Rapid detection of fraudulence in seven commercial shrimp products by species-specific PCR assays

Rapid On-Site Identification for Three Arcidae Species (Anadara kagoshimensis, Tegillarca granosa, and Anadara broughtonii) Using Ultrafast PCR Combined with Direct DNA Extraction

“…japonicus ) with a detection limit of 0.005 ng [ 8 ]. Wilwet et al (2021) described a detection limit of 0.1 ng for seven commercial shrimp species ( Penaeus monodon, P. vannamei, P. semisulcatus, Fenneropenaeus indicus, Metapenaeus affinis, M. rosenbergii, and H. gibbosus ) [ 15 ]. Compared with previous studies, ultrafast PCR yielded a similar or two orders of magnitude higher sensitivity than that of conventional PCR methods.…”

“…Compared with previous studies, ultrafast PCR yielded a similar or two orders of magnitude higher sensitivity than that of conventional PCR methods. The lower detection limit of the PCR method makes it advantageous for processed seafood products [ 15 ]. Therefore, the suggested ultrafast PCR method could be used as a sensitive method to identify the three ark shell species as well as enable rapid detection using an ultrafast PCR system with the microfluidic chip.…”

“…The combination of these advantages allowed for the development of a method by which the entire procedure of sampling, DNA extraction, amplification, and data confirmation could be completed within approximately 25 min. The time required to detect seafood species in processed foods by using this method is less than that using other PCR-based methods [ 8 , 15 , 39 , 40 ]. Due to the advantages of quick analysis, ultrafast PCR or fast real-time PCR assays have been used to develop methods for the identification of economically motivated food adulteration [ 9 ].…”

“…PCR-based techniques, such as DNA barcoding, PCR restriction fragment length polymorphism, forensically informative nucleotide sequencing, and recombinase polymerase amplification, have been developed to detect different species [ 11 , 12 , 13 , 14 ]. These techniques have been used to identify commercially important seafood species, such as shrimps, cods, crabs, and oysters [ 8 , 15 , 16 , 17 ]. Recently, more rapid and convenient technologies have been developed to confirm the presence of target species in processed foods [ 18 , 19 ].…”

Rapid PCR-lateral flow assay for the onsite detection of Atlantic white shrimp

“…Species substitution is a violation of the Federal Food, Drug, and Cosmetic Act Section 403: Misbranded food, which states that “a food shall be deemed to be misbranded if it is offered for sale under the name of another food” ( FDA, 2020 ). Even though species substitution is against the law, shrimp species substitution rates as high as 34%, 16%, and 18% have been reported in Northern Carolina (USA), India, and South Africa, respectively ( Cawthorn and Hoffman, 2017 , Korzik et al, 2020 , Wilwet et al, 2021 ).…”