publications

References 26 publications

5‐Hydroxymethylfurfural multimers induce pseudo‐allergic reaction through FcεRI at high doses

5‐Hydroxymethylfurfural multimers induce pseudo‐allergic reaction through FcεRI at high doses

An improved method to quantify bulk carbohydrate in marine planktonic samples

HMF causes anaphylactic symptoms by acting as a H1 receptor agonist

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