Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay

Abstract

In developing countries, pesticides such as triazophos (TZP) are widely used in agriculture. However, the misuse TZP poses a threat to human health and the environment due to its high chemical and photochemical stability. In this study, we developed a lateral flow assay to determine TZP in cucumber. We produced anti-TZP monoclonal antibody with half inhibitory concentration of 2.013 ng/mL according to ELISA. Under optimum conditions, the lateral flow assay had TZP cutoff values of 100 ng/mL in 0.01 M PBS and 250 ng/mL in cucumber, and the results could be visualised with the naked eye in 5 min. our findings revealed that lateral flow assay is a rapid and sensitive method for detect TZP in cucumber.

Monoclonal antibody production and development of immunochromatographic strip assays for screening of the herbicide bispyribac-sodium in rice

“…The target BIS was conjugated to carrier proteins using the 1ethyl-3-(3-dimethylaminopropyl) carbodiimide method. 13,14 Fig. 1 shows the synthesis of the immune antigen (BIS-BSA) and the coating antigen (BIS-OVA).…”

Potential of nanobiosensor in sustainable agriculture: the state-of-art

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