Rapid Detection of Respiratory Tract Viral Infections and Coinfections in Patients with Influenza-Like Illnesses by Use of Reverse Transcription-PCR DNA Microarray Systems

Renois, Fanny; Talmud, Déborah; Huguenin, A; Moutte, Lauryane; Strady, C.; Cousson, J.; Lévêque, Nicolas; Andréoletti, Laurent

doi:10.1128/jcm.00733-10

Cited by 83 publications

(112 citation statements)

References 39 publications

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“…Farklı solunum viruslarıyla enfekte hastalar benzer klinik sendromlara sahip olduğundan, etiyolojik etkenin özgül olarak tanımlanması ancak laboratuvar çalışmalarıyla mümkündür. Günümüzde polimeraz zincir reaksiyonu (PCR) temelli testler, solunum yolu viruslarının tanımlanmasında, konvansiyonel yöntemlerden (virus izolasyonu ve antijen testleri) daha hızlı ve duyarlı sonuç vermektedir [6][7][8] . Özellikle çok sayıdaki solunum virusunu tek bir örnekte aynı anda tespit edebilen multipleks PCR/mikroarray yöntemlerinin kullanıma girmesi, gerek viral koenfeksiyonların prevalansının belirlenmesinde, gerekse doğru ve etkili antiviral tedaviye karar verilmesinde büyük yarar sağlamıştır 4,[8][9][10] .…”

Section: Introductionunclassified

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

Uyar¹,

Kuyucu²,

Tezcan³

et al. 2014

Mikrobiyol Bul

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* Bu çalışma, Mersin Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir (Proje no. TF DTB [MU] 2010-4 TU). ÖZETAkut bronşiyolit, daha çok iki yaşından küçük çocuklarda sıklıkla viral etkenlerin neden olduğu bir alt solunum yolu enfeksiyonudur. Bu çalışmada, 0-2 yaş arası akut bronşiyolitli çocuklarda, insan bokavirusu (HBoV) da dahil olmak üzere solunum yolu viruslarının sıklığının belirlenmesi ve bu etkenlerle oluşan enfeksiyonların klinik özelliklerinin değerlendirilmesi amaçlanmıştır. Çalışmaya, Ocak-Haziran 2010 tarihleri arasında Mersin Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalında akut bronşiyolit tanısı alan 62 hasta (45 erkek, 17 kız; yaş aralığı: 0-2 yıl) ile kontrol grubu olarak sağlıklı 33 çocuk (21 erkek, 12 kız; yaş aralığı: 0-2 yıl) dahil edilmiştir. Çalışma gruplarından alınan nazofarengeal aspirat örneklerinde solunum yolu virusları [respiratuar sinsityal virusu (RSV) A ve B; rhinovirus (RV); insan metapnömovirusu (hMPV) A ve B; influenza virus tip A (H1N1, H3N2, H1N1v), B ve C; parainfluenza virus (PIV) tip 1, 2, 3 ve 4A/B; adenovirus (AdV); HBoV; koronavirus (CoV 229E); enterovirus (EV)], multipleks polimeraz zincir reaksiyonu (M-PCR) ve DNA mikroarray prensibi ile çalışan CLART ® Pneumovir (Clinical Array Technology, Genomica, İspanya) ticari sistemi ile araştırılmıştır. Ayrıca, viral etkenler ile hastaların demografik, klinik ve laboratuvar özellikleri, uygulanan tedaviler ve hastanede yatış oranları arasındaki ilişki değerlendirilmiştir. Çalışmamızda, akut bronşiyolitli 62 hastanın 52 (%83.9)'sinde en az Geliş Tarihi (Received): 12.12.2013 • Kabul Ediliş Tarihi (Accepted): 11.04.2014

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Section: Introductionunclassified

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

Uyar¹,

Kuyucu²,

Tezcan³

et al. 2014

Mikrobiyol Bul

View full text Add to dashboard Cite

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“…The high-density microarray, which has not been broadly applied in routine clinical diagnosis, can be used to test thousands of potential known or unknown pathogens. The low-density microarray analytic technology was rapidly developed by clinical specimen tests based on multiplex RT-PCR amplification [13][14][15]. Some low-density chips have been certified by CE Mark and applied to the in vitro diagnosis of human viral diseases, as viruses can be quickly detected using this method.…”

