Rapid detection of Listeria monocytogenes in foods, by a combination of PCR and DNA probe

Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

“…Only four proteins of the internalin family are part of the repertoire of secreted proteins, and among these, inlC is the most important. The gene encoding the inlC protein is present in pathogenic L. monocytogenes and L. ivanovii species, but not in other Listeria species (1) . The InlJ (or lmo2821) gene is responsible for passage of L. monocytogenes through the intestinal barrier and can be used for evaluating virulence of L. monocytogenes (5) .…”

“…L. monocytogenes infection in humans, listeriosis, is a serious illness that affects mostly immunosuppressed individuals, newborns, and the elderly, with symptoms ranging from septicemia, meningitis, encephalitis, abortions, to occasional death (1) . In recent years, many outbreaks of listeriosis have been implicated in the contamination of trading nutriments such as vegetables, milk, and meat foodstuffs (2) .…”

“…Polymerase chain reaction (PCR) is a rapid method with high sensitivity and specificity for specific deoxyribonucleic acid (DNA) sequences and permits direct detection of the pathogens (2) . A PCR employing amplification of the iap, prfA, hly, inl, and plcA gene sequences was recently reported for L. monocytogenes detection (1) . Liu et al showed that the inlA gene is species-specific and the inlC and inlJ genes are virulence-specific for L. monocytogenes (3) .…”

Emprego de um método molecular para avaliar a presença de Listeria monocytogenes em saladas de hortaliças folhosas minimamente processadas.

“…monocytogenes nos alimentos em geral (Zhang et al, 2004), o método de escolha deve ser muito sensível, capaz de detectar 1 célula / g do produto (Ingianni et al, 2001) e apresentar alta especificidade, prevenindo a ocorrência de resultados falsopositivos.…”

“…Um teste ótimo para utilização em rotina deve ser de fácil execução por técnicos não especializados, específico para L. monocytogenes, rápido, com a possibilidade de obter-se o resultado em poucas horas ou um dia, adequado para automação e não deve ter um custo elevado (Ingianni et al, 2001).…”