Rapid detection of Salmonella spp. in food by use of the ISO-GRID hydrophobic grid membrane filter

Abstract

A rapid hydrophobic grid-membrane filter (HGMF) method was developed and compared with the Health Protection Branch cultural method for the detection of Salmonella spp. in 798 spiked samples and 265 naturally contaminated samples of food. With the HGMF method, Salmonella spp. were isolated from 618 of the spiked samples and 190 of the naturally contaminated samples. The conventional method recovered Salmonella spp. from 622 spiked samples and 204 unspiked samples. The isolation rates from Salmonella-positive samples for the two methods were not significantly different (94.6% overall for the HGMF method and 96.7% for the conventional approach), but the HGMF results were available in only 2 to 3 days after sample receipt compared with 3 to 4 days by the conventional method.

Rapid and alternative methods for the detection of salmonellas in foods

“…Subsequently, the \elective lysine agar was replaced by the more selective and Ipecific EF-18 agar (Entis and Boleszczuk 1991;Sharpe 1991). Although the hydrophobic property of the I IGMF .rid lines allows the detection of salmonellas in the presence J f a high level of background flora (Entis et al 1982), .olony purification was sometimes required for raw meat and poultry samples (Entis and Roleszczuk 1991). The f IGMF technique has received Association of' Analytical tihemists (AOACJ official first action status (Andrews 1992).…”

Isolation of salmonellas by immunomagnetic separation

“…1978), radiometric methods (Stewart et al. 1980), an iso-grid hydrophobic grid membrane filtration procedure (Entis et al 1982), DNA hybridization techniques (Fitts et al. 1983;Fitts 1985), impedance measurements (Smith et al.…”

Rapid detection of salmonellas in raw meats using a fluorescent antibody‐microcolony technique

“…enrichment serology (Sperber & Deibel 1969), membrane filter-disc immuno-immobilization (Swaminathan et al 1978), radiometry (Stewart et al. 1980), specific bacteriophages (Hirsch & Martin 1984) containing cloned luminescent genes to improve detection (Schutzbank et al 1989), hydrophobic grid membrane filtration methods (Entis et al 1982), DNA-DNA hybridization probes (Fitts et al 1983), various enzyme-linked immunosorbent assays (Minnich et al 1982; Anderson & Hartman 1985;Ibrahim 1986;Clayden et al 1987; Beckers et al 1988;Flowers et al 1988), immuno-latex agglutination tests (Hadfield et al 1987) and conductance/impedance based methods (Easter & Gibson 1985). Most have shown some considerable degrees of success, but none have been widely adopted as yet.…”