Rapid Detection of Human Viruses in Faeces by a Simple and Routine Immune Electron Microscopy Technique

Berthiaume, Laurent; Ahrends, Robert; McLaughlin, B.; Payment, Pierre; Trépanier, Pierre

doi:10.1099/0022-1317-55-1-223

Cited by 40 publications

(21 citation statements)

References 16 publications

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“…As mentioned before, the results of the present study should be viewed with caution specially for the presence of GARV and TTV, with respect to: i) the initial volume used for the viral concentration, since there are studies that use more than 500 mL and viruses may have been diluted when discharged into water bodies; ii) the methodology used for the concentration of GARV, as there are studies (Berthiaume et al, 1981;Smith and Gerba, 1982) which show that the use of positively charged membranes are more efficient for GARV recovery from water samples and iii) the limited analytical sensibility of conventional PCR when compared to Real-Time PCR. Taking this into consideration, the low number of samples positive for GARV may have due to the adsorption-elution protocol used for viral concentration, as well as the volume used in this process.…”

Section: Microbiological Parametersmentioning

confidence: 94%

“…The low rates for detection of GARV genomes may be associated to the methodology used for concentration of the samples, since other methods were used in the past for this purpose (Berthiaume et al, 1981;Smith and Gerba, 1982). Nevertheless, a number of recent reports described the use of adsorption-elution protocol for the detection of GARV in water and sewage samples (Li et al, 2011;Rigotto et al, 2010), although low percentages of rotavirus genomes were reported.…”

Section: Microbiological Parametersmentioning

confidence: 99%

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Assessment of enteric viruses in a sewage treatment plant located in Porto Alegre, southern Brazil

et al. 2012

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In order to verify the microbial quality of the influents and effluents of one STP from southern Brazil, an eight-month survey was conducted to examine the presence of total and fecal coliforms and of adenovirus (HAdV), enterovirus (EV), genogroup A rotaviruses (GARV) and Torque teno virus (TTV), in treated effluent samples from São João/ Navegantes STP, Porto Alegre (Brazil). A total of 16 samples were collected, eight of influent (raw sewage, prior to treatment), and the other eight of the effluent (post-treatment sewage). Total and fecal coliform levels ranging from 3.6 × 10 4 to 4.4 × 10 7 MPN/100 mL and 2.9 × 10 3 to 1.7 × 10 7 MPN/100 mL, were detected in all samples. In raw sewage, HAdV (25%) and GARV (28.6%) viral genomes were detected. The analysis of effluent samples revealed the presence of HAdV (50%), EV (37.5%), and TTV (12.5%) genomic fragments. All samples, regardless of the month analysed, presented detection of a least one virus genus, except for in April. Higher virus detection rates were observed in treated sewage samples (62.5%), and in 80% of them (effluent positive samples) HAdV was detected. Results showed that improvements in sewage monitoring and treatment processes are necessary to reduce the viral and bacterial load on the environment in southern Brazil. To the knowledge of the authors, this is the first study showing the monitoring of viral genomes in influent and effluent samples from a STP located in Porto Alegre (Rio Grande do Sul, Brazil), southern Brazil.

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Section: Microbiological Parametersmentioning

confidence: 94%

Section: Microbiological Parametersmentioning

confidence: 99%

Assessment of enteric viruses in a sewage treatment plant located in Porto Alegre, southern Brazil

et al. 2012

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“…In this technique, copper grids, previously prepared with collodion film and stabilized with carbon were first incubated with protein A (1ml/ml), placed in contact with a virus-specific antibody. After this, the grids were washed in PBS drops, incubated with the viral suspension washed with drops of water and negatively stained with 2% ammonium molybdate, pH 5.0 (Almeida & Waterson,1969;Derrick, 1973;Berthiaume et al, 1981).…”

Section: Negative Staining Technique (Rapid Preparation)mentioning

confidence: 99%

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

et al. 2013

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SUMMARY:Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillomatosis in four outbreaks in cattle herds, coming from Brazilian states were registered. The papillomatous lesions were found located in the teats, udders, head and neck. Under the transmission electron microscope, by the negative staining technique, it was possible to visualized rounded-format papillomavirus, with icosahedral symmetry, characterized as "full" and "empty" particles, measuring on average 60 nm in diameter, in all the 40 samples observed of skin lesion fragments. The ultrathin sections revealed the presence of groups of viral, intranuclear, rounded particles measuring 35 nm in diameter and tubular particles with a diameter of 35-39 nm. At immunoelectron microscopy technique, positivity obtained was marked by the presence of aggregates of viral particles formed by the antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction showed colloidal gold particles evenly distributed over the surface of the virus. These results showed the importance of the transmission electron microscopy techniques in the diagnosis of bovine papillomatosis that can be used in routine procedures to identify viral agent of this important disease.

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“…Several decades after the rotavirus description many studies have been carried out to compare detection techniques (11,(16)(17)(18)(19)(20)(21) . Currently, several commercial tests are available from less laborious based techniques that enable a quick diagnosis, e.g.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of antigen ELISA technique and PCR for detection of Rotavirus in stool samples of pediatric patients with diarrhea

Kumar¹

2018

jmscr

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Rapid Detection of Human Viruses in Faeces by a Simple and Routine Immune Electron Microscopy Technique

Cited by 40 publications

References 16 publications

Assessment of enteric viruses in a sewage treatment plant located in Porto Alegre, southern Brazil

Assessment of enteric viruses in a sewage treatment plant located in Porto Alegre, southern Brazil

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

Comparative evaluation of antigen ELISA technique and PCR for detection of Rotavirus in stool samples of pediatric patients with diarrhea

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