Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay

Beenhouwer, H. De; Lhiang, Z.; Jannes, Geert; Mijs, Wouter; Machtelinckx, L.; Rossau, R.; Traore, Hamidou; Portaels, F.

doi:10.1016/0962-8479(95)90009-8

Cited by 188 publications

(119 citation statements)

References 21 publications

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“…Several other genotypic methods have been proposed to detect resistance to antibiotics, such as PCR-single strand conformation polymorphism [57], PCR-heteroduplex formation [62], and solid-phase hybridisation assays. Moreover, the Line Probe Assay (LiPA-Rif) based on the hybridisation of amplified DNA from cultured strains or clinical samples to ten probes encompassing the core region of the rpoB gene of M. tuberculosis, immobilised on a nitrocellulose strip [63], and the GenoType mycobacterium tuberculosis test (MTB) DR (Hain, Germany), a commercial system for the detection of the M. tuberculosis complex and its resistance to rifampicin and isoniazid from culture samples based on the detection of the most common mutations in the rpoB and katG genes, respectively. The LiPA-Rif assay has been evaluated in different settings giving encouraging results [64].…”

Section: Genotypic Methodsmentioning

confidence: 99%

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Palomino¹

2005

European Respiratory Journal

144

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Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Of great concern for TB control is the emergence of drug resistance. Since there is no cure for some multidrug-resistant strains of Mycobacterium tuberculosis, there is concern that they may spread around the world, stressing the need for additional control measures, such as new diagnostics, better drugs for treatment, and a more effective vaccine.Pulmonary TB can be diagnosed by its symptoms, chest radiography, sputum smear microscopy and by cultivation of M. tuberculosis, which is considered as the gold standard. Recent advances in molecular biology and molecular epidemiology, and a better understanding of the molecular basis of drug resistance in TB, have provided new tools for rapid diagnosis; however, the high cost of most of these techniques, and their requirement for sophisticated equipment and skilled personnel have precluded their implementation on a routine basis, especially in low-income countries.Other nonconventional diagnostic approaches proposed include the search for biochemical markers, detection of immunological response and early detection of M. tuberculosis by methods other than colony counting.In the present article, some of these approaches will be reviewed and the feasibility for their implementation in diagnostic laboratories will be discussed.

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Section: Genotypic Methodsmentioning

confidence: 99%

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Palomino¹

2005

European Respiratory Journal

144

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“…20 Benefits of this technique include the rapid speed with which rifampicin resistance can be detected, normally within 48 hours. 21 Although the test has high sensitivity and specificity when used on culture isolates, it has lower sensitivity when used directly on clinical specimens. 22 Additionally, it has been suggested that the reliability of the test in acting as a surrogate marker for MDR TB will depend on the prevalence of rifampicin resistance in the population.…”

Section: Line Probe Assaysmentioning

confidence: 99%

Clinical implications of the global multidrug-resistant tuberculosis epidemic

Kumar

Abubakar

2016

Clinical Medicine

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Multidrug-resistant tuberculosis (MDR TB) IntroductionThe significant threat of the global multidrug-resistant tuberculosis (MDR TB) epidemic has been described as a crisis in the World Health Organization's (WHO's) End TB strategy .1 MDR TB is defined as tuberculosis (TB) caused by Mycobacterium tuberculosis isolates that are resistant to at least both rifampicin and isoniazid.2 The WHO's Global tuberculosis report supplement in 2014 estimated that MDR TB accounts for 3.5% (95% confidence interval (CI) 2.2-4.7%) of all new cases of TB and 20.5% (95% CI 13.6-27.5%) of TB cases among individuals previously treated for the disease. 3 There were approximately 480,000 new cases of MDR TB globally in 2013, with an estimated 210,000 deaths attributable to MDR TB. The highest levels of MDR TB are in Eastern European and Asian countries, with more than half the global burden of MDR TB being located in India, China and Russia. 4 Additional cause for concern is the emergence of extensively drug-resistant TB (XDR TB), which is defined as tuberculosis caused by M tuberculosis isolates that are resistant to rifampicin, isoniazid, any ABSTRACT fluoroquinolones and at least one of the second-line injectable drugs (capreomycin, kanamycin and amikacin). Here we provide an overview of the importance of MDR TB, its epidemiology, clinical implications, potential ways of addressing the disease and prospects for new diagnostics and treatment. EpidemiologyGlobal epidemiology of TB

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“…Some approaches like PCR amplification followed by hybridization are easy to apply. One such assay is commercially marketed by Inno-Genetics [41]. While this assay has been found to be reproducible and promising, it is not applicable to a number of Indian strains which have novel mutations.…”

Section: Pcr Technology For Diagnosis Of Tuberculosisindian Scenariomentioning

confidence: 99%

Advances in Molecular Diagnosis of Tuberculosis

Katoch

2003

Medical Journal Armed Forces India

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Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay

Cited by 188 publications

References 21 publications

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Clinical implications of the global multidrug-resistant tuberculosis epidemic

Advances in Molecular Diagnosis of Tuberculosis

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