Rapid detection of Salmonella enterica in food samples by a novel approach with combination of sample concentration and direct PCR

Vinayaka, Aaydha Chidambara; Ngo, Tien Anh; Kant, Kamal; Engelsmann, Pia; Dave, Vivek Priy; Shahbazi, Mohammad‐Ali; Wolff, Anders; Bang, Dang Duong

doi:10.1016/j.bios.2018.09.078

Cited by 109 publications

(74 citation statements)

References 31 publications

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“…The collection of S. enterica cells using immunomagnetic beads from egg yolk and PCR was also reported by Vinayaka et al [24]. In comparison with the same sampling of S. enterica suspended in buffer solution, egg yolk did not affect PCR and almost the same signal and linearity were observed [24]. In this study, the observed detection limit and detectable range were comparable with the observed result without using egg yolk in our previous study [19].…”

Section: Detection Of S Enterica By Pcr On the Microfluidic Discsupporting

confidence: 90%

“…Thus, a linear relationship between the number of microchambers that exceeded the threshold and the concentration of cells indicates that the proposed procedure, including collection of bacteria using the beads, entrapment of the beads in the microchambers, and PCR, reflects the actual concentration of S. enterica cells. The collection of S. enterica cells using immunomagnetic beads from egg yolk and PCR was also reported by Vinayaka et al [24]. In comparison with the same sampling of S. enterica suspended in buffer solution, egg yolk did not affect PCR and almost the same signal and linearity were observed [24].…”

Section: Detection Of S Enterica By Pcr On the Microfluidic Discsupporting

confidence: 55%

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Detection of Salmonella Enterica in Egg Yolk by PCR on a Microfluidic Disc Device Using Immunomagnetic Beads

Kubo

Kajiya

Aramaki

et al. 2020

Sensors

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Salmonella enterica is a pathogenic bacterium that causes foodborne illness. One of the vehicle foods of S. enterica are chicken eggs. Efficient collection of the bacterium is necessary to detect it specifically. We developed a method to detect S. enterica by PCR on a microfluidic disc device using a fluorescent probe. Salmonella enterica cells were isolated in the microchambers on the device, followed by thermal lysis and PCR targeting with the invA gene, a gene specific to S. enterica, were observed by measurement of the fluorescent signal that resulted from gene amplification. However, the developed method was unable to discriminate viable cells from dead cells. Consequently, in this study, magnetic beads modified with anti-Salmonella antibody were utilized to detect viable Salmonella cells from egg yolk prior to PCR on the device. While using the antibody-modified beads, egg yolk components, which inhibit PCR, were removed. The collected cells were subsequently detected by PCR of the invA gene on a microfluidic disc device. This method enabled the detection of viable cells without the inhibition of PCR by any egg component. S. enterica was detected at 5.0×104 cells mL−1 or at a higher concentration of egg yolk within 6 h including the sampling time.

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Section: Detection Of S Enterica By Pcr On the Microfluidic Discsupporting

confidence: 90%

Section: Detection Of S Enterica By Pcr On the Microfluidic Discsupporting

confidence: 55%

Detection of Salmonella Enterica in Egg Yolk by PCR on a Microfluidic Disc Device Using Immunomagnetic Beads

Kubo

Kajiya

Aramaki

et al. 2020

Sensors

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“…Immunomagnetic separation (IMS) is effective for identifying bacteria from foods due to selective separating target molecules from a heterogeneous matrix [12][13][14][15]. A target-specific antibody on the surface of a magnetic bead is a crucial factor for increasing the yield of selective separation and the concentration of the antigenic target [16][17][18]. Especially, the magnetic beads could be successfully separated and concentrated from the large volume of the sample solution by applying the external magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Park

et al. 2020

Sensors

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The food industry requires rapid and simple detection methods for preventing harm from pathogenic bacteria. Until now, various technologies used to detect foodborne bacteria were time-consuming and laborious. Therefore, we have developed an automated immunomagnetic separation combined with a colorimetric assay for the rapid detection of E. coli O157:H7 in food samples. The colorimetric detection method using enzymatic reaction is fascinating because of its simplicity and rapidity and does not need sophisticated devices. Moreover, the proposed procedures for the detection of bacteria in food take less than 3 h including pre-enrichment, separation and detection steps. First, target-specific immunomagnetic beads were introduced to contaminated milk in a pre-enrichment step. Second, the pre-enriched sample solution containing target bacteria bound on immunomagnetic beads was injected into an automated pretreatment system. Subsequently, the immunomagnetic beads along with target bacteria were separated and concentrated into a recovery tube. Finally, released β-galactosidase from E. coli O157:H7 after lysis was reacted with chlorophenol red β-galactopyranoside (CPRG) used as a substrate and the colorimetric change of CPRG was determined by absorbance measuring or the naked eye. By the proposed approach in this study, we could detect 3 × 102 CFU/mL of E. coli O157:H7 from a milk sample within 3 h.

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“…Various methods have been described by other groups offering fast and easily applicable multi‐analysis solutions for the detection of bacterial pathogens. Vinayaka et al . developed integrated immuno‐magnetic PCR and detected S. typhimurium directly from foods.…”

Section: Discussionmentioning

confidence: 99%

“…Various methods have been described by other groups offering fast and easily applicable multi-analysis solutions for the detection of bacterial pathogens. Vinayaka et al 37 developed integrated immuno-magnetic PCR and detected S. typhimurium directly from foods. Through a combination of immuno-magnetic beads, their approach enabled rapid and sensitive measurement of S. typhimurium at concentrations as low as 2-3 CFU mL −1 within 3 h. Wang et al 38 designed a biosensor for rapid, sensitive and online monitoring of S. typhimurium using immuno-magnetic separation, fluorescent labeling, a microfluidic chip and smartphone video processing.…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of foodborne bacterial pathogens using visual high‐throughput microfluidic chip

Jin

Ding²,

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND Foodborne diseases are a major global public health concern. It is urgent to monitor foodborne pathogens from contaminated foods based on multi‐target detection. RESULTS In this work, a self‐priming compartmentalization (SPC) microdevice made of poly(methyl methacrylate) (PMMA) was developed and integrated into a loop‐mediated isothermal amplification (LAMP) system for performing high‐throughput visual detection. The microfluidic chip is a self‐filling microwell array which can introduce reaction solution only through an access hole in a vacuum pumping system. The independent and closed wells can avoid cross‐contamination and ensure the accuracy of the results. The approach was able to perform the simultaneous identification of six foodborne pathogens (Salmonella typhimurium, Bacillus cereus, Staphylococcus aureus, Yersinia enterocolitica, Vibrio parahaemolyticus and Listeria monocytogenes) within 1 h. The results demonstrated that the method could specifically recognize the six foodborne pathogens. CONCLUSION The use of a LAMP‐based microdevice can be a faster alternative to conventional methods to detect pathogens in food samples, especially for those foods in which no pathogen is allowed (S. aureus), or foods for infant use. The simplicity and portability of the device can provide an alternative platform for foodborne pathogens where food safety needs accurate and timely detection confirmation. © 2020 Society of Chemical Industry

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Rapid detection of Salmonella enterica in food samples by a novel approach with combination of sample concentration and direct PCR

Cited by 109 publications

References 31 publications

Detection of Salmonella Enterica in Egg Yolk by PCR on a Microfluidic Disc Device Using Immunomagnetic Beads

Detection of Salmonella Enterica in Egg Yolk by PCR on a Microfluidic Disc Device Using Immunomagnetic Beads

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Rapid detection of foodborne bacterial pathogens using visual high‐throughput microfluidic chip

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