Section: Discussionmentioning

confidence: 99%

“…Some low-density chips have been certified by CE Mark and applied to the in vitro diagnosis of human viral diseases, as viruses can be quickly detected using this method. The effective diagnosis of patients in hospitals with infectious diseases can be improved, and epidemiological surveys on infectious pathogens can be performed using this microarray technique [8,13,15].…”

Section: Discussionmentioning

confidence: 99%

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Microarray Multiplex Assay for the Simultaneous Detection and Discrimination of Influenza A and Influenza B Viruses

Tian

et al. 2013

Indian J Microbiol

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In this study, we present a microarray approach for the typing of influenza A and B viruses, and the subtyping of H1 and H3 subtypes. We designed four pairs of specific multiplex RT-PCR primers and eight specific oligonucleotide probes and prepared microarrays to identify the specific subtype of influenza virus. Through amplification and fluorescent marking of the multiplex RT-PCR products on the M gene of influenza A and B viruses and the HA gene of subtypes H1 and H3, the PCR products were hybridized with the microarray, and the results were analyzed using a microarray scanner. The results demonstrate that the chip developed by our research institute can detect influenza A and B viruses specifically and identify the subtypes H1 and H3 at a minimum concentration of 1 9 10 2 copies/lL of viral RNA. We tested 35 clinical samples and our results were identical to other fluorescent methods. The microarray approach developed in this study provides a reliable method for the monitoring and testing of seasonal influenza.

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“…Several studies have reported that dual or multiple respiratory viral infections may influence the severity of disease in children (2,8). The incidence of viral respiratory co-infections is often reported between 10% and 20% (9,10).…”

Section: Introductionmentioning

confidence: 99%

Detection of Respiratory Co-Infections in Children Less Than Five Years With Adenovirus Infection

Malekshahi

Shafiei-Jandaghi

Yavarian

et al. 2016

Arch Pediatr Infect Dis

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Background: Acute respiratory tract infection is associated with significant morbidity, mortality, and economic loss worldwide. Viral infections seem to be responsible for 80% of the cases. There are several reports on the influence of dual or multiple respiratory viral infections on the severity of disease in childhood. Objectives: A limited number of studies have been conducted on co-infection of Adenovirus (AdV) with coxsackievirus, human bocavirus (HBoV) and Mycoplasma pneumoniae (MP) in Iran. To address this, the present article focuses on both the etiology and epidemiology of multiple microbial respiratory infections (coxsackievirus, HBoV, MP and influenza virus) and their clinical significance in young Iranian children with confirmed AdV infection. Patients and Methods: Molecular detection of HBoV, coxsackievirus, MP and influenza virus was performed by conventional PCR in 71 respiratory adenovirus-positive samples obtained from children younger than 5 years of age. Results: Among the 71 adenovirus-positive samples, 6 (8.4%) were co-infected, three were co-infected with MP and three of which were co-infected with influenza A/H3N2. Of the six patients with co-infection, four were male and two were female; three patients were less than 1 year of age and the remaining were 2, 3 and 4 years of age. Moreover, there were two inpatients and four outpatients. Conclusions: Although several studies have investigated viral respiratory co-infection, no study has evaluated the rate of respiratory co-infections in adenovirus-positive samples from children younger than 5 years. However, this study has filled this gap, the the number of co-infections were too small to draw any definite conclusions. Therefore, large-scale studies using bigger samples are required to understand the clinical significance of polymicrobial acute respiratory infections.

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Rapid Detection of Respiratory Tract Viral Infections and Coinfections in Patients with Influenza-Like Illnesses by Use of Reverse Transcription-PCR DNA Microarray Systems

Cited by 83 publications

References 39 publications

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

Microarray Multiplex Assay for the Simultaneous Detection and Discrimination of Influenza A and Influenza B Viruses

Detection of Respiratory Co-Infections in Children Less Than Five Years With Adenovirus Infection

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