Rapid Detection of Piscirickettsia salmonis Using Microwave Irradiation.

Larenas, J.; Astorga, César; Contreras, Johanna; Garces, H; Fryer, J. L.; Smith, Pamela

doi:10.3147/jsfp.31.231

Cited by 8 publications

(4 citation statements)

References 2 publications

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“…Rainbow trout (ca 12 g) were obtained from a freshwater commercial farm located in a n area where piscirickettsiosis has never been reported (Metropolitan Region of Chile). To confirm the absence of Piscirickettsia salrnonis, kidney smears were tested by an indirect fluorescence antibody test (IFAT) according to the method of Lannan et al (1991), as modified by Larenas et al (1996a). A polyclonal P. salmonis antiserum, kindly provided by Mrs C. N. Lannan, from Oregon State University (USA) was used.…”

Section: Methodsmentioning

confidence: 99%

Routes of entry of Piscirickettsia salmonis in rainbow trout Oncorhynchus mykiss

Pa¹,

Pizarro²,

Ojeda³

et al. 1999

Dis. Aquat. Org.

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Since 1989, Piscirickettsia salmonis, the causal agent of piscirickettsiosis, has killed millions of farmed salrnonids each year in southern Chile. The portal of entry for the pathogen was investigated by use of selected experimental infections in juvenile rainbow trout (12 g). The methods used were intraperitoneal injection, subcutaneous injection, patch contact on skin, patch contact on gills, intestinal intubation and gastric intubation. Cumulative mortalities at Day 33 post-inoculation were 98, 100, 52, 24, 24, and 2 % , respectively. It was shown that intact skin and gills could be penetrated by P. salmonis. The high mortality obtained in subcutaneously injected fish indicated that s k i injunes could facilitate the invasion of this pathogen. Results suggested that the main entry sites are through the skin and gills and that the oral route may not be the normal method by which P. salmonis initiates infection of salmonids.

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Section: Methodsmentioning

confidence: 99%

Routes of entry of Piscirickettsia salmonis in rainbow trout Oncorhynchus mykiss

Pa¹,

Pizarro²,

Ojeda³

et al. 1999

Dis. Aquat. Org.

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“…The fluorescent antibody test can be used to confirm the presence of P. salmonis in smears and impressions directly from infected fish tissues, in suspect tissue cultures, and in paraffin‐embedded tissue sections (Lannan, Ewing & Fryer 1991; Larenas, Astorga, Contreras, Garcés, Fryer & Smith 1996a; Jamett et al. 2001).…”

Section: Confirmation Of P Salmonismentioning

confidence: 99%

“…Appropriately fixed preparations are incubated with rabbit anti‐ P. salmonis serum or mouse monoclonal antibodies and then with fluorescein or other dye‐conjugated antibodies directed to rabbit IgG. A similar test can be applied to deparaffinized tissue sections from infected fish with better results obtained if the sections are subjected to microwave irradiation to reveal P. salmonis antigens (Larenas et al. 1996a).…”

Section: Confirmation Of P Salmonismentioning

confidence: 99%

Piscirickettsia salmonis: a Gram‐negative intracellular bacterial pathogen of fish

Fryer

Hedrick

2003

Journal of Fish Diseases

173

142

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Piscirickettsia salmonis is the first Gram-negative, intracellular bacterial pathogen isolated from fish and is a significant cause of mortality in salmonid fish. Recent reports of P. salmonis or P. salmonis-like organisms from new fish hosts and geographic regions have increased the interest in the bacterium. In this review, the important characteristics of the bacterium including recent taxonomic changes, features of the disease caused by the bacterium including transmission, hosts, reservoirs, diagnostic procedures, and current approaches for prevention and treatment have been discussed. The reader is also directed to other reviews concerning the bacterium and the disease it causes (Fryer & Lannan 1994, 1996; Almendras & Fuentealba 1997; Lannan, Bartholomew & Fryer 1999; House & Fryer 2002; Mauel & Miller 2002).

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“…La prueba de inmunofluorescencia indirecta IFI desarrollada por Lannan y Fryer (1991) es la más utilizada por ser simple, de bajo costo, rápida y de adecuada sensibilidad para detectar la bacteria. En el microscopio de fluorescencia se le observa de color verde manzana y morfología cocoide, destacándose su contorno de forma de anillo (Larenas et al, 1996). La prueba IFI incorpora como primer anticuerpo inmunoglobulina IgG de ratón anti P. salmonis y como segundo anticuerpo un IgG comercial anti-ratón conjugado con isotiocianato de fluoresceína (FITC), aunque esta técnica fue modificada por Larenas et al (1996) mediante el uso de microondas, lo cual disminuye el tiempo para la incubación del primer y segundo anticuerpo.…”

Section: Introductionunclassified

Detección y asociación de Piscirickettsia salmonis en órganos y heces de salmón coho (Oncorhynchus kisutch) cultivado en el mar

Pongo

Papuico

Herrera

et al. 2021

Rev. investig. vet. Perú

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El objetivo de la investigación fue diagnosticar la presencia de Piscirickettsia salmonis en muestras de salmones coho (Oncorhynchus kisutch) provenientes de centros de cultivo de la Región de los Lagos, Chile, que presentaban brotes de piscirickettsiosis. Se emplearon 19 peces con signos clínicos de piscirickettsiosis. Se hicieron frotis de tejido renal, hepático, esplénico y de contenido intestinal que se procesaron mediante la prueba de inmunofluorescencia indirecta (IFI) para la detección de P. salmonis a través de un kit comercial. Los datos obtenidos se sometieron a la prueba de Q de Cochran y de McNemar en la distribución de Chi cuadrado. Se encontró mayor positividad en el hígado (68.4%) y riñón (52.6%). Además, se encontró la bacteria en las heces (47.4%), indicando que la eliminación vía fecal del agente es posible en condiciones de cultivo. Asociando los resultados de riñón e hígado solo de logra detectar el 78.9% de los peces positivos a la bacteria, en tanto que asociando los resultados de hígado, bazo y heces se logra detectar el 100% de los peces positivos a P. salmonis.

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Rapid Detection of Piscirickettsia salmonis Using Microwave Irradiation.

Cited by 8 publications

References 2 publications

Routes of entry of Piscirickettsia salmonis in rainbow trout Oncorhynchus mykiss

Routes of entry of Piscirickettsia salmonis in rainbow trout Oncorhynchus mykiss

Piscirickettsia salmonis: a Gram‐negative intracellular bacterial pathogen of fish

Detección y asociación de Piscirickettsia salmonis en órganos y heces de salmón coho (Oncorhynchus kisutch) cultivado en el mar

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