Rapid Detection of Salmonella from Poultry by Real-Time Polymerase Chain Reaction with Fluorescent Hybridization Probes

Eyigör, Ayşegül; Çarlı, K. Tayfun

doi:10.1637/0005-2086(2003)047[0380:rdosfp]2.0.co;2

Cited by 38 publications

(29 citation statements)

References 27 publications

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“…Consistent with results of this work, PCR can represent an alternative to costly and time consuming culture methods for the detection of pathogenic Salmonella species in a sample (11)(12)(13). In addition, pre-screening Objetivos.…”

Section: Discussionsupporting

confidence: 76%

Comparison of polymerase chain reaction and bacterial culture for Salmonella detection in the Muscovy duck in Trinidad and Tobago

Rampersad¹,

Johnson²,

Brown³

et al. 2008

Rev Panam Salud Publica

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Objectives. The purpose of this study was to investigate the presence and serovar identity ofPolymerase chain reaction, Salmonella, Trinidad and Tobago. ABSTRACTSerovars of the bacterium Salmonella enterica ssp. enterica are known to be a significant cause of foodborne illness in humans. It is well established that different serovars can be associated with different animals-e.g., S. Enteritidis in poultry-which act as a source of foodborne infection in humans (1). The current strategy for food safety is one that starts on the farm. The "Food Safety from Farm to Table" initiative of the United States Food and Drug Administration's Center for Food Safety and Applied Nutrition (2) and the European Union's "Farm to Fork" integrated approach (3) are two such strategies that underscore the importance of detecting Salmonella in food animals at the farm level.The Muscovy duck (Cairina moschata), and its hybrid mules, are relatively large, hardy birds, raised globally for

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Section: Discussionsupporting

confidence: 76%

Comparison of polymerase chain reaction and bacterial culture for Salmonella detection in the Muscovy duck in Trinidad and Tobago

Rampersad¹,

Johnson²,

Brown³

et al. 2008

Rev Panam Salud Publica

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“…Previous studies have used molecular techniques to identify pathogens and/or genes directly from samples. These methods have proven to be reliable, cost-effective, user friendly, sensitive and specific as well as providing a distinct advantage in regards to time saving over conventional culture methods (Blessmann et al, 2002;Eyigor and Carli, 2003;Garofalo et al, 2007;McOrist et al, 2002).…”

Section: Chapter 1: Literature Reviewmentioning

confidence: 99%

“…For human stool samples, the extraction using the QIAamp DNA Stool Mini Kit (Qiagen) yielded statistically greater PCR sensitivity in comparison to the Chelex methods (Cordova et al, 2010) Overall, molecular methods to detect pathogens or VGs directly from faeces have proven to be reliable, cost-effective, user friendly and accurate in other animal species, therefore, providing a distinct advantage over conventional methods (Blessmann et al, 2002;Eyigor and Carli, 2003;Garofalo et al, 2007;Gioffre et al, 2004;McOrist et al, 2002). However, currently, there is a lack of information pertaining to the quality and quantity of extracted DNA from chicken faecal samples using different DNA extraction methods (Barnard et al, 2011).…”

Section: (B) (A)mentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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“…There may be potential in the future for improving detection and confirmation of Salmonella by the use of molecular methods such as PCR or microarray (Bailey, 1998;Eyigor and Carli, 2003;Fratamico, 2003) but these do not always perform reliably in all laboratories, especially in a faecal matrix, and such alternative methods have to be validated according to European EN/ISO 16140:2003 25 standards.…”

Section: Monitoring Systems In the Eumentioning

confidence: 99%

Scientific Opinion on an estimation of the public health impact of setting a new target for the reduction ofSalmonellain turkeys

Publication¹

2012

EFSA Journal

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The quantitative contribution of turkeys and other major animal‐food sources to the burden of human salmonellosis in the European Union was estimated. A ‘Turkey Target Salmonella Attribution Model’ (TT‐SAM) based on the microbial‐subtyping approach was used. TT‐SAM includes data from 25 EU Member States, four animal‐food sources of Salmonella and 23 Salmonella serovars. The model employs 2010 EU statutory monitoring data on Salmonella in animal populations (EU baseline survey data for pigs), data on reported cases of human salmonellosis and food availability data. It estimates that 2.6 %, 10.6 %, 17.0 % and 56.8 % of the human salmonellosis cases are attributable to turkeys, broilers, laying hens (eggs) and pigs, respectively. The top‐6 serovars of fattening turkeys that contribute most to human cases are S. Enteritidis, S. Kentucky, S. Typhimurium, S. Newport, S. Virchow and S. Saintpaul. Comparing the prevalence of Salmonella in turkey flocks reported in 2010 with a theoretical combined prevalence for S. Enteritidis and S. Typhimurium of 1 % (i.e. the transitional target), a reduction of 0.4 % in the percentage of turkey‐associated human salmonellosis cases would be achieved. However, when adjusting the combined prevalence of all serovars to 1 %, an 83.2 % reduction in the percentage of turkey‐associated human salmonellosis cases, equivalent to 2.2 % of all human salmonellosis cases, is expected. Uncertainty and data limitations are discussed, including recommendations on how these could be overcome. Vertical transmission of Salmonella as well as hatchery acquired Salmonella contamination originating from breeding stock are very important sources for Salmonella infection in turkeys, and therefore controlling Salmonella in breeding flocks as well as in rearing and fattening flocks is necessary to minimise Salmonella in turkeys at slaughter.

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Rapid Detection of Salmonella from Poultry by Real-Time Polymerase Chain Reaction with Fluorescent Hybridization Probes

Cited by 38 publications

References 27 publications

Comparison of polymerase chain reaction and bacterial culture for Salmonella detection in the Muscovy duck in Trinidad and Tobago

Comparison of polymerase chain reaction and bacterial culture for Salmonella detection in the Muscovy duck in Trinidad and Tobago

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Scientific Opinion on an estimation of the public health impact of setting a new target for the reduction ofSalmonellain turkeys

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