Rapid detection of fungi from blood samples of patients with candidemia using modified calcofluor white stain

Chen, Biao; Li, Wenqiang; Yin, Peng; Zhang, Ning; Bian, Shaodong; Liu, Chen; Yang, Zhiya; Jiang, Yufeng; Li, Renzhe; Ying-guang, Xie; Shi, Dongmei

doi:10.1016/j.mimet.2021.106202

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…To further characterise the reversible retraction of cytoplasm observed during hyphal space searching, we introduced the fluorescent dyes FM4-64 [75][76][77] and calcofluor white [78][79][80] into the AMF-SporeChip to stain hyphae. Both dyes stained the fungal hyphae successfully and confirmed the identity of emptied hyphae.…”

Section: Dynamic Hyphal Reactions Involving Reversible Cytoplasmic Re...mentioning

confidence: 99%

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Richter,

Calonne-Salmon,

van der Heijden

et al. 2024

Lab Chip

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Section: Dynamic Hyphal Reactions Involving Reversible Cytoplasmic Re...mentioning

confidence: 99%

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Richter,

Calonne-Salmon,

van der Heijden

et al. 2024

Lab Chip

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“…Los resultados obtenidos del presente estudio evidencian un porcen-⏐47 taje de positividad en la técnica de BCF de 72,2% que corresponde a 26 muestras, frente al 69,4% de positivos obtenidos en los cultivos micológicos, resultados similares fueron reportados por Biao et al (19). Incluso, con el avance de las tecnológicas, en los últimos años se ha intentado realizar microscopía de fluorescencia mediante teléfonos móviles para la identificación de patógenos fúngicos, lo cual podría ser útil con el propósito de realizar diagnósticos en lugares más apartados a bajo costo y de una manera simple (20).…”

Section: ⏐unclassified

Blanco de calcoflúor: en búsqueda de un mejor diagnóstico

Duque Restrepo,

Muñoz Monsalve,

Guerra Bustamante

et al. 2024

Rev. Médica Risaralda

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Introducción: Blanco de calcoflúor (BCF) es una tinción fluorescente que permite observar estructuras micóticas en distintas muestras clínicas gracias a la afinidad que tiene por la quitina. La microscopía en campo oscuro facilita la visualización correcta de los patógenos lo que favorece el diagnóstico oportuno y correcto de los pacientes. Por lo tanto, este trabajo tiene como objetivo evaluar la capacidad de identificación de estructuras micóticas en diferentes muestras biológicas de la coloración de blanco de calcoflúor. Materiales y métodos: se evaluaron 36 muestras biológicas (flujo vaginal, lavado broncoalveolar, líquido cefalorraquídeo, escamas, orina, córnea, hemocultivo y biopsia) en busca de hongos. Todas las muestras fueron procesadas por medio de las tres técnicas: hidróxido de potasio (KOH) al 20%, cultivo micológico y blanco de calcoflúor. Resultados: la técnica de KOH dio un resultado positivo en 58,3% de los casos, el cultivo en el 69,4% y la tinción con blanco de calcoflúor en el 72,2%. La sensibilidad y la especificidad de la técnica de BCF frente al KOH fue de 95% y 67% respectivamente, mientras que frente cultivo micológico fue de 100% y 91%. Conclusiones: este estudio demuestra que la técnica de BCF es un buen método para la identificación de estructuras micóticas en las muestras clínicas debido a que demostró unaalta sensibilidad y especificidad en relación con el método tradicional y el cultivo.

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“…Colonies grown on Middle-brook 7H10 agar media were stained by fluorescent calcofluor white (CFW) as previously described [26] and examined by fluorescence microscope. In addition, the isolates were also stained with acid-fast staining (the kit BA-4090B from Zhuhai Baso Biotechnology Company, China) as manufacturer instruction [27] and then observed under light microscope.…”

Section: The Isolation Of M Marinum Strainsmentioning

confidence: 99%

5-aminolevulinic acid-photodynamic therapy ameliorates cutaneous granuloma by killing drug-resistant Mycobacterium marinum

Yang

Feng

Pang

et al. 2021

Preprint

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Although 5-aminolevulinic acid photodynamic therapy (ALA-PDT) has been extensively used to treat to various skin disease, the application of ALA-PDT on cutaneous infection caused by Mycobacterium marinum (M. marinum), especially by drug-resistant M. marinum is still not clear. We evaluated the therapeutic efficacy of ALA-PDT on M. marinum in a mouse infection model and tested its killing effect on M. marinumin vitro. Finally, we investigated the clinical effect of ALA-PDT in treating cutaneous granuloma caused by drug-resistant M. marinum. We isolated total 9 strains of M. marinum from patients and confirmed by morphological and molecular approaches. The strains were identified by anti-mycobacterial susceptibility. Then we evaluated the killing effect of ALA-PDT on M. marinum in vitro and in a mouse model to observe the antimycobacterial effect of ALA-PDT. Therapeutic efficacy was further assessed in patients with cutaneous granuloma caused by drug-resistant M. marinum. We demonstrated that the ALA-PDT directly killed M. marinumin vitro. The paws cutaneous lesions of mice caused by M. marinum were fully recovered 2 weeks after ALA-PDT treatment. However, there was no significant difference for immune cells in peripheral blood before and after ALA-PDT therapy. Finally, ALA-PDT proved to be effective in treat two patients with cutaneous infection caused by drug-resistant M. marinum. The results suggest that ALA-PDT is effective in treating M. marinum cutaneous infections by killing M. marinum directly, independent of systemic immune responses. The data highlight the ALA-PDT as a promising therapeutic choice for M. marinum infection, especially for drug-resistant strains.

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Rapid detection of fungi from blood samples of patients with candidemia using modified calcofluor white stain

Cited by 6 publications

References 9 publications

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Blanco de calcoflúor: en búsqueda de un mejor diagnóstico

5-aminolevulinic acid-photodynamic therapy ameliorates cutaneous granuloma by killing drug-resistant Mycobacterium marinum

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