Rapid detection of `rare' actinomycetes in environmental samples

Gathogo, Esther; Waugh, Amy; Perić-Concha, Nataša; Redpath, Maria B.; Long, Paul F.

doi:10.1023/b:bile.0000025899.96174.7d

Cited by 3 publications

(2 citation statements)

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“…We believe that our screening strategy should increase the possibility of discovering bioactive molecules from rare actinobacteria (Gathogo et al 2004). The isolation of the marine actinobacterium is a significant finding as it is the first marine bacterial source producing a single compound active against MDR bacteria and leukemia cells.…”

Section: Resultsmentioning

confidence: 99%

Production and Purification of a Bioactive Substance Inhibiting Multiple Drug Resistant Bacteria and Human Leukemia Cells from a Salt-Tolerant Marine Actinobacterium sp. Isolated from the Bay of Bengal

et al. 2006

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Four marine actinobacteria tolerant to 200 g NaCl l(-1) were screened for antibacterial activity against eight patient-derived multiple drug resistant (MDR) bacteria. The active compound (MW 300.2, predicted molecular formula C(20)H(28)O(2)) from an actinobacterium, was inhibitory to three Gram-positive and three Gram-negative MDR bacteria, seven non-clinical Gram-positive, four Gram-negative bacteria and five fungi (MIC: 3.5-4.0 microg ml(-1)). Also, 54% of human leukemia (HL-60) cells were killed by the compound at 0.05 microg ml(-1). Bioreactor production demonstrated unusual primary metabolite kinetics. Molecular phylogenetic analysis showed this typical intertidal inhabitant to be a member of the Streptomyces genus and distinct from other salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic databases, our screening strategy should increase the possibility of discovering bioactive molecules from rare actinobacteria.

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Section: Resultsmentioning

confidence: 99%

Production and Purification of a Bioactive Substance Inhibiting Multiple Drug Resistant Bacteria and Human Leukemia Cells from a Salt-Tolerant Marine Actinobacterium sp. Isolated from the Bay of Bengal

et al. 2006

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“…One of the strategies to increase the possibility of discovering novel chemical entities is to screen actionmycetes considered rare in the extreme environments (Gathogo et al, 2004). Among these rare actinomycetes, the genus Actinomadura is reported to be the most predominant (Miyadoh, 1993).…”

Section: Introductionmentioning

confidence: 99%

Identification of a new strain of Actinomadura isolated from Saharan soil and partial characterization of its antifungal compounds

Badji¹,

Mostefaoui²,

Sabaou³

et al. 2011

Afr. J. Biotechnol.

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One promising strain Actinomadura sp. AC170, isolated from Algerian Saharan soil, with strong antifungal activity against pathogenic and toxinogenic fungi, was selected for further studies. The 16S rRNA results showed a distinct phylogenetic lineage from the other species within the Actinomadura genus. The production of antibiotic substances was investigated using GYEA solid medium. The butanolic extract contained four bioactive spots detected on thin layer chromatography plates. Among these antibiotics, a complex called 170A, which showed the more interesting antifungal activity, was selected and purified by reverse-phase HPLC. This complex is composed of four compounds. Ultraviolet-visible, infrared, mass and H nuclear magnetic resonance spectroscopy studies showed that these molecules contain an aromatic ring substituted by aliphatic chains. These compounds differ from the known antibiotics produced by Actinomadura species.

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Diversity and isolation of rare actinomycetes: an overview

Tiwari

Gupta

2012

Critical Reviews in Microbiology

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A renewed interest in the development of new antimicrobial agents is urgently needed to combat the increasing number of antibiotic-resistant strains of pathogenic microorganisms. Actinomycetes continue to be the mainstream supplier of antibiotics used in industry. The likelihood of discovering a new compound with novel chemical structure can be increased with intensive efforts in isolating and screening of rare genera of microorganisms to include in natural-product-screening collections. An unexpected variety of rare actinomycetes is now being isolated worldwide from previously uninvestigated diverse natural habitats, using different selective isolation methods. These isolation efforts include methods to enhance growth (enrichment) of rare actinomycetes, and eliminate unwanted microorganisms (pretreatment). To speed up the strain isolation process, knowledge about the distribution of such unexploited groups of microorganisms must also be augmented. This is a summary of using these microorganisms as new potential biological resources, and a review of almost all of the selective isolation methods, including pretreatment and enrichment techniques that have been developed to date for the isolation of rare actinomycetes.

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Rapid detection of `rare' actinomycetes in environmental samples

Cited by 3 publications

References 10 publications

Production and Purification of a Bioactive Substance Inhibiting Multiple Drug Resistant Bacteria and Human Leukemia Cells from a Salt-Tolerant Marine Actinobacterium sp. Isolated from the Bay of Bengal

Production and Purification of a Bioactive Substance Inhibiting Multiple Drug Resistant Bacteria and Human Leukemia Cells from a Salt-Tolerant Marine Actinobacterium sp. Isolated from the Bay of Bengal

Identification of a new strain of Actinomadura isolated from Saharan soil and partial characterization of its antifungal compounds

Diversity and isolation of rare actinomycetes: an overview

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