Rapid detection of Grapevine leafroll-associated virus type 3 using a reverse transcription loop-mediated amplification method

Walsh, Helen; Pietersen, Gerhard

doi:10.1016/j.jviromet.2013.08.030

Cited by 30 publications

(14 citation statements)

References 44 publications

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“…The RT-nPCR method is performed using the product of the first PCR as a template, and this can improve the sensitivity and specificity of detection. A recent study showed that RT-nPCR and RT-LAMP may have similar sensitivities for detection of GLRaV-3 type 3 [30]. Moreover, real-time PCR devices are not available in many laboratories in developing countries.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

et al. 2015

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Grapevine leafroll-associated virus 1 (GLRaV-1) is one of the causal agents of grapevine leafroll disease (GLD). To investigate the prevalence and genetic variation of GLRaV-1 in China, 132 grapevine samples from 14 Chinese provinces and regions were tested using reverse transcription PCR (RT-PCR) and reverse transcription nested PCR (RT-nPCR). The samples included symptomatic and asymptomatic cultivars, and 36.4% of them tested positive for GLRaV-1. 'Beida' samples, previously identified as virus-free rootstocks, were also found to be infected with GLRaV-1 with an incidence of 40 . GLRaV-1 coat protein (CP) genes and heat-shock protein 70 (HSP70) genes from 43 GLRaV-1 isolates were selected and sequenced. Phylogenetic analysis of global CP and HSP70 gene sequences showed that all variants belonged to eight and seven groups, respectively. For CP gene sequence variants, group 4 was a new group that included only Chinese isolates. The results also showed that natural selection, rather than random processes, led to the evolution of variants belonging to CP gene sequence variants in group 2 and group 8. Furthermore, three new recombination events were identified in the GLRaV-1 CP gene population. This is the first report on the genetic variation of GLRaV-1 isolates in China, and this study will benefit grape clean-plant programs in China.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

et al. 2015

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“…RT-PCR in combination with high-resolution melting curve analysis has been used for monitoring the incidence of GLRaVs and their variants in vineyards (19,135). Recently, a RT loop-mediated isothermal amplification assay was developed for the rapid detection of GLRaV-3 (163). Microarray-(50) and macroarray-(153) based detection methods have been developed for the multiplex detection of GLRaVs.…”

Section: Diagnosismentioning

confidence: 99%

Grapevine Leafroll: A Complex Viral Disease Affecting a High-Value Fruit Crop

et al. 2014

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Grapevine (Vitis spp.) is one of the most widely grown fruit crops in the world. It is a deciduous woody perennial vine for which the cultivation of domesticated species began approximately 6,000 to 8,000 years ago in the Near East. Grapevines are broadly classified into red- and white-berried cultivars based on their fruit skin color, although yellow, pink, crimson, dark blue, and black-berried cultivars also exist. Grapevines can be subject to attacks by many different pests and pathogens, including graft-transmissible agents such as viruses, viroids, and phytoplasmas. Among the virus and virus-like diseases, grapevine leafroll disease (GLD) is by far the most widespread and economically damaging viral disease of grapevines in many regions around the world. The global expansion of the grape and wine industry has seen a parallel increase in the incidence and economic impact of GLD. Despite the fact that GLD was recognized as a potential threat to grape production for several decades, our knowledge of the nature of the disease is still quite limited due to a variety of challenges related to the complexity of this virus disease, the association of several distinct GLD-associated viruses, and contrasting symptoms in red- and white-berried cultivars. In view of the growing significance of GLD to wine grape production worldwide, this feature article provides an overview of the state of knowledge on the biology and epidemiology of the disease and describes management strategies currently deployed in vineyards.

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“…To simplify PCR, a loop-mediated isothermal amplification (LAMP) method was proposed, which involves the amplification of specific DNA sequences using four or six primers [112]. The advantages of LAMP are fixed temperature conditions, high specificity, increased speed, visual detection, and resistance to inhibitors [113,114]. The sensitivity of this reaction is either higher than, or similar to that of PCR [107,114].…”

Section: Diagnostic Methods Based On Nucleic Acids Amplificationmentioning

confidence: 99%

Methods for the Diagnosis of Grapevine Viral Infections: A Review

et al. 2018

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The yielding capacity of grapevine growth and the quality of the resulting product heavily depend on the health of the cultivated plants. The phytopathogens affecting the vineyards can cause a significant reduction in the yield and quality of the product. For this reason, it is extremely important to use diagnostic methods that make it possible to identify the pathogens, and to choose the correct method of plant protection. This review considers the main viral grapevine pathogens, and the existing methods of their diagnosis. The limitations of conventional diagnostic methods that are based either on the visual assessment of symptoms, or on bio-testing, are analyzed. A major focus is placed on two intensively developed approaches of diagnosis, molecular genetic and immunochemical methods. Applications of amplification techniques and DNA chips are presented, as well as opportunities for next-generation sequencing. A reduction of assay duration and labor intensity in combination with the assay shifts from specialized laboratories toward the places of sampling are considered as the main factors influencing the development of immunodiagnostic techniques. The potential place of diagnostic tests in vine-growing practices, and the requirements for their most efficient applications for early disease diagnosis is also discussed.

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Rapid detection of Grapevine leafroll-associated virus type 3 using a reverse transcription loop-mediated amplification method

Cited by 30 publications

References 44 publications

Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

Grapevine Leafroll: A Complex Viral Disease Affecting a High-Value Fruit Crop

Methods for the Diagnosis of Grapevine Viral Infections: A Review

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