Rapid Detection of Hepatitis B Virus in Blood Plasma by a Specific and Sensitive Loop-Mediated Isothermal Amplification Assay

Nyan, Dougbeh-Chris; Ulitzky, Laura; Cehan, Nicoleta; Williamson, Phillip; Winkelman, Valerie; Rios, María; Taylor, Derek J.

doi:10.1093/cid/ciu210

Cited by 58 publications

(79 citation statements)

References 39 publications

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“…These results are in agreement with previously 331 published reports of (RT)-LAMP sensitivity and specificity. 21,24,28 332 This work utilized the 5 0 -NCR of the HCV genome for primer 333 design due to its conserved nature across HCV genotypes, thus 334 facilitating the detection of all HCV genotypes tested. This work also 335 exploited the sparse nucleotide polymorphism present within the 336 HCV 5 0 -NCR for genotype identification, as revealed by the 337 electrophoretic differences in banding patterns of the detected 338 targets ( Figure 1C).…”

Section: Primer Setmentioning

confidence: 99%

A method for rapid detection and genotype identification of hepatitis C virus 1–6 by one-step reverse transcription loop-mediated isothermal amplification

Nyan

Swinson

2016

International Journal of Infectious Diseases

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Section: Primer Setmentioning

confidence: 99%

A method for rapid detection and genotype identification of hepatitis C virus 1–6 by one-step reverse transcription loop-mediated isothermal amplification

Nyan

Swinson

2016

International Journal of Infectious Diseases

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“…; Nyan et al . ). To facilitate adoption of this tool, we provide a ‘know how’ guide to apply LAMP to projects where species identification or sex determination is needed in real time and in situ .…”

Section: Discussionmentioning

confidence: 97%

“…Because of its high specificity and sensitivity to the target region, isothermal conditions and easy detection, LAMP appeared as a promising tool in molecular techniques that has been already applied in medicine (Nyan et al . ), microbiology (Fukuta et al . ) and parasitology (Abbasi et al .…”

Section: Introductionmentioning

confidence: 99%

Sex determination in the wild: a field application of loop‐mediated isothermal amplification successfully determines sex across three raptor species

Centeno‐Cuadros

Abbasi

Nathan

2016

Molecular Ecology Resources

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18PCR-based methods are the most common technique for sex determination of birds. 19Although these methods are fast, easy and accurate, they still require special facilities 20 that preclude their application outdoors. Consequently, there is a time lag between 21 sampling and obtaining results that impedes researchers to take decisions in situ and in 22 real time considering individuals' sex. We present an outdoor technique for sex 23 determination of birds based on the amplification of the duplicated sex-chromosome-24 specific gene Chromo-Helicase-DNA binding protein using a Loop-Mediated 25Isothermal Amplification (LAMP). We tested our method on Griffon Vulture (Gyps 26 fulvus), Egyptian Vulture (Neophron percnopterus) and Black Kite (Milvus migrans) 27 (family Accipitridae). We introduce the first fieldwork procedure for sex determination 28 of animals in the wild, successfully applied to raptor species of three different 29 subfamilies using the same specific LAMP primers. This molecular technique can be 30 deployed directly in sampling areas since it only needs a voltage inverter to adapt a 31 thermo-block to a car lighter and results can be obtained by the unaided eye based on 32 colour change within the reaction tubes. Primers and reagents are prepared in advance 33 to facilitate their storage at room temperature. We provide detailed guidelines how to 34 implement this procedure, which is simpler (no electrophoresis required), cheaper and 35 faster (results in ca. 90 minutes) than PCR-based laboratory methods. Our successful 36 cross-species application across three different raptor subfamilies posits our set of 37 markers as a promising tool for molecular sexing of other raptor families and our field 38 protocol extensible to all bird species.

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“…Unfortunately no available POC NAAT platform yet includes, among the many testing options, kits for the combined screening of the three main blood-borne viruses. There are however promising and innovative NAAT technologies, such as the loop-mediated isothermal amplification assay (LAMP), with the potential to be sensitive, rapid and user-friendly enough to be utilized as a blood screening tool in remote settings [71].…”

Section: Transfusion Transmitted Infections -"Ttis"-screeningmentioning

confidence: 99%

Point of Care Testing and Transfusion Safety in Resource Limited Settings: A Review

Musi¹

2015

J Blood Disord Transfus

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Worldwide, one of the fastest growing aspects of clinical laboratory testing is point of care testing (POCT). Decentralized patient care and access to testing in under-served areas are key elements in the evolving expansion of POCT. Several available POCT devices can potentially contribute to safer transfusion practices in resourcelimited settings. Only "rapid diagnostic tests" and some simple hemoglobinometers are normally encountered in the peripheral hospitals of low income countries. Strengths, weaknesses and barriers of large scale utilization of POCT in transfusion medicine are discussed.

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Rapid Detection of Hepatitis B Virus in Blood Plasma by a Specific and Sensitive Loop-Mediated Isothermal Amplification Assay

Cited by 58 publications

References 39 publications

A method for rapid detection and genotype identification of hepatitis C virus 1–6 by one-step reverse transcription loop-mediated isothermal amplification

A method for rapid detection and genotype identification of hepatitis C virus 1–6 by one-step reverse transcription loop-mediated isothermal amplification

Sex determination in the wild: a field application of loop‐mediated isothermal amplification successfully determines sex across three raptor species

Point of Care Testing and Transfusion Safety in Resource Limited Settings: A Review

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