Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA)

Zwirglmaier, Katrin; Weyh, Maria; Krüger, Christian; Ehmann, Rosina; Müller, Katharina; Wölfel, Roman; Stoecker, Kilian

doi:10.1016/j.jviromet.2021.114083

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…Developments include immunological methods, such as Enzyme Linked Immunosorbent Assays (ELI-SAs), chemiluminescence immunoassays (CLIAs), lateral ow immunoassays (LFIAs), and multiple others. [3][4][5][6][7] Recently, the development of clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics has been used to detect the SARS-CoV-2 virus. 8,9 This method is very promising but is still in an early stage.…”

Section: Introductionmentioning

confidence: 99%

Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy

et al. 2021

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“…Zwirgelmaier et al showed that simultaneous detection of eight swab samples in under 25 min and with a 100% sensitivity was performed using a prototype PCA. The sensitivity of this assay is 100%, and it can detect the viral load of 1600 copies/µL [ 52 ].…”

Section: Biomarkers In Covid-19mentioning

confidence: 99%

Advances in Biosensing Technologies for Diagnosis of COVID-19

et al. 2022

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The COVID-19 pandemic has severely impacted normal human life worldwide. Due to its rapid community spread and high mortality statistics, the development of prompt diagnostic tests for a massive number of samples is essential. Currently used traditional methods are often expensive, time-consuming, laboratory-based, and unable to handle a large number of specimens in resource-limited settings. Because of its high contagiousness, efficient identification of SARS-CoV-2 carriers is crucial. As the advantages of adopting biosensors for efficient diagnosis of COVID-19 increase, this narrative review summarizes the recent advances and the respective reasons to consider applying biosensors. Biosensors are the most sensitive, specific, rapid, user-friendly tools having the potential to deliver point-of-care diagnostics beyond traditional standards. This review provides a brief introduction to conventional methods used for COVID-19 diagnosis and summarizes their advantages and disadvantages. It also discusses the pathogenesis of COVID-19, potential diagnostic biomarkers, and rapid diagnosis using biosensor technology. The current advancements in biosensing technologies, from academic research to commercial achievements, have been emphasized in recent publications. We covered a wide range of topics, including biomarker detection, viral genomes, viral proteins, immune responses to infection, and other potential proinflammatory biomolecules. Major challenges and prospects for future application in point-of-care settings are also highlighted.

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“…The combination of metagenomics detection and nucleic acid amplification techniques can provide clinicians and epidemiologists with new insights [57]. In the future, immunodiagnostic platforms based on S and N can be used in conjunction with nucleic acid amplification tests to significantly improve COVID-19 detection sensitivity at a low cost [58].…”

Section: Virus Detection Techniquesmentioning

confidence: 99%

Progress of Hollow Materials in Diagnosis of Covid-19

Hussain¹,

Shabbir²,

Faizan³

2022

austinjpharmacolther

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Since the outbreak of COVID-19 in Wuhan, China, it has dramatically changed the global geopolitics, economics, and even society standard norms. The present world scenario is changed regarding business, traveling, and education. Rapid global dissemination and the high mortality rate of coronaviruses are the greatest challenges for drug developers. It will be moving forward toward the identification and treatment of emerging coronaviruses with the aid of nanotechnology. The COVID-19 pandemic raised the question of researchers’ capability to manage this dilemma in a short period. In the present review, we described how hallow material could be developed as a pro-drug that shows an excellent therapeutic effect. Hollow nanoparticles that exploration of antiviral or diagnostic agents against emerging coronaviruses. Hollow nanomaterials in vaccine development are essential because hollow nanocomposites are suitable for mimicking viral structures and antigen delivery. A biosensor that generates a signal from a transducer for comparing and analyzing biological conjugates such as cell receptors, antibodies, RNA, DNA, and nucleic acids. Different biosensors, such as graphene-based biosensors, nanoplasmonic sensor chips, nanomaterial biosensors, electrochemical biosensors, dual modality biosensors, and optical biosensors, have several advantages, characteristics, and a wide range of applications, most remarkably in medical treatment and are used for monitoring and diagnosis. This review focuses on modern experimental studies to identify intelligent and innovative bio/nanomaterials and matrices for developing targeted and controlled drug release systems, nanosensors and nanovaccines to combat pathogenic viruses.

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Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA)

Cited by 5 publications

References 25 publications

Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy

Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy

Advances in Biosensing Technologies for Diagnosis of COVID-19

Progress of Hollow Materials in Diagnosis of Covid-19

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