Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP)

Development of a reverse transcription loop-mediated isothermal amplification assay for visual detection of avian reovirus

“…The viral RNA can be detected by one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay performed with reverse transcriptase and LAMP reaction mixture. Currently, the assay is being widely used for the detection of various pathogens including viruses, bacteria and parasites (Thai et al, 2004;Pham et al, 2005;Poon et al, 2005;Curtis et al, 2008;Rovira et al, 2009;Xu et al, 2009;Yamazaki et al, 2009).…”

Development of a Reverse Transcriptase Loop-Mediated Isothermal Amplification (LAMP) Assay for the Sensitive Detection of Leishmania Parasites in Clinical Samples

“…19 Therefore, LAMP has emerged as a powerful tool for point-of-care diagnostics and has been implemented for other protozoan parasitic diseases such as the Plasmodium 20 , 21 and the Trypanosoma , 22 , 23 with high efficacy. Recently, several studies have included a reverse transcriptase step to specifically amplify RNA; this has mainly been used for amplification of RNA viruses such as human immunodeficiency virus (HIV) 24 and avian flu viruses, 25 moreover, by amplification of RNA it is possible to increase the sensitivity of the assay.…”

Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification

“…Although it has thus emerged as a powerful tool to facilitate genetic testing for the rapid diagnosis of several viral and bacterial infectious diseases in clinical laboratories, [2][3][4][5][6][7] the LAMP method has not been evaluated for the diagnosis of GMOs. In the present study, we developed a LAMP-based, highly sensitive, and specific technique to detect RR soybean and evaluated its sensitivity and specificity.…”