Rapid Detection of Pyuria by Leukocyte Esterase Activity

Kusumi, Rodney K.; Grover, Penelope J.; Kunin, Calvin M.

doi:10.1001/jama.1981.03310410031022

Cited by 92 publications

(32 citation statements)

References 16 publications

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“…The comparison of results between the dipstick and sediment analysis of WBCs done by the senior technolo- The results of the above study confirm the previously reported findings of the acceptable accuracy of the stick test in the detection of urinary leukocytes [1][2][3]. In addi tion, we also found that technologist bias is lesser when presence of WBCs is evaluated by the dipstick method as compared to sediment analysis.…”

supporting

confidence: 88%

“…Most laboratories rely on microscopic examination of the urine sediment. Recent studies have shown that a chemi cal dipstick test is accurate for detecting significant num bers of white cells in urine [1][2][3]. The purpose of the present study was to evaluate the degree of accuracy with which the stick test predicted the leukocyte concentration in urine as assessed by microscopic analysis of urine sediment.…”

mentioning

confidence: 95%

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Comparison between Sediment Analysis and a Simple Stick Test for Pyuria

Nanji¹,

Adam²,

Campbell³

1984

Nephron

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supporting

confidence: 88%

mentioning

confidence: 95%

Comparison between Sediment Analysis and a Simple Stick Test for Pyuria

Nanji¹,

Adam²,

Campbell³

1984

Nephron

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“…Studies have demonstrated VOL. 1,1988 a correlation between leukocyte esterase activity and the chamber count (43,71 …”

Section: Laboratory Analysesmentioning

confidence: 99%

Detection of urinary tract infections by rapid methods

Pezzlo

1988

Clin Microbiol Rev

121

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A review of rapid urine screens for detection of bacteriuria and pyuria demonstrates a number of available alternatives to the culture method. Selection of one or more of these systems for routine use is dependent upon the laboratory and the patient population being tested. The laboratory approach to the diagnosis of urinary tract infection should consider the clinical diagnosis of the patient whenever possible. Keeping in mind that quantitative urine cultures alone cannot be used to detect infection in some patient populations unless lower colony counts are considered, a rapid screen may be a more practical approach. It has become accepted that 10(5) CFU/ml can no longer be used as the standard for all patient groups, that pyuria often is important in making the diagnosis of a urinary tract infection, and that most of the rapid screens are more sensitive than the culture method at 10(5) CFU/ml. Presently, no one approach can be recommended for all laboratories and all patient groups. However, each diagnostic laboratory should select one approach which is best for its situation. It is not practical, efficient, or cost effective to define a protocol for each possible clinical condition; however, all should be considered when developing a protocol. This protocol should be compatible with the patient population and communicated to the physicians. Use of a rapid screen should be beneficial to the patient, the physician, and the laboratory.

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“…Values for sensitivity, specificity and predictive values show large variations for the different studies (table 6 a, b, c). Originally dipsticks with a leukocyte field requiring 15 minutes reaction time were used (2,5,6,7,8), whereas in later studies more sensitive methods (6), requiring 1-2 minutes reaction time were used (6, 12, 13, 14, 18, 21-25). …”

Section: Discussionmentioning

confidence: 99%

“…In others, special care was taken, such s the use of sterilized Containers and collection of clean voided, midstream or catheter urine (5,7,12,13,14,22).…”

Section: Discussionmentioning

confidence: 99%

Screening Urine Specimen Populations for Normality Using Different Dipsticks: Evaluation of Parameters Influencing Sensitivity and Specificity

Bank¹,

Codrington²,

Dieijen-Visser³

et al. 1987

Clinical Chemistry and Laboratory Medicine

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Summary:The reagent test strip Combur-9 Test-RL (Boehringer Mannheim) and the 8-SG Multistix (Ames) were simultaneously evaluated äs a rapid method for screening urines for normality.Differences between the two methods are for a considerable part determined by adjustment of the lowest detection limits of the leukocyte and erythrocyte dipstick fields.Patient populations (243 specimens presented to the routine laboratory and 230 specimens submitted for microbiological culture), Sediment analysis (routine or standardized) and composition of the screening protocol strongly influence values obtained for the sensitivity, specificity and predictive values, whereas use of a different dipstick is of minor importance on the final results.Higher sensitivity and specificity are observed when relating positive dipstick screening to positive culture than when relating positive standardized sediment to positive culture. Evaluation of dipstick method, using microscopic sediment analysis äs a reference parameter appears to be very dependent on the quality of the latter, which is therefore relatively unsuitable for this purpose. Apart from standardization, additional clinically significant findings are obtained using dipstick screening.

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Rapid Detection of Pyuria by Leukocyte Esterase Activity

Abstract: To evaluate whether leukocyte esterase activity is a useful biochemical marker of pyuria, we examined urine from 20 healthy volunteers, 47

Cited by 92 publications

References 16 publications

Comparison between Sediment Analysis and a Simple Stick Test for Pyuria

Comparison between Sediment Analysis and a Simple Stick Test for Pyuria

Detection of urinary tract infections by rapid methods

Screening Urine Specimen Populations for Normality Using Different Dipsticks: Evaluation of Parameters Influencing Sensitivity and Specificity

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