Rapid detection of sulfonamide antibiotics residues in swine urine by surface-enhanced Raman spectroscopy

Xu, Lulu; Wu, Ruimei; Geng, Xin; Zhu, Xiaoyu; Xiong, Yao; Chen, Tao; Ai, Shirong

doi:10.1016/j.saa.2021.120570

Cited by 23 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detection is rarely seen in swine urine samples. Only the determination of β-agonists, ractopamine, and sulfonamides, which are the older generation of antibiotics, has been reported previously [ 20 , 21 , 39 ]. Although enrofloxacin is another widely used antibiotic in swine feedstock, there are limited reports on the residues of this antibiotic.…”

Section: Discussionmentioning

confidence: 99%

“…Raman spectra of urine collected from pigs who did not receive antibiotics were obtained for negative control. The control urine was spiked with antibiotics at various concentrations (10,20,50, 100, and 10,000 ppm) and was used as references. After administering enrofloxacin, pig urine was collected on days 1 and 7 for on-farming antibiotic determination.…”

Section: Raman Spectra Of Antibiotics In Pig Urinementioning

confidence: 99%

“…Surface-enhanced Raman spectroscopy has been widely used to detect various trace elements, such as pesticides and antibiotics in foods, agricultural products, and contaminated water [14][15][16][17][18]. The previous studies have proven that SERS can significantly improve the detection limit for many compounds [19][20][21]. However, studies are currently limited to human samples.…”

Section: Introductionmentioning

confidence: 99%

“…All determined concentrations were lower than the minimal requirements for therapeutic drug monitoring, which is as low as 7.5 μg/mL for ceftriaxone. Another study used SERS to detect sulfonamides in pig urine using gold nanoparticles as an enhancing substrate [ 20 ]. Pig urine spiked with ractopamine and identified at concentrations of 0.1–10 mg/mL [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A novel portable Raman scattering platform for antibiotic screening in pig urine

et al. 2023

View full text Add to dashboard Cite

Background and Aim: Public health and food safety are gaining attention globally. Consumer health can be protected from chemical residues in meat by early detection or screening for antibiotic residues before selling the meat commercially. However, conventional practices are normally applied after slaughtering, which leads to massive business losses. This study aimed to use portable surface-enhanced Raman spectroscopy (SERS) equipped with multivariate curve resolution-alternation least squares (MCR-ALS) to determine the concentrations of enrofloxacin, oxytetracycline, and neomycin concentrations. This approach can overcome the problems of business loss, costs, and time-consumption, and limit of detection (LOD). Materials and Methods: Aqueous solutions of three standard antibiotics (enrofloxacin, oxytetracycline, and neomycin) with different concentrations were prepared, and the LOD for each antibiotic solution was determined using SERS. Extracted pig urine was spiked with enrofloxacin at concentrations of 10, 20, 50, 100, and 10,000 ppm. These solutions were investigated using SERS and MCR-ALS analysis. Urine samples from pigs at 1 and 7 days after enrofloxacin administration were collected and investigated using SERS and MCR-ALS to differentiate the urinary enrofloxacin concentrations. Results: The LOD of enrofloxacin, oxytetracycline, and neomycin in aqueous solutions were 0.5, 2.0, and 100 ppm, respectively. Analysis of enrofloxacin spiking in pig urine samples demonstrated the different concentrations of enrofloxacin at 10, 20, 50, 100, and 10,000 ppm. The LOD of spiking enrofloxacin was 10 ppm, which was 10 times lower than the regulated value. This technique was validated for the first time using urine collected on days 1 and 7 after enrofloxacin administration. The results revealed a higher concentration of enrofloxacin on day 7 than on day 1 due to consecutive administrations. The observed concentration of enrofloxacin was closely correlated with its circulation time and metabolism in pigs. Conclusion: A combination of SERS sensing platform and MCR-ALS is a promising technique for on-farming screening. This platform can increase the efficiency of antibiotic detection in pig urine at lower costs and time. Expansion and fine adjustments of the Raman dataset may be required for individual farms to achieve higher sensitivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Raman Spectra Of Antibiotics In Pig Urinementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A novel portable Raman scattering platform for antibiotic screening in pig urine

et al. 2023

View full text Add to dashboard Cite

show abstract

“…And the detection limit of the three pesticides reached 5.58 × 10 −4 , 1.88 × 10 −4 , and 4.72 × 10 −3 µg/mL, respectively. Xu et al 17 used SERS combined with quick, easy, cheap, effective, rugged, safe (QuEChERS) to detect sulfadiazine and sulfathiazole residues in pig urine by optimizing the amount of PSA, graphitized carbon, and C 18 to achieve a good purification effect. The feature peaks at 819 cm −1 and 825 cm −1 were applied to establish quantitative model, and the minimum detected concentration was about 1 mg/L.…”

Section: Introductionmentioning

confidence: 99%

Exploring Surface-Enhanced Raman Spectroscopy (SERS) Characteristic Peaks Screening Methods for the Rapid Determination of Chlorpyrifos Residues in Rice

Xiong

Huang

et al. 2022

Appl Spectrosc

Self Cite

View full text Add to dashboard Cite

Surface-enhanced Raman spectroscopy (SERS) coupled with characteristic peaks screening methods was developed for analyzing chlorpyrifos (CM) pesticide residues in rice. Au nanoparticles (AuNPs) were prepared as Raman signal enhancement. MgSO4, Primary secondary amine (PSA) and C18 were employed to purify the rice extraction. Successive projections algorithm (SPA) was performed to identify the optimal characteristic peaks of CM in rice from full Raman spectroscopy. Support vector machine (SVM) and partial least squares (PLS) were implemented to investigate the quantitative analysis models. The results demonstrated that six raman peaks such as 671, 834, 1016, 1114, 1436 and 1444 cm-1 were selected by SPA and SVM model had better performance using six peaks (only 0.92% of the full spectra variables) with R2p = 0.97, RMSEP = 2.89 and RPD = 4.26. And the experiment time for a sample was accomplished within 10 min. Recovery for 5 unknown concentration samples was 97.45 ∼ 103.96%. And T-test results also displayed no obvious differences between the measured value and the predicted value. The study stated that the SERS combined with characteristic peaks screening methods can be applied to rapidly monitor the chlorpyrifos residue in rice.

show abstract

Nanozyme based on ZIF-8 for the colorimetric detection of sulfonamides in cow milk

Chen

Zhou

Tian

et al. 2022

Analytical Biochemistry

View full text Add to dashboard Cite

Rapid detection of sulfonamide antibiotics residues in swine urine by surface-enhanced Raman spectroscopy

Cited by 23 publications

References 27 publications

A novel portable Raman scattering platform for antibiotic screening in pig urine

A novel portable Raman scattering platform for antibiotic screening in pig urine

Exploring Surface-Enhanced Raman Spectroscopy (SERS) Characteristic Peaks Screening Methods for the Rapid Determination of Chlorpyrifos Residues in Rice

Nanozyme based on ZIF-8 for the colorimetric detection of sulfonamides in cow milk

Contact Info

Product

Resources

About