Rapid Detection of Trichodysplasia Spinulosa-Associated Polyomavirus in Skin Biopsy Specimen

Urbano, Paulo Roberto Palma; Pannuti, Cláudio Sérgio; Pierrotti, Lı́gia Camera; David‐Neto, Elias; Romano, Camila Malta

doi:10.1128/genomea.00694-14

Cited by 5 publications

(6 citation statements)

References 6 publications

(6 reference statements)

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“…We describe here the clinical outcome of a case of TS after kidney transplantation and TSPyV DNA detection in blood and urine samples before the development of the skin lesions. A complete reported of genome of TSPyV in this patient has already been published …”

Section: Introductionmentioning

confidence: 99%

Viremia and viruria of trichodysplasia spinulosa‐associated polyomavirus before the development of clinical disease in a kidney transplant recipient

Pierrotti

Urbano

Nali

et al. 2019

Transplant Infectious Dis

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Trichodysplasia spinulosa (TS) is a rare disease associated with immunosuppression and induced by a polyomavirus denominated Tricodisplasia Polyomavirus (TSPyV). We report a case of TS 6 months after kidney transplantation in a 65 years-old woman under immunosuppression therapy with prednisone, mycophenolate and tacrolimus. The patient developed follicular papules on the face with a thickening of the skin and alopecia of the eyebrows, leading to distortion of the face and a leonine appearance characteristic of the disease. The skin biopsy confirmed the clinical diagnosis and the presence of TSPyV DNA in the skin was detected. Staining for SV40 was positive. Immunosuppression was changed: mycophenolate was withdrawn, tacrolimus reduced and everolimus added. Intravenous cidofovir and later on leflunomide were added. Although the literature has reported clinical success with topical cidofovir, we were unable to use it because this drug is not available. There was an improvement of skin lesions and on cosmetic appearance. The patient had three rejections (one clinically diagnosed and two other biopsy proven), progressed with renal failure and graft loss. Retrospective analysis of stored urine and blood samples detected TSPyV DNA in some of those samples two months before the TS clinical development. This case highlights the TSPyV detection in blood and urine samples before the development of skin lesions. K E Y W O R D S kidney transplant, polyomavirus, trichodysplasia spinulosa, viremia, viruria

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Section: Introductionmentioning

confidence: 99%

Viremia and viruria of trichodysplasia spinulosa‐associated polyomavirus before the development of clinical disease in a kidney transplant recipient

Pierrotti

Urbano

Nali

et al. 2019

Transplant Infectious Dis

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“…BKPyV is a member of the Polyomaviridae, which among others contains 13 species detected in humans, including human polyomavirus 9 (HPyV9) and trichodysplasia spinulosa polyomavirus (TSPyV). TSPyV primary infection causes trichodysplasia spinulosa in immunocompromised patients, in whom it can be found in large quantities in affected skin and blood, but also in kidney tissue and urine . TSPyV seroprevalence in healthy populations is high, approximately 75%, which suggests that asymptomatic persistent TSPyV infection is common.…”

Section: Introductionmentioning

confidence: 99%

“…TSPyV primary infection causes trichodysplasia spinulosa in immunocompromised patients, in whom it can be found in large quantities in affected skin and blood, but also in kidney tissue and urine. [9][10][11][12][13] TSPyV seroprevalence in healthy populations is high, approximately 75%, 1,14 which suggests that asymptomatic persistent TSPyV infection is common.…”

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Impact of HPyV9 and TSPyV coinfection on the development of BK polyomavirus viremia and associated nephropathy after kidney transplantation

Rijn

Wunderink

Brouwer

et al. 2019

Journal of Medical Virology

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Background BK polyomavirus (BKPyV) persistently infects the urinary tract and causes viremia and nephropathy in kidney transplantation (KTx), recipients. In a previous study, we observed an increased incidence and load of BKPyV viremia in KTx patients coinfected with human polyomavirus 9 (HPyV9). Here we sought confirmation of this observation and explored whether novel HPyVs that have been detected in urine (HPyV9 and trichodysplasia spinulosa polyomavirus [TSPyV]) potentially aggravate BKPyV infection. Methods A well‐characterized cohort of 209 KTx donor‐recipient pairs was serologically and molecularly analyzed for HPyV9 and TSPyV coinfection. These data were correlated with the occurrence of BKPyV viremia and BKPyVAN in the recipients within a year after KTx. Results Seropositivity for HPyV9 (19%) and TSPyV (89%) was comparable between donors and recipients and did not correlate with BKPyV viremia and BKPyVAN that developed in 25% and 3% of the recipients, respectively. Two recipients developed TSPyV viremia and none HPyV9 viremia. Modification of the predictive effect of donor BKPyV seroreactivity on recipient BKPyV viremia by HPyV9 and TSPyV was not observed. Conclusions Our data provide no evidence for a promoting effect of HPyV9 and TSPyV on BKPyV infection and BKPyVAN in renal allograft patients. Therefore, we do not recommend including HPyV9 and TSPyV screening in KTx patients.

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“…used a set of four primer pairs to amplify and to sequence the complete viral genome, but the authors did not mention which sequencing platform was used. Our group has recently sequenced the whole TSPyV genome from biopsy fragments of spicules using Next Generation Sequencing (NGS) 12 . Lastly, Tsuzuki et al 13 ,.…”

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confidence: 99%

“…Interestingly, all the three lineages include viruses sampled in US, suggesting no geographic clustering of TSPyV. Nevertheless, more comprehensive genetic characterization of this virus needs to be done since only 13 samples were used to classify TSPyV in lineages, and viruses isolated in Brazil 12 , the Netherlands 1 and Japan 14 were not included in this analysis.…”

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confidence: 99%

ALTERNATIVE METHODS FOR SEQUENCING FULL TSPyV GENOMES USING SANGER OR NGS

Urbano

Oliveira

Romano

2016

Rev. Inst. Med. trop. S. Paulo

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Rapid Detection of Trichodysplasia Spinulosa-Associated Polyomavirus in Skin Biopsy Specimen

Abstract: Trichodysplasia spinulosa-associated polyomavirus (TSV) is responsible for a rare skin cancer. Using metagenomic approaches, we determined the complete genome sequence of a TSV first detected in Brazil in spicules of an immunocompromised patient suspected to have trichodysplasia spinulosa.

Cited by 5 publications

References 6 publications

Viremia and viruria of trichodysplasia spinulosa‐associated polyomavirus before the development of clinical disease in a kidney transplant recipient

Viremia and viruria of trichodysplasia spinulosa‐associated polyomavirus before the development of clinical disease in a kidney transplant recipient

Impact of HPyV9 and TSPyV coinfection on the development of BK polyomavirus viremia and associated nephropathy after kidney transplantation

ALTERNATIVE METHODS FOR SEQUENCING FULL TSPyV GENOMES USING SANGER OR NGS

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