Rapid detection of mecA and nuc genes in staphylococci by real-time multiplex polymerase chain reaction

Costa, Anna‐Maria; Kay, Ian; Palladino, Silvano

doi:10.1016/j.diagmicrobio.2004.08.014

Cited by 94 publications

(57 citation statements)

References 25 publications

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“…Detection of MRSA colonization within 24 h would facilitate identification of carriers and allow the early implementation of isolation precautions for colonized patients in hopes of preventing spread to other patients. Several nucleic acid amplification-based assays for detection of MRSA from clinical specimens or for culture confirmation have been described (6,9,10,12,24,25,31). The IDI-MRSA assay (GenOhm, San Diego, CA) is a multiplex qualitative real-time PCR assay for detection of MRSA from nasal swabs.…”

mentioning

confidence: 99%

Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth

et al. 2006

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confidence: 99%

Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth

et al. 2006

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“…Methicillin-resistant Staphylococcus aureus (MRSA) was confirmed by penicillin-binding protein 2a detection or PCR for mecA and nuc if necessary (7).…”

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confidence: 99%

Cutaneous Diphtheria in the Urban Poor Population of Vancouver, British Columbia, Canada: a 10-Year Review

2011

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Between 1998 and 2007, records from 33 patients with cutaneous diphtheria from Vancouver's inner city were reviewed. Cases were associated with injection drug use and poverty. Coinfections with Staphylococcus aureus, Streptococcus pyogenes, and Arcanobacterium haemolyticum occurred. Corynebacterium diphtheriae is endemic in Vancouver's urban core, with strains of multilocus sequence type (MLST) 76 predominating.

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“…Extracted DNA subjected to 0.8% agarose gel electrophoresis then stained with Ethidium bromide. Purity and concentration determined by Nanodrop Spectrophotometer [7].…”

Section: Dna Extractionmentioning

confidence: 99%

“…The mecA gene used for detection of methicillin resistance [3]. Many researchers designed primers and probes for detection MRSA by real time PCR [5,6,7]. In silico approach was used in recent study to select the best primers and probe [8].…”

Section: Introductionmentioning

confidence: 99%

In Silico and in Vitro Evaluation of Real Time PCR Assay for Detection of Staphylococcus aureus

Al-Rawi¹,

Al-Kazaz²,

Alzubaidi³

2017

JNUS

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Many research groups developed real time PCR assays for identification of Methicillin-resistant Staphylococcus aureus (MRSA). They designed different primers and probes in their assays. Gene mecA is the target identification of MRSA by PCR assay. The aim of this study is using in silico approach to identify the best primers and probe for real time PCR identification mecA gene. Published primers and probes were analyzed in silico to select the best for real time PCR identification of mecA gene. The selected primers and probe successfully used for real time amplification of twenty MRSA tested. This study reveals the importance of in silico approach for designing diagnostic assays shorten the cost and the time.

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Rapid detection of mecA and nuc genes in staphylococci by real-time multiplex polymerase chain reaction

Cited by 94 publications

References 25 publications

Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth

Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth

Cutaneous Diphtheria in the Urban Poor Population of Vancouver, British Columbia, Canada: a 10-Year Review

In Silico and in Vitro Evaluation of Real Time PCR Assay for Detection of Staphylococcus aureus

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