Rapid detection of Rh(D)‐ or K‐positive fetal red cells in chorion villus samples by a flow cytometric technique

Nelson, Margaret; Forsyth, Cecily; Popp, H; Gibson, John

doi:10.1111/j.1365-3148.1994.tb00267.x

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…Once previously sensitized patients who were carrying Rh-negative fetuses were removed from group 1, the overall success rate for all three groups was 93.4% accurate in assigning the outcome to either moderately or severely affected by HDN or unaffected. It would be hoped that in future those women already highly sensitized carrying Rh-negative fetuses could be identified by PCR [17] or flow cytometry [18]. The CL assay could distinguish between patients that were unaffected or only required phototherapy and those suffering HDN that required transfusion therapy.…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Study of Red Cell Maternal Antibodies by Chemiluminescence

et al. 1997

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Plasma samples from 109 patients with maternal IgG alloantibodies were investigated using a chemiluminescence (CL) assay, a functional test, to predict which antibodies were clinically significant. The CL assay was able to distinguish between those patients who were unaffected or mildly affected requiring only phototherapy, and those patients with moderate or severe haemolytic disease of the fetus or newborn (HDN) requiring transfusion therapy. The CL result was compared with the anti-D quantification result, the number of IgG molecules bound per red cell and, in 80% of the cases, the monocyte monolayer assay. If mothers carrying Rh-negative fetuses were ignored, then the CL assay correctly predicted the outcome for 93.4% of all cases (including those other than D), while the AutoAnalyzer and monocyte assay predicted correctly 92.7% (of the anti-D patients) and 81.5%, respectively. Greater than 80% of patients with severe or moderate HDN had both IgG1 + IgG3 subclasses in the maternal plasma, while those infants who were unaffected or only mildly affected had a greater chance of having IgG1 only (44%) in the maternal plasma, IgG3 only (27%) or both subclasses (29%).

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Section: Discussionmentioning

confidence: 99%

A Retrospective Study of Red Cell Maternal Antibodies by Chemiluminescence

et al. 1997

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show abstract

“…Once previously sensitized patients who were carrying Rh-negative fetuses were removed from group 1, the overall success rate for all three groups was 93.4% accurate in assigning the outcome to either moder ately or severely affected by HDN or unaffected. It would be hoped that in future those women already highly sensitized carrying Rh-negative fetuses could be identified by PCR [17] or flow cytometry [18]. The CL assay could distinguish between patients that were unaffected or only required pho totherapy and those suffering HDN that required transfu sion therapy.…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Study of Red Cell Maternal Antibodies by Chemiluminescence

Downing¹,

Bromilow²,

Templeton³

et al. 1996

Vox Sanguinis

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Plasma samples from 109 patients with maternal IgG alloantibodies were investigated using a chemiluminescence (CL) assay, a functional test, to predict which antibodies were clinically significant. The CL assay was able to distinguish between those patients who were unaffected or mildly affected requiring only phototherapy, and those patients with moderate or severe haemolytic disease of the fetus or newborn (HDN) requiring transfusion therapy. The CL result was compared with the anti-D quantification result, the number of IgG molecules bound per red cell and, in 80% of the cases, the monocyte monolayer assay. If mothers carrying Rh-negative fetuses were ignored, then the CL assay correctly predicted the outcome for 93.4% of all cases (including those other than D), while the AutoAnalyzer and monocyte assay predicted correctly 92.7% (of the anti-D patients) and 81.5%, respectively. Greater than 80% of patients with severe or moderate HDN had both IgGl+lgG3 subclasses in the maternal plasma, while those infants who were unaffected or only mildly affected had a greater chance of having IgGl only (44%) in the maternal plasma, IgG3 only (27%) or both subclasses (29%).

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“…These include the rosette screening test 2 as well as immunofluorescence flow cytometry (IFC). [3][4][5] Several alternative strategies for flow cytometric measurements of FMHs of fetal D+ red blood cells (RBCs) in D-mothers have been described. These include (1) indirect staining, generally using an FITC-conjugated Fab preparation of anti-human IgG to detect RBC-bound anti-D after incubating the maternal RBC sample with IgG anti-D; 3 pared in order to provide a calibration curve, were also incubated with FITC-BRAD 3.…”

Section: Methodsmentioning

confidence: 99%

Quantitating fetomaternal hemorrhages of D+ red cells using an FITC-conjugated IgG monoclonal anti-D by flow cytometry: a case report

Lubenko¹,

Collier

Hindmarch

et al. 1997

Immunohematology

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Several methods for quantitating fetomaternal hemorrhages (FMHs) have been described; these include the Kleihauer-Betke and red cell rosetting tests, and flow cytometry that uses an indirect antiglobulin technique, employing either FITC-conjugated IgG/unlabeled anti-D or streptavidin conjugates with biotinylated anti-D to enumerate D+ red cells in maternal blood. We have used a recently described directly conjugated FITC anti-D for direct flow cytometric (direct FC) quantitation of FMH in a patient who presented with a large fetal bleed (approx. 80mL) as determined using the Kleihauer method. We compared the efficacy of the direct FC technique to the roset- ting and Kleihauer tests in estimating the quantity of Rh immunoglobulin (RhIg) to be administered to the mother to suppress Rh alloimmunization. Both the Kleihauer and the direct FC gave precise estimates of 80mL for the size of bleed, whereas the rosetting test failed to be as precise. The former tests predicted that a 10,000 iu dose (2,000 μ g) of RhIg would be adequate; the lack of alloanti-D in a maternal follow-up sample obtained 9 months after delivery supported this prediction and underlined the reliability of the direct FC method as an alternative to the Kleihauer method for quantitating large FMHs. Immunohematology 1997;13:12–14.

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Rapid detection of Rh(D)‐ or K‐positive fetal red cells in chorion villus samples by a flow cytometric technique

Cited by 10 publications

References 16 publications

A Retrospective Study of Red Cell Maternal Antibodies by Chemiluminescence

A Retrospective Study of Red Cell Maternal Antibodies by Chemiluminescence

A Retrospective Study of Red Cell Maternal Antibodies by Chemiluminescence

Quantitating fetomaternal hemorrhages of D+ red cells using an FITC-conjugated IgG monoclonal anti-D by flow cytometry: a case report

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